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    NEBNext® Multiplex Oligos for Illumina® (Index Primers Set 1)

    Description

    The NEBNext Multiplex Oligos for Illumina (Index Primers 1–12) contains adaptors and primers that are ideally suited for multiplex sample preparation for next-generation sequencing on the Illumina platform (Illumina, Inc.). Each of these components must pass rigorous quality control standards and is lot controlled, both individually and as a set of reagents.

    Figure 1: Workflow.
    Functional Validation
    Each set of reagents is functionally validated together through construction and sequencing of a DNA library on the Illumina sequencing platform.

    Lot Control

    The lots provided are managed separately and qualified by additional functional validation. Individual reagents undergo standard enzyme activity and quality control assays, and also meet stringent criteria in the additional quality controls listed on each individual component page

    Reagents Supplied

    The following reagents are supplied with this product:

    Store at (°C)Concentration
    NEBNext Adaptor for Illumina-20
    USER Enzyme
    NEBNext Universal PCR Primer for Illumina-20
    NEBNext Index 1 Primer for Illumina
    NEBNext Index 2 Primer for Illumina
    NEBNext Index 3 Primer for Illumina
    NEBNext Index 4 Primer for Illumina
    NEBNext Index 5 Primer for Illumina
    NEBNext Index 6 Primer for Illumina
    NEBNext Index 7 Primer for Illumina
    NEBNext Index 8 Primer for Illumina
    NEBNext Index 9 Primer for Illumina
    NEBNext Index 10 Primer for Illumina
    NEBNext Index 11 Primer for Illumina
    NEBNext Index 12 Primer for Illumina

    Properties and Usage

    Materials Required but not Supplied

    • Enzymes and buffers appropriate for DNA, ChIP or mRNA Library Preparation (NEB #E6000, E6040, E6056, E6100, E6110, E6200, E6240, E7420, E7445, E7530, E7370)
    • AMPure® XP Beads (Beckman Coulter, Inc.) or PCR Column Purification Kit (Qiagen or other) 
    • Size Selection Materials [E-Gel® (Life Technologies, Inc.), Agarose Gel or AMPure XP Beads] 
    • DNA LoBind Tubes (Eppendorf®)/PCR Plates 
    • Magnetic Stand 
    • Bioanalyzer® (Agilent Technologies, Inc.) 
    • Freshly Prepared 80% Ethanol
    Please Refer to the Kit Specific Protocol for using the NEBNext Singleplex Oligos for Illumina:

    The following kits are designed for use with the NEBNext Singleplex Oligos for Illumina:
    • #E7420, NEBNext Ultra Directional RNA Library Prep Kit for Illumina
    • #E7530, NEBNext Ultra RNA Library Prep Kit for Illumina
    • #E7370, NEBNext Ultra DNA Library Prep Kit for Illumina
    • #E7445, NEBNext Ultra Ligation Module
    • #E6040, NEBNext DNA Library Prep Master Mix Set for Illumina
    • #E6110, NEBNext mRNA Library Prep Master Mix Set for Illumina
    • #E6240, NEBNext ChIP-Seq Library Prep Master Mix Set for Illumina
    • #E6056, NEBNext Quick Ligation Module
    • #E6000, NEBNext DNA Library Prep Reagent Set for Illumina
    • #E6200, NEBNext ChIP-Seq Library Prep Reagent Set for Illumina
    • #E6100, NEBNext mRNA Library Prep Reagent Set for Illumina

    Storage Temperature

    -20°C

    Supporting Documents

    Material Safety Datasheets

    The following is a list of Material Safety Data Sheets (MSDS) that apply to this product to help you use it safely. The following file naming structure is used to name these document files: [Product Name] MSDS. For international versions please contact us at info@neb.com.

    Manuals

    The Product Manual includes details for how to use the product, as well as details of its formulation and quality controls. The following file naming structure is used to name these document files: manual[Catalog Number].
    1. Are NEBNext adaptor and primers for Illumina validated in Next Generation sequencing workflows?
    2. Are NEBNext adaptor and primers for Illumina compatible with NEBNext reagents for Illumina library preparation?
    3. Is USER enzyme required for library preparation using NEBNext adaptor and primers for Illumina?
    4. How much DNA can I ligate to the NEBNext adaptor for Illumina in one ligation reaction?
    5. Are these reagents available in a customized format? Larger format or 96 well format?