Epigenetics
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  • Epigenetics

    Epigenetics is the study of heritable changes in gene expression that are not encoded in the DNA of the genome. Encouraging evidence has linked epigenetic effects to oncogenesis, progression and treatment of cancer (1), the regulation of development and function of the nervous system (2), gene regulation (3), cellular stress events (3), nutrigenomics (4), aging and DNA repair (5). Considerable ongoing efforts are directed towards identifying the dynamic functions of various modifications to DNA and its associated proteins and elucidating their mechanisms.

    References

    1. Baylin, S. and Jones, P. (2011) Nature Rev Cancer (10), 726-734. PMID: 21941284 
    2. Riccio, A., (2010) Nature Neuroscience, 13, 1330-1337. PMID: 20975757 
    3. Huang, J. et al. (2006) Nature, 444, 629-632. PMID: 17108971 
    4. Park, L.K., Friso, S. and Choi, S.W. (2011) Proc. Nutr. Soc. Nov 4, 1-9. 
    5. Pahlich, S., Zakaryan, R.P. and Gehring., H. (2006) Biochim, Biophys. Acta. 1764, 1890-1903. PMID: 17010682
    1. What Is Epigenetics?

      If all cells are created from the same genetic material, why are there so many different cell types? Listen to Sriharsa Pradhan, Senior Scientist, RNA Biology at NEB, as he describes how DNA is methylated and how this affects the path of reading the DNA code the same way an obstruction would derail a train off its tracks.

    2. Interactive Tutorial Explaining the Phenomenon of Epigenetics at a Molecular Level

      Watch an interactive tutorial explaining the different molecular mechanisms by which epigenetic change influences gene expression. Learn about how NEB’s line of EpiMark® validated reagents are targeted to the various enzymes and DNA elements that are altered by epigenetic change.

    Epigenetics includes these areas of focus:

    Restriction Enzymes for Epigenetics
    DNA Methylation Analysis
    5-hmC Detection & Analysis
    Methylated DNA Analysis
    Bisulfite Conversion
    Histones & Nucleosome Assembly
    DNA Enrichment
    Antibodies
    Control DNA for Epigenetics
    ChIP
    Histone Methyltransferases

    FAQs for Epigenetics

      Publications related to Epigenetics:

    1. Marx V. (2016). Genetics: profiling DNA methylation and beyond Nature Methods. 13, 119-122. DOI: 10.1038/nmeth.3736
    2. Estève P., Zhang G., Ponnaluri V., Kanneganti (2015). Binding of 14-3-3 reader proteins to phosphorylated DNMT1 facilitates Nucleic Acids Res. PubMedID: 26553800, DOI: 10.1093/nar/gkv1162
    3. Kienhöfer S., Musheev M., Stapf U., Helm M., Schomacher L., Niehrs C., Schäfer A. (2015). GADD45a physically and functionally interacts with TET1Publication Differentiation. PubMedID: 26546041, DOI: 10.1016/j.diff.2015.10.003
    4. Page A., Paoli P., Salvador E., White S., French J., Mann J. (2015). Hepatic Stellate Cell Transdifferentiation Involves Genome-Wide Remodeling of the DNA Methylation Landscape J Hepatol. PubMedID: 26632634, DOI: 10.1016/j.jhep.2015.11.024
    5. Wee E., Ngo T., Trau M. (2015). A simple bridging flocculation assay for rapid, sensitive and stringent detection of gene specific DNA methylation Sci Rep. 5, 15028. PubMedID: 26458746, DOI: 10.1038/srep15028
    6. Chernov AV., Reyes L., Peterson S., Strongin AY. (2015). Depletion of CG-Specific Methylation in Mycoplasma hyorhinis Genomic DNA after Host Cell Invasion PLoS One. 10, e0142529. PubMedID: 26544880, DOI: 10.1371/journal.pone.0142529
    7. Chandra S, Baribault C, Lacey M, Ehrlich M (2014). Myogenic differential methylation: diverse associations with chromatin structure Biology (Basel). 3(2), 426-51. PubMedID: 24949935, DOI: 10.3390/biology3020426
    8. Hughes J.R., Roberts N., McGowan S., Hay D., Giannoulatou E., Lynch M., De Gobbi M., Taylor S., Gibbons R., Higgs D.R. (2014). Analysis of hundreds of cis-regulatory landscapes at high resolution in a single, high-throughput experiment Nat Genet. 46 (2), 205-212. PubMedID: 24413732, DOI: doi:10.1038/ng.2871
    9. Sexton T, Kurukuti S, Mitchell JA, Umlauf D, Nagano T, Fraser P (2012). Sensitive detection of chromatin coassociations using enhanced chromosome conformation capture on chip Nat Protoc. 7(7), 1335-50. PubMedID: 22722369, DOI: 10.1038/nprot.2012.071
    10. Wolff, E.M. et al. (2011). Hypomethylation of a LINE-1 Promoter Activates an Alternate Transcript of the MET Oncogene in Bladders with Cancer PLoS Genet. . 6, 4:e1000917. PubMedID: 20421991, DOI: 10.1371/journal.pgen.1000917
    11. Lieberman-Aiden E., van Berkum N., Williams L., Imakaev M., Ragoczy T., Telling A., Amit I., Lajoie B., Sabo P., Dorschner M., Sandstrom R., Bernstein B., BenderM. A., Groudine M., Gnirke A., Stamatoyannopoulos J., Mirny L., Lander E., Dekker J. (2009). Comprehensive mapping of long range interactions reveals folding principles of the human genome Science. Oct 9; 326(5950), 289–293.