Home Resources Selection Charts NEBNext^® Multiplex Oligos Selection Chart

NEBNext^® Multiplex Oligos Selection Chart

	SINGLE INDEX	DUAL INDEX	UNIQUE DUAL INDEX	UNIQUE DUAL INDEX UMIs
NEBNext Multiplex Oligos for Illumina^®	Index Primers Set 1 Index Primers Set 2 Index Primers Set 3 Index Primers Set 4 96 Index Primers	Dual Index Primers Set 1 Dual Index Primers Set 2	96 Unique Dual Index Primer Pairs Set 1 96 Unique Dual Index Primer Pairs Set 2 96 Unique Dual Index Primer Pairs Set 3 96 Unique Dual Index Primer Pairs Set 4 96 Unique Dual Index Primer Pairs Set 5 LV Unique Dual Index Primers Set 1 LV Unique Dual Index Primers Set 2 LV Unique Dual Index Primers Set 2A LV Unique Dual Index Primers Set 2B LV Unique Dual Index Primers Set 3 LV Unique Dual Index Primers Set 4 LV Unique Dual Index Primers Set 5	Unique Dual Index UMI Adaptors DNA Set 1 Unique Dual Index UMI Adaptors DNA Set 2 Unique Dual Index UMI Adaptors DNA Set 3 Unique Dual Index UMI Adaptors DNA Set 4 Unique Dual Index UMI Adaptors RNA Set 1
Contains UMI	No	No	No	Yes
Addresses index hopping	No	No	Yes	Yes
Indexing Strategy	Index Primer	Index Primer	Index Primer	Index Adaptor
Adaptor Included	NEBNext Adaptor (loop)	NEBNext Adaptor (loop)	Standard: NEBNext Adaptor (loop) LV: None; adaptor provided with library prep kit	Unique Dual Index UMI Adaptor
Applications	DNA-seq, RNA-seq (except 1^st-gen small RNA)	DNA-seq, RNA-seq (except 1^st-gen small RNA)	DNA-seq, RNA-seq (except 1^st-gen small RNA), Low-bias Small RNA	PCR-free DNA-seq, DNA-seq, RNA-seq (except 1^st-gen small RNA)
Number of indices for multiplexing	up to 144	up to 384	up to 480	384 for DNA; 96 for RNA
Compatible with ARTIC sequencing for Illumina	Yes	Yes	Yes	No
Compatible with EM-seq	Yes*	Yes*	Yes*	No
Number of sets available; formats and indices available	Five; Sets 1-4 (12 indices/set): Individual vials; 96 Index: Premixed plate	Two; individual vials containing 8 i5 primers and 12 i7 primers for combinatorial mixing	Twelve; Sets are provided as either 24 or 96 indices in premixed, foil-sealed 96-well plates; NEBNext LV Unique Dual Index Primers are meant for use with certain workflows, including EM-seq & EM-seq v2, Low-bias Small RNA, and Eh5mC-seq	Four sets for DNA, One set for RNA; Adaptors with 96 indices in premixed, foil-sealed 96-well plate (DNA-seq or RNA-seq) and primers

* Requires use of the EM-seq Adaptor. Index primers for use with these kits are available separately (NEBNext LV Unique Dual Index Primer Pairs Sets 1-5).

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