NEBNext® Multiplex Oligos for Illumina® (Index Primers Set 3)
NEBNext® Multiplex Oligos provide adaptors and primers to enable high yield multiplex Illumina library production. The unique hairpin loop structure of the NEBNext Adaptor minimizes adaptor-dimer formation, and NEBNext index PCR primers enable index incorporation during library amplification.
Additional NEBNext Multiplex Oligos products are available with sets of 12 and 96 single-index primers as well as dual-index primers.
- High efficiency adaptor ligation
- Minimized adaptor-dimer formation
- Includes index primers for library multiplexing
12 Quick Tips for NGS Library Preparation
NEBNext for Core Lab Facilities
NEB TV Ep. 4 – Next Generation Sequencing
Incorporating a novel hairpin loop structure, the NEBNext Adaptor ligates with increased efficiency to end-repaired, dA-tailed DNA. The loop contains a U, which is removed by treatment with USER® Enzyme (a mix of UDG and Endo VIII), to open up the loop and make it available as a substrate for PCR. During PCR, barcodes can be incorporated by use of the NEBNext index primers, thereby enabling multiplexing.
NEBNext Oligos can be used with NEBNext products, and with other standard Illumina-compatible library preparation protocols.
Additional single- or dual-barcode primer options are available, in 12- and 96- index formats (NEB #E7335, NEB #E7500, NEB #E7730, NEB #E6609, NEB #E7600). A methylated version of the NEBNext Adaptor is also available for use with bisulfite sequencing protocols (NEB #E7535).
For larger volume requirements, customized and bulk packaging is available by purchasing through the Custom Solutions department at NEB. Please contact OEM@neb.com for further information.
Figure 1: Workflow demonstrating the use of NEBNext Multiplex Oligos for Illumina (Index Primers Set 3)
The following reagents are supplied with this product:
Store at (°C) Concentration NEBNext Universal PCR Primer for Illumina NEBNext Adaptor for Illumina -20 USER® Enzyme -20 NEBNext Index 17 Primer for Illumina -20 NEBNext Index 24 Primer for Illumina -20 NEBNext Index 26 Primer for Illumina -20 NEBNext Index 28 Primer for Illumina -20 NEBNext Index 29 Primer for Illumina -20 NEBNext Index 31 Primer for Illumina -20 NEBNext Index 32 Primer for Illumina -20 NEBNext Index 33 Primer for Illumina -20 NEBNext Index 34 Primer for Illumina -20 NEBNext Index 35 Primer for Illumina -20 NEBNext Index 36 Primer for Illumina -20 NEBNext Index 42 Primer for Illumina -20
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