NEBNext® Multiplex Oligos for Illumina® (Dual Index Primers Set 2)
NEBNext Multiplex Oligos provide adaptors and primers to enable high yield multiplex Illumina library production. The unique hairpin loop structure of the NEBNext Adaptor minimizes adaptor-dimer formation, and NEBNext index PCR primers enable index incorporation during library amplification. This kit includes 8 i5 index primers and 12 i7 index primers for dual indexing.
Additional NEBNext Multiplex Oligos products are available with sets of 12- and 96- single index primers as well as dual index primers.
- High efficiency adaptor ligation
- Minimized adaptor-dimer formation
- Includes index primers for library multiplexing
- Increased sample identification specificity
Incorporating a novel hairpin loop structure, the NEBNext Adaptor ligates with increased efficiency to end-repaired, dA-tailed DNA. The loop contains a U, which is removed by treatment with USER® Enzyme (a mix of UDG and Endo VIII), to open up the loop and make it available as a substrate for PCR. During PCR, barcodes can be incorporated by use of the NEBNext index primers, thereby enabling multiplexing. This kit includes 8 i5 index primers and 12 i7 index primers.
NEBNext Oligos can be used with NEBNext products, and with other standard Illumina-compatible library preparation protocols.
Additional dual index primer options are available (NEB #E7600). Single primer options are also available, in 12- and 96- index formats (NEB #E7335, NEB #E7500, NEB #E7710, NEB #E7730, NEB #E6609). A methylated version of the NEBNext Adaptor is also available for use with bisulfite sequencing protocols (NEB #E7535).
For larger volume requirements, customized and bulk packaging is available by purchasing through the OEM/Bulks department at NEB. Please contact [email protected] for further information.
The following reagents are supplied with this product:
Store at (°C) Concentration NEBNext Adaptor for Illumina -20 USER® Enzyme -20 NEBNext® i509 Primer -20 NEBNext® i510 Primer -20 NEBNext® i511 Primer -20 NEBNext® i512 Primer -20 NEBNext® i513 Primer -20 NEBNext® i514 Primer -20 NEBNext® i515 Primer -20 NEBNext® i516 Primer -20 NEBNext® i713 Primer -20 NEBNext® i714 Primer -20 NEBNext® i715 Primer -20 NEBNext® i716 Primer -20 NEBNext® i717 Primer -20 NEBNext® i718 Primer -20 NEBNext® i719 Primer -20 NEBNext® i720 Primer -20 NEBNext® i721 Primer -20 NEBNext® i722 Primer -20 NEBNext® i723 Primer -20 NEBNext® i724 Primer -20
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