NEBNext® Multiplex Oligos for Illumina® (Index Primers Set 1)

NEBNext^® Multiplex Oligos for Illumina^® (Index Primers Set 1)

NEBNext Multiplex Oligos provide adaptors and primers to enable high yield multiplex Illumina library production. The unique hairpin loop structure of the NEBNext Adaptor minimizes adaptor-dimer formation, and NEBNext index PCR primers enable index incorporation during library amplification.

For additional information on available indices and formats for multiplexing, please refer to the NEBNext Multiplex Oligos Selection Chart.

High efficiency adaptor ligation
Minimized adaptor-dimer formation
Includes index primers for library multiplexing

Note: NEBNext^® Multiplex Oligos for Illumina^® (Index Primer Set 1) (NEB #7335S/L) includes 12 indices.

Catalog #	Concentration	Size	List Price	Your Price
E7335S	Not Applicable	24 reactions (12 indices)	$107.00	Sign In Sign In
E7335L	Not Applicable	96 reactions (12 indices)	$391.00	Sign In Sign In

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Product Information

Incorporating a novel hairpin loop structure, the NEBNext Adaptor ligates with increased efficiency to end-repaired, dA-tailed DNA. The loop contains a U, which is removed by treatment with USER™ Enzyme (a mix of UDG and Endo VIII), to open up the loop and make it available as a substrate for PCR. During PCR, barcodes can be incorporated by use of the NEBNext index primers, thereby enabling multiplexing.

NEBNext Oligos can be used with NEBNext products, and with other standard Illumina-compatible library preparation protocols.

Additional single- or dual-barcode primer options are available, in 12- and 96- index formats (NEB #E7500, NEB #E7710, NEB #E7730, NEB #E6609, NEB #E7600). A methylated version of the NEBNext Adaptor is also available for use with bisulfite sequencing protocols (NEB #E7535).

Kit Components

The following reagents are supplied with this product:

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NEB # Component Name Component # Stored at (°C) Amount Concentration

E7335S

-20

NEBNext Adaptor for Illumina	E7337AVIAL	-20	1 x 0.24 ml	Not Applicable
USER^® Enzyme	E7338AVIAL	-20	1 x 0.072 ml	Not Applicable
NEBNext Universal PCR Primer for Illumina	E6861AVIAL	-20	1 x 0.12 ml	10 µM
NEBNext Index 1 Primer for Illumina	E7311AVIAL	-20	1 x 0.01 ml	10 µM
NEBNext Index 2 Primer for Illumina	E7312AVIAL	-20	1 x 0.01 ml	10 µM
NEBNext Index 3 Primer for Illumina	E7313AVIAL	-20	1 x 0.01 ml	10 µM
NEBNext Index 4 Primer for Illumina	E7314AVIAL	-20	1 x 0.01 ml	10 µM
NEBNext Index 7 Primer for Illumina	E7317AVIAL	-20	1 x 0.01 ml	10 µM
NEBNext Index 8 Primer for Illumina	E7318AVIAL	-20	1 x 0.01 ml	10 µM
NEBNext Index 9 Primer for Illumina	E7319AVIAL	-20	1 x 0.01 ml	10 µM
NEBNext Index 10 Primer for Illumina	E7320AVIAL	-20	1 x 0.01 ml	10 µM
NEBNext Index 11 Primer for Illumina	E7321AVIAL	-20	1 x 0.01 ml	10 µM
NEBNext Index 12 Primer for Illumina	E7322AVIAL	-20	1 x 0.01 ml	10 µM
NEBNext Index 6 Primer for Illumina	E7316AVIAL	-20	1 x 0.01 ml	10 µM
NEBNext Index 5 Primer for Illumina	E7315AVIAL	-20	1 x 0.01 ml	10 µM

E7335L

-20

NEBNext Adaptor for Illumina	E7337AAVIAL	-20	1 x 0.96 ml	Not Applicable
USER^® Enzyme	E7338AAVIAL	-20	1 x 0.288 ml	Not Applicable
NEBNext Universal PCR Primer for Illumina	E6861AAVIAL	-20	1 x 0.48 ml	10 µM
NEBNext Index 1 Primer for Illumina	E7311AAVIAL	-20	1 x 0.04 ml	10 µM
NEBNext Index 2 Primer for Illumina	E7312AAVIAL	-20	1 x 0.04 ml	10 µM
NEBNext Index 3 Primer for Illumina	E7313AAVIAL	-20	1 x 0.04 ml	10 µM
NEBNext Index 4 Primer for Illumina	E7314AAVIAL	-20	1 x 0.04 ml	10 µM
NEBNext Index 5 Primer for Illumina	E7315AAVIAL	-20	1 x 0.04 ml	10 µM
NEBNext Index 6 Primer for Illumina	E7316AAVIAL	-20	1 x 0.04 ml	10 µM
NEBNext Index 7 Primer for Illumina	E7317AAVIAL	-20	1 x 0.04 ml	10 µM
NEBNext Index 8 Primer for Illumina	E7318AAVIAL	-20	1 x 0.04 ml	10 µM
NEBNext Index 9 Primer for Illumina	E7319AAVIAL	-20	1 x 0.04 ml	10 µM
NEBNext Index 10 Primer for Illumina	E7320AAVIAL	-20	1 x 0.04 ml	10 µM
NEBNext Index 11 Primer for Illumina	E7321AAVIAL	-20	1 x 0.04 ml	10 µM
NEBNext Index 12 Primer for Illumina	E7322AAVIAL	-20	1 x 0.04 ml	10 µM