NEBNext® Multiplex Oligos for Enzymatic Methyl-seq (Unique Dual Index Primer Pairs)
This product has been discontinued as of June 16, 2025. As an alternative to the EM-seq™ Primer Pairs, users can purchase the NEBNext LV Unique Dual Index Primer Pairs (Sets 1-5) (NEB #E3400, E3402, E3404, E3406, E3408, E3390, E3392). The EM-seq Adaptor will be available in the NEBNext Enzymatic Methyl-seq Kit ( NEB #E7120). This change does not impact users of NEBNext Enzymatic Methyl-seq v2 Kit and/or Conversion Module.
- Replaced by NEBNext® LV Unique Dual Index Primers Set 1 on June 16, 2025
- Catalog # E7140 was discontinued on June 16, 2025
NEBNext Multiplex Oligos for Enzymatic Methyl-seq (Unique Dual Index Primer Pairs) is compatible with Illumina® sequencing and includes the NEBNext EM-seq™ Adaptor, and NEBNext PCR primers that enable incorporation of unique index pairs during library amplification. The index primers are provided as pre-mixed unique pairs of i5 and i7 index primers, packaged in a single-use 96-well plate with a pierceable foil seal, or in individual vials.
Although optimized for the EM-seq workflow, the NEBNext EM-seq Adaptor is also compatible with bisulfite sequencing workflows.
- Optimized for the EM-seq workflow
- Compatible with bisulfite sequencing workflows
- Addresses the “barcode hopping” issue seen with some Illumina sequencing instruments
- Increases sample identification specificity with unique dual index primer pairs
Storage temperature: -20°C.
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Product Information
NEBNext Multiplex Oligos for Enzymatic Methyl-seq (Unique Dual Index Primer Pairs) is compatible with Illumina® sequencing and includes the NEBNext EM-seq™ Adaptor, and NEBNext PCR primers that enable incorporation of unique index pairs during library amplification.
The 96-reaction size includes a single-use 96-well plate with a pierceable foil seal. Each well of the plate contains a unique pair of one i5 index primer and one i7 index primer, therefore providing 96 unique dual indices. For the 24-reaction size, indices are provided in 24 separate vials, with each vial containing a unique pair of one i5 index primer and one i7 index primer. There is no overlap between i5 and i7 index pairs provided in the 24 and 96 reaction kits.
Although optimized for the EM-seq workflow, the NEBNext EM-seq Adaptor is also compatible with bisulfite sequencing workflows.
- This product is related to the following categories:
- Discontinued (<3 years)
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