NEBNext® Multiplex Oligos provide adaptors and primers to enable high yield multiplex Illumina® library production. The unique hairpin loop structure of the NEBNext Adaptor minimizes adaptor-dimer formation, and NEBNext index PCR primers enable index incorporation during library amplification.
Additional NEBNext Multiplex Oligos products are available with sets of 12- and 96- single index primers as well as dual index primers.
Incorporating a novel hairpin loop structure, the NEBNext Adaptor ligates with increased efficiency to end-repaired, dA-tailed DNA. The loop contains a U, which is removed by treatment with USER™ Enzyme (a mix of UDG and Endo VIII), to open up the loop and make it available as a substrate for PCR. During PCR, barcodes can be incorporated by use of the NEBNext index primers, thereby enabling multiplexing.
NEBNext Oligos can be used with NEBNext products, and with other standard Illumina-compatible library preparation protocols.
Additional single- or dual-barcode primer options are available, in 12- and 96- index formats (NEB #E7335, NEB #E7710, NEB #E7730, NEB #E6609, NEB #E7600). A methylated version of the NEBNext Adaptor is also available for use with bisulfite sequencing protocols (NEB #E7535).
For larger volume requirements, customized and bulk packaging is available by purchasing through the OEM/Bulks department at NEB. Please contact custom[email protected] for further information.
Each set of reagents is functionally validated together through construction and sequencing of genomic DNA libraries on the Illumina sequencing platform.
The following reagents are supplied with this product:
|Store at (°C)||Concentration|
|NEBNext Universal PCR Primer for Illumina||-20|
|NEBNext Index 13 Primer for Illumina||-20|
|NEBNext Index 14 Primer for Illumina||-20|
|NEBNext Index 15 Primer for Illumina||-20|
|NEBNext Index 18 Primer for Illumina||-20|
|NEBNext Index 19 Primer for Illumina||-20|
|NEBNext Index 20 Primer for Illumina||-20|
|NEBNext Index 21 Primer for Illumina||-20|
|NEBNext Index 22 Primer for Illumina||-20|
|NEBNext Index 23 Primer for Illumina||-20|
|NEBNext Index 25 Primer for Illumina||-20|
|NEBNext Index 27 Primer for Illumina||-20|
|NEBNext Adaptor for Illumina||-20|
|NEBNext® Index 16 Primer for Illumina®|
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