NEBNext® Multiplex Oligos for Illumina® (Unique Dual Index UMI Adaptors DNA Set 1)
For details on how to combine UMI adaptors with our NEBNext library prep kits, click the product information tab below.
NEBNext® Multiplex Oligos for Illumina® are an essential piece of the NEBNext suite of library preparation products, available in several different configurations for use with NEBNext products or other standard Illumina-compatible library preparation protocols. This set was designed and optimized for use in DNA sequencing workflows, including PCR-free. These unique dual index adaptors also include a 12-nt unique molecular identifier (UMI) sequence to enable the identification and removal of PCR errors or duplicates from amplified libraries. Supplied as 96 unique, pre-annealed index adaptors with UMIs, packaged in a single-use 96-well plate with a pierceable foil seal. Universal primers are included for applications requiring PCR amplification.
Three other sets of Unique Dual Index UMI Adaptors are available for DNA library prep (NEB #E7874, NEB #E7876, and NEB #E7878), providing sufficient indices to multiplex up to 384 samples. NEBNext Unique Dual Index UMI Adaptors for RNA-seq (NEB # E7416) are also available, as well as single, dual, and unique dual index primers, plus an optimized adaptor.
NEBNext® Multiplex Oligos for Illumina® (Unique Dual Index UMI Adaptors DNA Set 1) are built for more streamlined and informative NGS multiplexing, tracking the sequencing reaction at the individual molecule level and enabling bioinformatic removal of misassigned reads for optimal accuracy. These index adaptors contain a pair of unique dual indices (i5 and i7) and one UMI sequence, making the resulting library multiplex-ready, with or without PCR. This set includes 96 unique dual index UMI adaptors, packaged in a single-use 96-well plate with a pierceable foil seal. The universal primer mix is supplied in a separate vial.
Combining UMI Adaptors DNA Set 1 with one of the NEBNext DNA library prep kits is simple and straightforward if you follow the protocols found in the library prep kit manuals. Quickly find the correct manual by selecting which library prep kit/module you plan to use:
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