PmeI

The following reagents are supplied with this product:

NEB #	Component Name	Component #	Stored at (°C)	Amount	Concentration
R0560S     -20       PmeI R0560SVIAL -20 1 x 0.05 ml 10,000 units/ml   rCutSmart™ Buffer B6004SVIAL -20 1 x 1.25 ml 10 X   Gel Loading Dye, Purple (6X) B7024AVIAL 25 1 x 0.5 ml 6 X
R0560L     -20       PmeI R0560LVIAL -20 1 x 0.25 ml 10,000 units/ml   rCutSmart™ Buffer B6004SVIAL -20 1 x 1.25 ml 10 X   Gel Loading Dye, Purple (6X) B7024AVIAL 25 1 x 0.5 ml 6 X

Materials Sold Separately

• rCutSmart™ Buffer

1. This enzyme is shown to have lower activity on some supercoiled plasmids, with more than 1 unit required to digest 1ug plasmid DNA. For complete digestion of 1ug plasmid DNA please follow our recommended digestion protocol.
   
2. Based on the stability of the enzyme in the reaction, incubations longer than 1 hr will not result in improved digestion, unless additional enzyme is added. Please refer to Restriction endonuclease survival in a reaction for more information regarding this topic.
3. Blocked by some combinations of overlapping CpG methylation.

1. Optimizing Restriction Endonuclease Reactions
2. Restriction Digest Protocol
3. Double Digest Protocol with Standard Restriction Enzymes

• Activity at 37°C for Restriction Enzymes with Alternate Incubation Temperatures
• Activity of Restriction Enzymes in PCR Buffers
• Cleavage Close to the End of DNA Fragments
• Digestion of Agarose-Embedded DNA: Info for Specific Enzymes
• Double Digests
• Effects of CpG Methylation on Restriction Enzyme Cleavage
• Heat Inactivation
• NEBuffer Activity/Performance Chart with Restriction Enzymes
• Optimizing Restriction Endonuclease Reactions
• Restriction Endonucleases - Survival in a Reaction
• Restriction Enzyme Diluent Buffer Compatibility
• Restriction Enzyme Tips
• Single Letter Codes
• Star Activity
• Traditional Cloning Quick Guide

• Alphabetized List of Recognition Sequences
• Compatible Cohesive Ends and Generation of New Restriction Sites
• Dam-Dcm and CpG Methylation
• Isoschizomers
• NEB Diluent and Buffer Table
• Recleavable Blunt Ends
• Time-Saver™ Qualified Enzymes
• Why Choose Recombinant Enzymes?
• 制限部位の頻度

• Competitor Cross-Reference Tool
• DNA配列およびマップ

• Double Digest Finder
• Enzyme Finder
• NEBcutter™ v3.0
• NEBioCalculator®
• REBASE®

1. How can I improve PmeI digestion of genomic DNA embedded in agarose?
2. What is Star Activity and how can it be avoided?
3. Do I have to set-up digests with Time-Saver™ qualified enzymes for 5-15 minutes? Can I digest longer?
4. I tested your restriction enzyme on the substrate DNA recommended by NEB, and it appears to be active, however it does not digest my DNA.  What could be the reason?
5. Which NEB restriction enzymes are supplied with Gel Loading Dye, Purple (6X)?
6. Is this enzyme sensitive to dam, dcm or mammalian CpG methylation?
7. Can you tell me more about the switch from BSA to Recombinant Albumin (rAlbumin) in NEBuffers?

• Restriction Enzyme Troubleshooting Guide

品質管理試験はNEB製品の新しいロットごとに実施され、指定された仕様に適合することを確認している。品質管理試験内容は製品仕様書、分析証明書、データカードまたは製品マニュアルに記載されている。NEB製品の品質に関する詳細はこちら をご覧ください。

The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]

• R0560S_L_v1
• R0560S_L_E_v2

The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]

• R0560S_L_v1_0161212
• R0560S_L_v1_0161305
• R0560S_L_v1_0161311
• R0560S_L_v1_0161405
• R0560S_L_v1_0161410
• R0560S_L_v1_0161504
• R0560S_L_v1_0161509
• R0560S_L_v1_0161605
• R0560S_L_v1_0171701
• R0560S_L_v1_0171706
• R0560S_L_v1_0171711
• R0560S_v1_10014142
• R0560L_v1_10014140
• R0560S_v1_10021377
• R0560L_v1_10028126
• R0560S_v1_10028121
• R0560L_v1_10037290
• R0560S_v1_10036629
• R0560L_v1_10043209
• R0560S_v1_10043208
• R0560S_v1_10050185
• R0560S_v1_10052628
• R0560S_v1_10056013
• R0560S_v1_10061234
• R0560S_v1_10064225
• R0560L_v1_10063048
• R0560L_v1_10068966
• R0560S_v1_10068584
• R0560S_v1_10077060
• R0560S_v1_10081003
• R0560L_v1_10080215
• R0560S_v1_10082943
• R0560L_v1_10091137
• R0560S_v1_10087057
• R0560S_v1_10092285
• R0560L_v1_10086400
• R0560S_v1_10103315
• R0560L_v1_10108640
• R0560S_v1_10107831
• R0560S_v1_10112123
• R0560S_v1_10119704
• R0560S_v1_10124305
• R0560L_v1_10134730
• R0560S_v1_10137533
• R0560L_v1_10139635
• R0560S_v1_10140692
• R0560S_v1_10145348
• R0560L_v1_10151154
• R0560S_v1_10152603
• R0560L_v1_10157702
• R0560S_v1_10157704
• R0560S_v1_10165588
• R0560L_v1_10165595
• R0560S_v1_10171845
• R0560L_v1_10179251
• R0560L_v1_10182963
• R0560S_v1_10179250
• R0560L_v1_10187155
• R0560S_v1_10187154
• R0560S_v1_10196738
• R0560L_v1_10196736
• R0560S_v1_10209067
• R0560S_v1_10196741
• R0560L_v1_10213824
• R0560L_v2_10223608
• R0560S_v2_10223606
• R0560S_v2_10235039
• R0560S_v2_10241911
• R0560L_v2_10242052
• R0560S_v2_10259710
• R0560S_v2_10266148
• R0560L_v2_10276960
• R0560S_v2_10287442
• R0560S_v2_10286971
• R0560S_v2_10305642
• R0560L_v2_10310878
• R0560S_v2_10312925
• R0560S_v2_10324914
• R0560S_v2_10339202
• R0560L_v2_10341596

The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.

• PmeI
• rCutSmart™ Buffer
• Gel Loading Dye, Purple (6X)

製品および内容は、New England Biolabs, Inc (NEB)が所有または管理する1つまたは複数の特許、 商標 および/または著作権によって保護されている。商標記号の使用は、必ずしもその名称が読まれている国で商標登録されていることを示すものではなく、内容が最初に開発された場所を示すものである。本製品を使用することにより、購入者は特定の用途について第三者の知的財産権を追加取得する必要が生じる場合がある。詳細については、 tech.jp@neb.com .

まで電子メールでお問い合わせください。 本製品は研究目的でのみ使用できる。本製品は、ヒトまたは動物における治療または診断目的での使用を意図したものではない。

New England Biolabs (NEB)は、倫理的な科学の実践に取り組んでいる。私たちは、その答えが得られれば、私たちの生活の質および私たちの住む世界の保全に役立つ重要な疑問を投げかけることが、研究者としての私たちの仕事であると考えている。ただし、この研究は常に安全で倫理的な方法で行われるべきである。 詳細を見る.