Protein Digestion
Choose Type:
- Which residues does Endoproteinase AspN cut?
- Are there any amino acid residues that inhibit or reduce the efficiency of digestion of glutamate residues in a peptide sequence with Endoproteinase GluC? The site I want to digest has a glutamate residue followed by a proline residue and some enzymes are inhibited by the presence of a proline after the hydrolysis site.
- I am using Trypsin and am wondering about specificity. Does it cut at additional sites when in high concentration?
- Is there a simple way to remove Trypsin after protein cleavage?
- How does one do a Trypsin in-gel digest?
- I have a very low concentration of protein and would prefer not to denature as a separate step with buffer exchange before digestion. What denaturants can he use in the Trypsin reaction itself?
- What is the Proteinase K activity in commonly used buffers?
- O-Glycosidase Application Note 1 (P0733)
- O-Glycosidase (P0733)
- Endo-α-N-Acetylgalactosaminidase Application Note 1
- Removal of terminal N-acetylglucosamine from the biantennary N-linked sugars of IgG
- Typical Reaction Conditions (P0732)
- Typical Reaction Conditions for β1-4 Galactosidase (P0730)
- RNase B Deglycosylation Protocol (P7817)
- Protocol using Trypsin-ultra™, Mass Spectrometry Grade (P8101)
- Trypsin Digestion Protocol using NEB Trypsin-ultra™ and the FASP Kit
- Typical Reaction Conditions for β1-4 Galactosidase S (P0745)
- Typical Reaction Conditions for α2-3 Neuraminidase S (P0743)
- Typical Reaction Conditions for β-N-Acetylglucosaminidase S (P0744)
- Reaction Protocols for Protein Deglycosylation Mix II (P6044)
- In-gel Digestion Protocol for Endoproteinase LysC (P8109)
- Typical Reaction Conditions for Endoproteinase LysC
- Typical GluC Digest Reaction Conditions (P8100)
- Typical GluC In-Gel Digest Reaction Conditions (P8100)
- Typical Trypsin and GluC Co-Digest Reaction Conditions (P8100)
- Glycoproteomics Brochure
- Glycoproteomics Technical Guide
Brochures
Products and content are covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB). The use of trademark symbols does not necessarily indicate that the name is trademarked in the country where it is being read; it indicates where the content was originally developed. The use of this product may require the buyer to obtain additional third-party intellectual property rights for certain applications. For more information, please email busdev@neb.com.
This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.