FAQ: What are the main applications for the NEBNext Unique Dual Index UMI Adaptors DNA Sets 1-4?

The NEBNext Unique Dual Index UMI Adaptors DNA Sets 1-4 are recommended for applications that require PCR-free library preparation and sample pooling prior to PCR amplification. In addition, the incorporation of a 12 base unique molecular identifier (UMI) allows 1) accurate identification and removal of duplicate reads, and 2) consensus sequence building and error correction, ideally suited for accurate analysis of quantitative NGS data including low level frequency mutation detection.

Furthermore, the Unique Dual Index UMI Adaptors were designed to facilitate sequencing on patterned flow cell for the Illumina instruments where index hopping is problematic. The unique combination of dual indices allows identification and complete filtering of index-swapped reads.