EcoGII Methyltransferase

The following reagents are supplied with this product:

NEB #	Component Name	Component #	Stored at (°C)	Amount	Concentration
M0603S     -20       EcoGII Methyltransferase M0603SVIAL -20 1 x 0.04 ml 5,000 units/ml   rCutSmart™ Buffer B6004SVIAL -20 1 x 1.25 ml 10 X   S-adenosylmethionine (SAM) B9003SVIAL -20 1 x 0.1 ml 32 mM

Companion Products

• S-adenosylmethionine (SAM)

1. Storage of SAM: S-adenosylmethionine (Sigma Catalog #A7007) is stored at –20°C as a 32 mM solution dissolved in 0.005 M sulfuric acid and 10% ethanol. Under these conditions SAM is stable for up to 6 months. SAM is unstable at 37°C (pH 7.5)(2), and should be replenished in reactions incubated longer than 4 hours. 
2. For use of methylation reaction the SAM should be diluted 1:200 in H₂O to a final concentration of 160 µM.
3. EcoGII Methyltransferase is sensitive to salt. Make sure the DNA solution is low in salt concentration or that it makes up only a small percentage of the final reaction volume. If salt is a problem, reduce the salt concentration by drop dialysis. 

1. Fang, et al (2012). Nature Biotechnology. 30, 1232-1239.
2. Hoffman, J.L. (1986). Biochemistry. 25, 4444-4449.

1. Recommended Protocol for ³H labeling of DNA
2. Recommended Protocol for Methylation of Genomic DNA

1. Will any NEB methyltransferases (methylases) work on RNA?
2. What is the activity of EcoGII Methyltransferase at temperatures other than 37°C?
3. Is S-adenosylmethionine (SAM) supplied with the Methyltransferase?
4. Does EcoGII Methyltransferase require BSA?
5. Does EcoGII Methyltransferase require MgCl2?
6. What is the activity of EcoGII Methyltransferase in other buffers?
7. What is the molecular weight of EcoGII Methyltransferase?
8. Can EcoGII Methyltransferase be heat inactivated?
9. Is EcoGII Methyltransferase sensitive to salt?
10. Will EcoGII Methyltransferase methylate ssDNA?
11. Will EcoGII Methyltransferase methylate DNA/RNA hybrids?
12. Will EcoGII Methyltransferase methylate gDNA?
13. Will EcoGII Methyltransferase methylate RNA?
14. Will EcoGII methylation block restriction enzymes? 
15. How can I increase methylation of my substrate by EcoGII Methyltransferase?
16. Can I purchase large amounts of an existing Enzyme for Innovation?
17. What are Enzymes for Innovation?
18. Can you tell me more about the switch from BSA to Recombinant Albumin (rAlbumin) in NEBuffers?

Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.

The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]

• M0603S_L_v1
• M0603S_L_v2
• M0603S_L_v3

The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]

• M0603S_L_v1_0031702
• M0603S_L_v1_0031712
• M0603S_L_v1_0031803
• M0603S_v1_10012609
• M0603S_v1_10027768
• M0603S_v1_10039975
• M0603S_v1_10052721
• M0603S_v1_10070386
• M0603S_v1_10092945
• M0603S_v1_10115733
• M0603S_v1_10143375
• M0603S_v1_10156257
• M0603S_v1_10163863
• M0603S_v1_10185491
• M0603S_v1_10216383
• M0603S_v2_10236802
• M0603S_v2_10246677
• M0603S_v3_10253399
• M0603S_v3_10263575

The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.

• EcoGII Methyltransferase
• rCutSmart™ Buffer
• S-adenosylmethionine (SAM)

Products and content are covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB). The use of trademark symbols does not necessarily indicate that the name is trademarked in the country where it is being read; it indicates where the content was originally developed. The use of this product may require the buyer to obtain additional third-party intellectual property rights for certain applications. For more information, please email busdev@neb.com.

This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

New England Biolabs (NEB) is committed to practicing ethical science – we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner. Learn more.