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Glycobiology

New England Biolabs offers a variety of endo- and exoglycosidases for the modification and removal of glycans from glycoproteins in glycobiology research. We also offer heparin lyases, also known as heparinases, for the digestion of heparin and heparan sulfate.

New England Biolabs (NEB) offers a selection of endoglycosidases, exoglycosidases, and heparinases for glycobiology research. Many of these reagents are recombinant, and all undergo several quality control assays, enabling us to provide products with lower unit cost, high purity and reduced lot-to-lot variation. All of our glycosidases are tested for contaminants. Since p-nitrophenyl-glycosides are not hydrolyzed by some exoglycosidases, we use only fluorescently-labeled oligosaccharides to screen for contaminating glycosidases.

To find all of our Glycobiology Application Notes, click here.

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Glycobiology

Endoglycosidases

Exoglycosidases

Heparinases

Glycoprotein Substrates

Glycobiology Buffers & Diluents

Endoglycosidases

Exoglycosidases

Heparinases

Glycoprotein Substrates

Glycobiology Buffers & Diluents

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Glycobiology includes these subcategories:

Endoglycosidases
Exoglycosidases
Heparinases
Glycoprotein Substrates
Glycobiology Buffers & Diluents

FAQs for Glycobiology

Application Notes for Glycobiology

Comprehensive structural and positional profiling of intact complex O-glycans in biologic drugs using O-Glycoprotease (IMPa)

Tools & Resources

Feature Articles

The Structure, Function and Importance of Carbohydrates

Read about the structure, function, and importance of Carbohydrates from biology experts at NEB.

Using Glycosidases to Remove Trim or Modify Glycans on Therapeutic Protein

O-glycan analysis of therapeutic proteins enabled by O-glycoprotease

This article from European Pharmaceutical Review describes tools that can be used for O-glycan analysis of therapeutic proteins.

Brochures

Glycoproteomics Brochure

Glycoproteomics Technical Guide

Purification Beads, Columns and Resins Brochure

Rapid PNGase F Brochure

Web Tools

Glycan Analyzer

Selection Tools

Endoglycosidase Selection Chart

Usage Guidelines

Detailed Characterization of Several Glycosidase Enzymes

Glycobiology Unit Conversion Chart

Posters

Improving Sample Workflow: Rapid PNGase F for Accurate Antibody Characterization (2015)

Publications related to Glycobiology

Kalidas, C., Joshi, L., Batt, C. (2001) Characterization of glycosylated variants of beta-lactoglobulin expressed in Pichia pastoris Protein Eng; 14 , 201-7 . PubMedID: 11342718
Whistler, J.L., Enquist, J., Marley, A., Fong, J., Gladher, F., Tsuruda, P., Murray, S.R., Von Zastrow, M. (2002) Modulation of postendocytic sorting of G protein-coupled receptors Science; 297 , 615-20 . PubMedID: 12142540
Oh, D.B., Park, J.S., Kim, M.W., Cheon, S.A., Kim, E.J., Moon, H.Y., Kwon, O., Rhee, S.K., Kang, H.A. (2008) Glycoengineering of the methylotrophic yeast Hansenula polymorpha for the production of glycoproteins with trimannosyl core N-glycan by blocking core oligosaccharide assembly Biotechnol J; 3 , 659-68 . PubMedID: 18320567
Macnaughtan, M.A., Tian, F., Liu, S., Meng, L., Park, S., Azadi, P., Moremen, K.W., Prestegard, J.H. (2008) 13C-sialic acid labeling of glycans on glycoproteins using ST6Gal-I J Am Chem Soc; 130 , 11864-5 . PubMedID: 18700760
Park, Y., Zhang, Z., Laremore, T.N., Li, B., Sim, J.S., Im, A.R., Ahn, M.Y., Kim, Y.S., Linhardt, R.J., Glycoconj, J. (2008) Variation of acharan sulfate and monosaccharide composition and analysis of neutral N-glycans in African giant snail (Achatina fulica) Glycoconj J; 25 , 863-77 . PubMedID: 18670878
Fujita, N., Tamura, A., Higashidani, A., Tonozuka, T., Freeze, H.H., Nishikawa, A. (2008) The relative contribution of mannose salvage pathways to glycosylation in PMI-deficient mouse embryonic fibroblast cells FEBS J; 275 , 788-98 . PubMedID: 18215164
Zhuo, Y., Chammas, R., Bellis, S.L. (2008) Sialylation of beta1 integrins blocks cell adhesion to galectin-3 and protects cells against galectin-3-induced apoptosis J Biol Chem; 283 , 22177-85 . PubMedID: 18676377
Wagner-Rousset, E., Bednarczyk, A., Bussat, M.C., Colas, O., Corvaïa, N., Schaeffer, C., Van Dorsselaer, A., Beck, A. (2008) The way forward, enhanced characterization of therapeutic antibody glycosylation: comparison of three level mass spectrometry-based strategies J Chromatogr B Analyt Technol Biomed Life Sci; 872, 23-37. PubMedID: 18672411
Graham, D.R., Mitsak, M.J., Elliott, S.T., Chen, D., Whelan, S.A., Hart, G.W., Van Eyk, J.E. (2008) Two-dimensional gel-based approaches for the assessment of N-Linked and O-GlcNAc glycosylation in human and simian immunodeficiency viruses Proteomics; 872, 23-37. PubMedID: 19072736
Banala J. et al. (2008) Caged substrates for protein labeling and immobilization Chembiochem; 4 , 38-41 . PubMedID: 18033718
Baudino L, Shinohara Y, Nimmerjahn F, Furukawa J, Nakata M, Martínez-Soria E, Petry F, Ravetch JV, Nishimura S, Izui S. (2008) Crucial role of aspartic acid at position 265 in the CH2 domain for murine IgG2a and IgG2b Fc-associated effector functions J Immunol; 181(9) , 6664-9 . PubMedID: 18941257
Teuton, J.R., Brandt, C.R. (2007) Sialic acid on herpes simplex virus type 1 envelope glycoproteins is required for efficient infection of cells J Virol; 81 , 3731-9 . PubMedID: 17229687
Albrecht, S., Vainauskas, S., Stöckmann, H., McManus, C., Taron, C.H. and Rudd, P.M. (2016) Comprehensive Profiling of Glycosphingolipid Glycans Using a Novel Broad Specificity Endoglycoceramidase in a High-Throughput Workflow. Anal Chem; May 3;88(9), 4795-802. Anal Chem.. PubMedID: 27033327
Song, Y., Choi, M.H., Park, J.N., Kim, M.W., Kim, E.J., Kang, H.A., Kim, J.Y. (2007) Engineering of the yeast Yarrowia lipolytica for the production of glycoproteins lacking the outer-chain mannose residues of N-glycans Appl Environ Microbiol; 73 , 4446-54 . PubMedID: 17513593
Aoki, K., Perlman, M., Lim, J.M., Cantu, R., Wells, L., Tiemeyer, M. (2007) Dynamic developmental elaboration of N-linked glycan complexity in the Drosophila melanogaster embryo J Biol Chem; 282 , 9127-42 . PubMedID: 17264077
Velho, A.M., Jarvis, S.M. (2009) Topological studies of hSVCT1, the human sodium-dependent vitamin C transporter and the influence of N-glycosylation on its intracellular targeting Exp Cell Res; 315 , 2312-21 . PubMedID: 19379732
Gefter, J.V., Shaufl, A.L., Fink, M.P., Delude, R.L. (2009) Comparison of distinct protein isoforms of the receptor for advanced glycation end-products expressed in murine tissues and cell lines Cell Tissue Res; 337 , 79-89 . PubMedID: 19415334
Lee, K.J., Jung, J.H., Lee, J.M,, So, Y., Kwon, O., Callewaert, N., Kang, H.A., Ko, K., Oh, D.B. (2009) High-throughput quantitative analysis of plant N-glycan using a DNA sequencer Biochem Biophys Res Commun; 380 , 223-9 . PubMedID: 19167352
Boeggeman, E., Ramakrishnan, B., Pasek, M., Manzoni, M., Puri, A., Loomis, K.H., Waybright, T.J., Qasba, P.K. (2009) Site specific conjugation of fluoroprobes to the remodeled Fc N-glycans of monoclonal antibodies using mutant glycosyltransferases: application for cell surface antigen detection Bioconjugate Chem; 20 , 1228-36 . PubMedID: 19425533
Gong, B., Cukan, M., Fisher, R., Li, H., Stadheim, T.A., Gerngross, T. (2009) Characterization of N-linked glycosylation on recombinant glycoproteins produced in Pichia pastoris using ESI-MS and MALDI-TOF Methods Mol Biol; 534 , 213-23 .
Piro, J.R., Harris, B.T., Nishina, K., Soto, C., Morales, R., Rees, J.R., Supattapone, S. (2009) Prion protein glycosylation is not required for strain-specific neurotropism J Virol; 83 , 5321-8 .
Rasmussen, T.N., Plenge, P., Bay, T., Egebjerg, J., Gether, U. (2009) A single nucleotide polymorphism in the human serotonin transporter introduces a new site for N-linked glycosylation Neuropharmacology; 57 , 287-94 . PubMedID: 19500602
Lee, K.J., Jung, J.H., Lee, J.M., So, Y., Kwon, O., Callewaert, N., Kang, H.A., Ko, K., Oh, D.B. (2009) High-throughput quantitative analysis of plant N-glycan using a DNA sequencer Biochem Biophys Res Commun; 380 , 223-9 . PubMedID: 19167352
Eichler, R., Lenz, O., Garten, W., Strecker, T. (2006) The role of single N-glycans in proteolytic processing and cell surface transport of the Lassa virus glycoprotein.GP-C. Virol J; 3 , 41 . PubMedID: 16737539
Sielaff I. et al. (2006) Protein function microarrays based on self-immobilizing and self-labeling fusion proteins Chembiochem; 7 , 194-202 . PubMedID: 16342318
O’Reilly, M.K., Zhang, G., Imperiali, B. (2006) In vitro evidence for the dual function of Alg2 and Alg11: essential mannosyltransferases in N-linked glycoprotein biosynthesis Biochemistry; 8 , 9593-603 . PubMedID: 16878994
Hagen, B.M., Sanders, K.M. (2006) Deglycosylation of the beta1-subunit of the BK channel changes its biophysical properties Am J Physiol Cell Physiol; 1 , 750-6 . PubMedID: 16738006
Lüftenegger, D., Picard-Maureau, M., Stanke, N., Rethwilm, A., Lindemann, D. (2005) Analysis and function of prototype foamy virus envelope N glycosylation J Virol; 79 , 7664-72 . PubMedID: 15919919
Srikrishna, G., Brive, L., Freeze, H.H. (2005) Novel carboxylated N-glycans contain oligosaccharide-linked glutamic acid Biochem Biophys Res Commun; 332 , 1020-7 . PubMedID: 15922305
Cipollo, J.F., Awad, A.M., Costello, C.E., Hirschberg, C.B. (2005) N-Glycans of Caenorhabditis elegans are specific to developmental stages J Biol Chem; 280 , 26063-72 . PubMedID: 15899899
Möykkynen T, Coleman SK, Semenov A, Keinänen K (2014) The N-terminal domain modulates α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA) receptor desensitization J Biol Chem; 289(19), 13197-205. PubMedID: 24652293, DOI: 10.1074/jbc.M113.526301
Kwon HM, Lee KH, Han BW, Han MR, Kim DH, Kim DE (2014) An RNA aptamer that specifically binds to the glycosylated hemagglutinin of avian influenza virus and suppresses viral infection in cells PLoS One; 9(5), e97574. PubMedID: 24835440, DOI: 10.1371/journal.pone.0097574
Wright CR, Brown EL, Della-Gatta PA, Ward AC, Lynch GS, Russell AP (2014) G-CSF does not influence C2C12 myogenesis despite receptor expression in healthy and dystrophic skeletal muscle Front Physiol; 5, 170. PubMedID: 24822049, DOI: 10.3389/fphys.2014.00170
Itahana Y, Han R, Barbier S, Lei Z, Rozen S, Itahana K (2014) The uric acid transporter SLC2A9 is a direct target gene of the tumor suppressor p53 contributing to antioxidant defense Oncogene; PubMedID: 24858040, DOI: 10.1038/onc.2014.119
Botto L, Cunati D, Coco S, Sesana S, Bulbarelli A, Biasini E, Colombo L, Negro A, Chiesa R, Masserini M, Palestini P (2014) Role of lipid rafts and GM1 in the segregation and processing of prion protein PLoS One; 9(5), e98344. PubMedID: 24859148, DOI: 10.1371/journal.pone.0098344
Rosenbaek LL, Kortenoeven ML, Aroankins TS, Fenton RA (2014) Phosphorylation decreases ubiquitylation of the thiazide-sensitive cotransporter NCC and subsequent clathrin-mediated endocytosis J Biol Chem; 289(19), 13347-61. PubMedID: 24668812, DOI: 10.1074/jbc.M113.543710
Haller G, Li P, Esch C, Hsu S, Goate AM, Steinbach JH (2014) Functional characterization improves associations between rare non-synonymous variants in CHRNB4 and smoking behavior PLoS One; 9(5), e96753. PubMedID: 24804708, DOI: 10.1371/journal.pone.0096753
Arakel EC, Brandenburg S, Uchida K, Zhang H, Lin YW, Kohl T, Schrul B, Sulkin MS, Efimov IR, Nichols CG, Lehnart SE, Schwappach B (2014) Tuning the electrical properties of the heart by differential trafficking of KATP ion channel complexes J Cell Sci; 127(Pt 9), 2106-19. PubMedID: 24569881, DOI: 10.1242/jcs.141440
Rosenbaum EE, Vasiljevic E, Brehm KS, Colley NJ (2014) Mutations in four glycosyl hydrolases reveal a highly coordinated pathway for rhodopsin biosynthesis and N-glycan trimming in Drosophila melanogaster PLoS Genet; 10(5), e1004349. PubMedID: 24785692, DOI: 10.1371/journal.pgen.1004349
Vainauskas, S., Kirk, C.H., Petralia, L., Guthrie, E.P., McLeod, E., Bielik, A., Luebbers, A., Foster, J.M., Hokke, C.H., Rudd, P.M., Shi, X., Taron, C.H (2018) A novel broad specificity fucosidase capable of core a1-6 fucose release from N-glycans labeled with urea-linked fluorescent dyes Sci Rep; PubMedID: 29934601, DOI: 1038/s41598-018-27797-0
O'Flaherty, Roisin, Harbison, Aoife M., Hanley, Philip J., Fadda, Elisa, Rudd, Pauline, Taron, Chris (2017) Aminoquinoline fluorescent labels obstruct efficient removal of N-glycan corex J Proteome Res; 16, 4237-4243. PubMedID: 28953389
Stech M, Quast RB, Sachse R, Schulze C, Wüstenhagen DA, Kubick S (2014) A continuous-exchange cell-free protein synthesis system based on extracts from cultured insect cells PLoS One; 9(5), e96635. PubMedID: 24804975, DOI: 10.1371/journal.pone.0096635
Wicht O, Burkard C, de Haan CA, van Kuppeveld FJ, Rottier PJ, Bosch BJ (2014) Identification and Characterization of a Proteolytically Primed Form of the Murine Coronavirus Spike Proteins after Fusion with the Target Cell J Virol; 88(9), 4943-52. PubMedID: 24554652, DOI: 10.1128/JVI.03451-13
Zhao H, Blazanovic K, Choi Y, Bailey-Kellogg C, Griswold KE (2014) Gene and protein sequence optimization for high-level production of fully active and aglycosylated lysostaphin in Pichia pastoris Appl Environ Microbiol; 80(9), 2746-53. PubMedID: 24561590, DOI: 10.1128/AEM.03914-13

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Videos

Overview of Glycobiology

Learn about the core sequences and common modifications of N-linked and O-linked glycans in this video. Analysis of these glycans can be accomplished with the use of deglycosylation enzymes, which can provide complete sugar removal with no protein degradation.
Identification and Characterization of Protein Glycosylation

Learn how glycosidases are used to analyze multiple glycoprotein samples. Here, the model glycoprotein, hCGβ, which carries both N- and O-glycans, is demonstrated.
Behind the Paper: An engineered Fbs1 carbohydrate binding protein for selective capture of N-glycans and N-glycopeptides

Minyong and Jim summarize their recent Nature Communications publication describing selective capture of N-glycans and N-glycopeptides by an engineered high affinity Fbs1 carbohydrate binding protein.
Elucidating the Complexity of Heparin Oligosaccharide Analysis

Learn more about the structural elucidation of complex and diverse heparin oligosaccharides using Bacteroides Heparinase I, II and III in combination with downstream mass spec analysis.
One-step protocol for deglycosylation with Rapid PNGase F

This one-step deglycosylation protocol is for use with New England Biolabs’ Rapid PNGase F. Learn more about Rapid PNGase F.
Two-step protocol for deglycosylation with Rapid PNGase F

This two-step deglycosylation protocol is for use with New England Biolabs’ Rapid PNGase F. Learn more about Rapid PNGase F.