NEBNext® Multiplex Oligos Selection Chart

 

SINGLE INDEX

DUAL INDEX

UNIQUE DUAL INDEX

UNIQUE DUAL INDEX UMIs

NEBNext Multiplex Oligos for Illumina®

Contains UMI

No

No

No

Yes

Addresses index hopping

No

No

Yes

Yes

Indexing Strategy

Index Primer

Index Primer

Index Primer

Index Adaptor

Adaptor Included
NEBNext Adaptor (loop) NEBNext Adaptor (loop) Standard: NEBNext Adaptor (loop)
LV: None; adaptor provided with library prep kit
Unique Dual Index UMI Adaptor

Applications

DNA-seq, RNA-seq (except 1st-gen small RNA)

DNA-seq, RNA-seq (except 1st-gen small RNA)

DNA-seq, RNA-seq (except 1st-gen small RNA), Low-bias Small RNA

PCR-free DNA-seq, DNA-seq, RNA-seq (except 1st-gen small RNA)

Number of indices for multiplexing

up to 144

up to 384

up to 480

384 for DNA; 96 for RNA

Compatible with ARTIC sequencing for Illumina
Yes Yes Yes No

Compatible with EM-seq

Yes*

Yes*

Yes*

No

Number of sets available; formats and indices available

Five; Sets 1-4 (12 indices/set): Individual vials; 96 Index: Premixed plate

Two; individual vials containing 8 i5 primers and 12 i7 primers for combinatorial mixing

Twelve; Sets are provided as either 24 or 96 indices in premixed, foil-sealed 96-well plates; NEBNext LV Unique Dual Index Primers are meant for use with certain workflows, including EM-seq & EM-seq v2, Low-bias Small RNA, and Eh5mC-seq

Four sets for DNA, One set for RNA; Adaptors with 96 indices in premixed, foil-sealed 96-well plate (DNA-seq or RNA-seq) and primers

* Requires use of the EM-seq Adaptor. Index primers for use with these kits are available separately (NEBNext LV Unique Dual Index Primer Pairs Sets 1-5).