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  • NEBNext® Multiplex Oligos for Illumina® (Index Primers Set 2)

    Description






    The NEBNext Multiplex Oligos for Illumina (Index Primers Set 2) contains adaptors and primers that are ideally suited for multiplex sample preparation for next-generation sequencing on the Illumina platform (Illumina, Inc.). Each of these components must pass rigorous quality control standards and is lot controlled, both individually and as a set of reagents.

    Figure 1: Workflow.

    Functional Validation
    Each set of reagents is functionally validated together through construction and sequencing of a DNA library on the Illumina sequencing platform.

    Lot Control

    The lots provided are managed separately and qualified by additional functional validation. Individual reagents undergo standard enzyme activity and quality control assays, and also meet stringent criteria in the additional quality controls listed on each individual component page

    Reagents Supplied

    The following reagents are supplied with this product:

    Store at (°C)Concentration
    NEBNext Universal PCR Primer for Illumina-20
    NEBNext Index 13 Primer for Illumina-20
    NEBNext Index 14 Primer for Illumina-20
    NEBNext Index 15 Primer for Illumina-20
    NEBNext Index 16 Primer for Illumina-20
    NEBNext Index 18 Primer for Illumina-20
    NEBNext Index 19 Primer for Illumina-20
    NEBNext Index 20 Primer for Illumina-20
    NEBNext Index 21 Primer for Illumina-20
    NEBNext Index 22 Primer for Illumina-20
    NEBNext Index 23 Primer for Illumina-20
    NEBNext Index 25 Primer for Illumina-20
    NEBNext Index 27 Primer for Illumina-20
    NEBNext Adaptor for Illumina-20
    USER Enzyme-20

    Properties and Usage

    Materials Required but not Supplied

    • Enzymes and buffers appropriate for DNA, CHiP or mRNA Library Preparation (NEB #E6000, E6040, E6056, E6100, E6110, E6200, E6240, E7420, E7445, E7530, E7370)
    • AMPure® XP Beads (Beckman Coulter, Inc.) or PCR Column Purification Kit (Qiagen or other)
    • Size Selection Materials [E-Gel® (Life Technologies, Inc.), Agarose Gel or AMPure XP Beads]
    • DNA LoBind® Tubes (Eppendorf®)/PCR Plates
    • Magnetic Stand
    • Bioanalyzer® (Agilent Technologies, Inc.)
    • Freshly Prepared 80% Ethanol

    Storage Temperature

    -20°C

    FAQs

    1. Are NEBNext adaptor and primers for Illumina validated in Next Generation sequencing workflows?
    2. Are NEBNext adaptor and primers for Illumina compatible with NEBNext reagents for Illumina library preparation?
    3. Is USER enzyme required for library preparation using NEBNext adaptor and primers for Illumina?
    4. How much DNA can I ligate to the NEBNext adaptor for Illumina in one ligation reaction?
    5. Are these reagents available in a customized format, larger format or 96 well format?
    6. Can USER Enzyme be purchased separately?
    7. Are the NEBNext Oligos for Illumina compatible with single end and paired end sequencing?
    8. Is the USER enzyme in our NEBNext kits identical in concentration to the standalone product?

    Protocols

    1. Please see manual (NEB #E7500) for protocols

    Manuals

    The Product Manual includes details for how to use the product, as well as details of its formulation and quality controls. The following file naming structure is used to name these document files: manual[Catalog Number].

    Safety Data Sheet

    The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.