epigenetics banner

DNA Methylation Analysis

DNA methylation plays a pivotal role in development and disease in eukaryotes and regulates phenotypes in prokaryotes. Aberrant methylation profiles that characterize diseases have potential for biomarkers and precise epigenomic therapies.

NEB offers a range of solutions for investigating DNA methylation. Enzymatic conversion is highly recommended over conventional bisulfite conversion to generate more accurate and sensitive 5mC results with next generation sequencing or PCR. The EpiMark® and NEBNext® kits simplify detection, amplification, and mapping of methylated or hydroxymethylated cytosine with enzymatic conversion modules, next generation sequencing library preparation kits with enzymatic conversion integrated, and validated primer sets for multiplexing. If conventional bisulfite conversion is desired, optimized DNA polymerases, high yielding library preparation kits, and targeted sequencing techniques are useful to address challenges for amplification and sequencing. Enrichment kits using high affinity MBD2-Fc fusion protein and magnetic bead capture of methylated CpGs from genomic DNA offer a cost-effective approach for genome-wide DNA methylation studies.  



DNA methylation analysis for research and development

DNA methylation analysis is useful for research or diagnostics development for cancer, other human diseases, eDNA, plants, and prokaryotic epigenomes



 


Choose Type:

DNA Methylation Analysis includes these areas of focus:
Hydroxymethylation Detection and Analysis
Analysis of DNA Methylation by Bisulfite Conversion
Enrichment for Methylated DNA (5mC) and RNA (m6A)
Enzymatic Conversion for DNA Methylation Analysis
Methylation Sensitive and Methylation Dependent Restriction Enzymes for Epigenetics
FAQs for DNA Methylation Analysis
Protocols for DNA Methylation Analysis
Application Notes for DNA Methylation Analysis
Legal Information

Products and content are covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB). The use of trademark symbols does not necessarily indicate that the name is trademarked in the country where it is being read; it indicates where the content was originally developed. The use of this product may require the buyer to obtain additional third-party intellectual property rights for certain applications. For more information, please email busdev@neb.com.

This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.


Videos

  • Epigenetics_Tutorial_TN

    Interactive Tutorial Explaining the Phenomenon of Epigenetics at a Molecular Level

    Watch an interactive tutorial explaining the different molecular mechanisms by which epigenetic change influences gene expression. Learn about how NEB’s reagents are targeted to the various enzymes and DNA elements that are altered by epigenetic change.

  • NEBTV_Ep-28_thumbnail_1920_1

    NEB® TV Ep. 28 – Advances in Methylome Analysis

    In this episode, we discuss the challenges associated with current methods available for methylome analysis, and introduce NEBNext Enzymatic Methyl-seq (EM-seq™) as an alternative to bisulfite sequencing that addresses these challenges.

  • What Does Epigenetics Have to Do with Honeybees?

    What Does Epigenetics Have to Do with Honeybees?

    Rick Feehery, Research Associate at New England Biolabs, Inc, explains the mechanisms of epigenetic DNA modification. To drive home the power of such tiny changes, he uses the example of honeybee colonies, wherein DNA methylation is the only factor that determines whether a bee is born a worker, drone or queen.

Feature Articles