Proteomics
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- Which residues does Endoproteinase AspN cut?
- Are there any amino acid residues that inhibit or reduce the efficiency of digestion of glutamate residues in a peptide sequence with Endoproteinase GluC? The site I want to digest has a glutamate residue followed by a proline residue and some enzymes are inhibited by the presence of a proline after the hydrolysis site.
- I am using Trypsin and am wondering about specificity. Does it cut at additional sites when in high concentration?
- Is there a simple way to remove Trypsin after protein cleavage?
- How does one do a Trypsin in-gel digest?
- I have a very low concentration of protein and would prefer not to denature as a separate step with buffer exchange before digestion. What denaturants can he use in the Trypsin reaction itself?
- What is the Proteinase K activity in commonly used buffers?
- Cleavage of the Fusion Protein Generated Using The pMAL Protein Fusion and Purification System (E8200)
- Cloning a PCR Fragment Into a pMAL Expression Vector (E8200)
- O-Glycosidase Application Note 1 (P0733)
- O-Glycosidase (P0733)
- Purification of a Fusion Protein generated by The pMAL Protein Fusion and Purification System (E8200)
- Reaction Protocols for Protein Deglycosylation Mix (P6039)
- Separating the Protein of Interest from MBP after Protease Cleavage Using The pMAL Protein Fusion and Purification System (E8200)
- Western Transfer Protocol for Anti-MBP Monoclonal Antibody
- Endo-α-N-Acetylgalactosaminidase Application Note 1
- Quick Start Guide (E8200)
- Removal of terminal N-acetylglucosamine from the biantennary N-linked sugars of IgG
- Typical Reaction Conditions for α2-3,6,8 Neuraminidase (P0720)
- Endo H/Endo Hf Protocol
- Typical Reaction Conditions (P0732)
- Typical Reaction Conditions for α2-3 Neuraminidase (P0728)
- Typical Reaction Conditions for β1-4 Galactosidase (P0730)
- Reaction Conditions for Remove-iT® PNGase F (P0706)
- Reaction Conditions for Endo S (P0741)
- Endo S Removal Magnetic Chitin Bead Protocol (P0741)
- Remove-iT® PNGase F Magnetic Chitin Bead Protocol (P0706)
- Reaction Conditions for Endo D (P0742)
- Endo D Removal Magnetic Chitin Bead Protocol (P0742)
- RNase B Deglycosylation Protocol (P7817)
- PNGase F Protocol
- Typical Reaction Conditions for α2-3,6,8,9 Neuraminidase A (P0722)
- Typical Reaction Conditions for α1-3, 4, 6 Galactosidase (P0747)
- Typical Reaction Conditions for α1-2, 3, 4, 6 Fucosidase (P0748)
- Typical Reaction Conditions for α1-3, 4 Fucosidase (P0769)
- Reaction Conditions for IdeZ Protease (IgG-specific) (P0770)
- Reaction conditions for Simultaneous Digestion of IgG with IdeZ Protease (IgG-specific) and PNGase F (fragmentation and deglycosylation) (P0770)
- Glycoproteomics: Buffer Exchange Protocols (P0711)
- Rapid PNGase F (non-reducing format) (P0711) Reaction Protocol
- Rapid PNGase F (non-reducing format) (P0711) SDS-PAGE Protocol
- Typical Reaction Conditions for Endo F3 Protocol (P0771)
- Reaction Conditions for PNGase A (P0707)
- In-gel Digestion Protocol for Endoproteinase LysC (P8109)
- Typical Reaction Conditions for Endoproteinase LysC
- Endo F2 Reaction Protocol (P0772)
- Removal of Endo F2 by Magnetic Beads (P0772)
- a1-2,4,6 Fucosidase O Digestion of Released Labeled Glycans Protocol
- Glycoproteomics Brochure
- Glycoproteomics Technical Guide
- Rapid PNGase F Brochure
Brochures
- Leith, E.M., O'Dell, W.B., Ke, N., McClung, C., Berkmen, M., Bergonzo, C., Brinson, R.G., Kelman, Z (2019) Characterization of the internal translation initiation region in monoclonal antibodies expressed in Escherichia coli J Biol Chem; 294(48), 18046-18056.. PubMedID: 31604819, DOI: 10.1074/jbc.RA119.011008
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