NEBNext® Multiplex Oligos for Illumina® (Dual Index Primers Set 2)
NEBNext Multiplex Oligos provide adaptors and primers to enable high yield multiplex Illumina library production. The unique hairpin loop structure of the NEBNext Adaptor minimizes adaptor-dimer formation, and NEBNext index PCR primers enable index incorporation during library amplification. This kit includes 8 i5 index primers and 12 i7 index primers for dual indexing.
Additional NEBNext Multiplex Oligos products are available with sets of 12- and 96- single index primers as well as dual index primers.
- High efficiency adaptor ligation
- Minimized adaptor-dimer formation
- Includes index primers for library multiplexing
- Increased sample identification specificity
Incorporating a novel hairpin loop structure, the NEBNext Adaptor ligates with increased efficiency to end-repaired, dA-tailed DNA. The loop contains a U, which is removed by treatment with USER® Enzyme (a mix of UDG and Endo VIII), to open up the loop and make it available as a substrate for PCR. During PCR, barcodes can be incorporated by use of the NEBNext index primers, thereby enabling multiplexing. This kit includes 8 i5 index primers and 12 i7 index primers.
NEBNext Oligos can be used with NEBNext products, and with other standard Illumina-compatible library preparation protocols.
Additional dual index primer options are available (NEB #E7600). Single primer options are also available, in 12- and 96- index formats (NEB #E7335, NEB #E7500, NEB #E7710, NEB #E7730, NEB #E6609). A methylated version of the NEBNext Adaptor is also available for use with bisulfite sequencing protocols (NEB #E7535).
For larger volume requirements, customized and bulk packaging is available by purchasing through the OEM/Bulks department at NEB. Please contact OEM@neb.com for further information.
The following reagents are supplied with this product:
Store at (°C) Concentration NEBNext Adaptor for Illumina -20 USER® Enzyme -20 NEBNext® i509 Primer NEBNext® i510 Primer NEBNext® i511 Primer NEBNext® i512 Primer NEBNext® i513 Primer NEBNext® i514 Primer NEBNext® i515 Primer NEBNext® i516 Primer NEBNext® i713 Primer NEBNext® i714 Primer NEBNext® i715 Primer NEBNext® i716 Primer NEBNext® i717 Primer NEBNext® i718 Primer NEBNext® i719 Primer NEBNext® i720 Primer NEBNext® i721 Primer NEBNext® i722 Primer NEBNext® i723 Primer NEBNext® i724 Primer
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- Adaptors and Primers for Library Prep,
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- FFPE DNA ,
- DNA Library Prep ,
- Microbiome DNA Enrichment ,
- RNA Library Prep
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