neb31 cloned at NEB recombinant engineered time saver dil_B 37 65 Heat reduced star activity

SspI-HF has been reformulated with Recombinant Albumin (rAlbumin) beginning with Lot #10141296. Learn more.

We are excited to announce that all reaction buffers are now BSA-free. NEB began switching our BSA-containing reaction buffers in April 2021 to buffers containing Recombinant Albumin (rAlbumin) for restriction enzymes and some DNA modifying enzymes. Find more details at

NEB high fidelity restriction endonuclease that recognizes the sequence AAT_^ATT

High-Fidelity (HF®) restriction enzymes have the same specificity as native enzymes, but have been engineered for significantly reduced star activity and performance in a single buffer (rCutSmart Buffer).  All HF-restriction enzymes come with Gel Loading Dye, Purple (6X) .  Enjoy the enhanced performance and added value of our engineered enzymes at the same price as the native enzyme:

  • Engineered for improved performance
  • 100% activity in rCutSmart Buffer
  • Time-Saver™ qualified for digestion in 5-15 minutes
  • Reduced star activity
  • Supplied with 1 vial of Gel Loading Dye, Purple (6X)
  • Restriction Enzyme Cut Site: AAT/ATT
Catalog # Concentration Size List Price Your Price Quantity
R3132S 20,000 units/ml 1,000 units $80.00
R3132L 20,000 units/ml 5,000 units $333.00
R3132M 100,000 units/ml 5,000 units $333.00
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