NEBNext® Multiplex Oligos for Illumina® (Unique Dual Index UMI Adaptors DNA Set 1)

For details on how to combine UMI adaptors with our NEBNext library prep kits, click the product information tab below.

NEBNext® Multiplex Oligos for Illumina® are an essential piece of the NEBNext suite of library preparation products, available in several different configurations for use with NEBNext products or other standard Illumina-compatible library preparation protocols. This set was designed and optimized for use in DNA sequencing workflows, including PCR-free. These unique dual index adaptors also include a 12-nt unique molecular identifier (UMI) sequence to enable the identification and removal of PCR errors or duplicates from amplified libraries. Supplied as 96 unique, pre-annealed index adaptors with UMIs, packaged in a single-use 96-well plate with a pierceable foil seal.  Universal primers are included for applications requiring PCR amplification.

Three other sets of Unique Dual Index UMI Adaptors are available for DNA library prep (NEB #E7874, NEB #E7876, and NEB #E7878), providing sufficient indices to multiplex up to 384 samples. NEBNext Unique Dual Index UMI Adaptors for RNA-seq (NEB # E7416) are also available, as well as single, dual, and unique dual index primers, plus an optimized adaptor.

For additional information on available indices and formats for multiplexing, please refer to the NEBNext Multiplex Oligos Selection Chart

  • Index adaptors enable PCR-free library preparation
  • Superior and reliable ligation efficiency
  • More-sensitive detection of low-frequency single-nucleotide variants (SNVs)
  • Supports multiplexing for higher throughputs
Catalog # Concentration Size List Price Your Price Quantity
E7395S Not Applicable 96 reactions $723.00
E7395L Not Applicable 384 reactions $2,749.00
Please enter a quantity for at least one size

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