DNA Methylation Analysis
DNA methylation plays a pivotal role in development and disease in eukaryotes and regulates phenotypes in prokaryotes. Aberrant methylation profiles that characterize diseases have potential for biomarkers and precise epigenomic therapies.
NEB offers a range of solutions for investigating DNA methylation. Enzymatic conversion is highly recommended over conventional bisulfite conversion to generate more accurate and sensitive 5mC results with next generation sequencing or PCR. The EpiMark® and NEBNext® kits simplify detection, amplification, and mapping of methylated or hydroxymethylated cytosine with enzymatic conversion modules, next generation sequencing library preparation kits with enzymatic conversion integrated, and validated primer sets for multiplexing. If conventional bisulfite conversion is desired, optimized DNA polymerases, high yielding library preparation kits, and targeted sequencing techniques are useful to address challenges for amplification and sequencing. Enrichment kits using high affinity MBD2-Fc fusion protein and magnetic bead capture of methylated CpGs from genomic DNA offer a cost-effective approach for genome-wide DNA methylation studies.
- Enzymatic Conversion for DNA Methylation Analysis
- Hydroxymethylation Detection and Analysis
- Methylome Analysis
- Enrichment for Methylated DNA (5mC) and RNA (m6A)
- Methylation-Sensitive and Methylation-Dependent Restriction Enzymes for Epigenetics
- Analysis of DNA Methylation by Bisulfite Conversion
Choose Type:
- How should EM-seq sequencing data be analyzed?
- How many indices are available with the 24 reaction or 96 reaction size of the NEBNext® Primers for Epigenetics?
- Which DNA polymerase do you recommend for amplification of converted DNA?
- What types of epigenetic modifications can be identified using the Nucleoside Digestion Mix?
- Can I use the NEBNext UltraExpress® FS DNA Library Prep Kit for bisulfite conversion and EM-seq™ workflows?
- Comparison of Methods for DNA Methylation Analysis
- Restriction Enzymes for Epigenetics Selection Chart
Selection Tools
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This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.
