I-SceI

Isoschizomers | Single Letter Code | Pronunciation:

Having supplied restriction enzymes to the research community for over 40 years, NEB has earned the reputation of being the leader in enzyme technologies. Working continuously to be worthy of that distinction, NEB strives to develop enzyme of the highest purity and unparalleled quality.

100% activity in CutSmart^® Buffer (over 210 enzymes are available in the same buffer) allowing for easier double digests
This is a homing endonuclease
Tolerates some sequence degeneracy within recognition sequence

Catalog #	Concentration	Size	List Price	Your Price
R0694S	5,000 units/ml	500 units	$72.00	Sign In Sign In
R0694L	5,000 units/ml	2,500 units	$296.00	Sign In Sign In

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Product Information

I-SceI is an intron-encoded endonuclease. The intron encoding I-SceI is present in the mitochondria of Saccharomyces cerevisiae.

Product Source

An E. coli strain that carries the I-SceI mitochondrial gene from Saccharomyces cerevisiae (B. Dujon).

Reagents Supplied

The following reagents are supplied with this product:

Show all

NEB # Component Name Component # Stored at (°C) Amount Concentration

R0694S

-80

I-SceI	R0694SVIAL	-80	1 x 0.1 ml	5,000 units/ml
CutSmart® Buffer	B7204SVIAL	-20	1 x 1.25 ml	10 X
pGPS2 NotI-linearized Control Plasmid	N0420SVIAL	-20	1 x 0.01 ml	0.5 mg/ml

R0694L

-80

I-SceI	R0694LVIAL	-80	1 x 0.5 ml	5,000 units/ml
CutSmart® Buffer	B7204SVIAL	-20	1 x 1.25 ml	10 X
pGPS2 NotI-linearized Control Plasmid	N0420SVIAL	-20	1 x 0.01 ml	0.5 mg/ml

Product Categories:: Homing Endonucleases Products,; Restriction Endonucleases H M

Applications:: Restriction Enzyme Digestion

Properties & Usage
Unit Definition
One unit is defined as the amount of enzyme required to cleave 1 µg of pGPS2 NotI-Iinearized Control Plasmid in 1 hour at 37°C in a total reaction volume of 50 µl.
Reaction Conditions

1X CutSmart® Buffer
Incubate at 37°C

1X CutSmart® Buffer
50 mM Potassium Acetate
20 mM Tris-acetate
10 mM Magnesium Acetate
100 µg/ml BSA
(pH 7.9 @ 25°C)

Activity in NEBuffers
NEBuffer™ 1.1: 10%
NEBuffer™ 2.1: 50%
NEBuffer™ 3.1: 25%
CutSmart® Buffer: 100%

Diluent Compatibility
- Diluent B
Storage Conditions

10 mM Tris-HCl
300 mM NaCl
1 mM DTT
0.1 mM EDTA
500 µg/ml BSA
50% Glycerol
pH 7.4 @ 25°C

Heat Inactivation
65°C for 20 min
Related Products
Companion Products
Product Notes
1. Homing endonucleases do not have stringently-defined recognition sequences in the way that restriction enzymes do. That is, single base changes do not abolish cleavage but reduce its efficiency to variable extents. The precise boundary of required bases is generally not known. The recognition sequence listed is one site that is known to be recognized and cleaved.
2. Supplied with plasmid DNA. NotI-linearized pGPS2 is supplied at 0.5 mg/ml in 10 mM Tris-HCl (pH 8.0), 1 mM EDTA. Cleavage of this 2499 bp plasmid with I-SceI gives fragments of 1518 and 981 base pairs.
3. The enzyme retains full activity after at least 10 free-thaw cycles; the enzyme can be aliquoted and stored at –80C.

Protocols, Manuals & Usage
- Protocols
  Optimizing Restriction Endonuclease Reactions
  
  Double Digest Protocol with Standard Restriction Enzymes
  
  Restriction Digest Protocol
- Usage Guidelines
  Activity at 37°C for Restriction Enzymes with Alternate Incubation Temperatures
  
  Activity of Restriction Enzymes in PCR Buffers
  
  Cleavage Close to the End of DNA Fragments
  
  Digestion of Agarose-Embedded DNA: Info for Specific Enzymes
  
  Double Digests
  
  Heat Inactivation
  
  NEBuffer Activity/Performance Chart with Restriction Enzymes
  
  Optimizing Restriction Endonuclease Reactions
  
  Restriction Endonucleases - Survival in a Reaction
  
  Restriction Enzyme Diluent Buffer Compatibility
  
  Restriction Enzyme Tips
  
  Single Letter Codes
  
  Star Activity
  
  Traditional Cloning Quick Guide
Tools & Resources
- Selection Charts
  Alphabetized List of Recognition Specificities
  
  Cross Index of Recognition Sequences
  
  Dam-Dcm and CpG Methylation
  
  Enzymes with Nonpalindromic Sequences
  
  Frequencies of Restriction Sites
  
  Isoschizomers
  
  NEB Diluent and Buffer Table
  
  Why Choose Recombinant Enzymes?
- Web Tools
  Competitor Cross-Reference Tool
  
  DNA Sequences and Maps Tool
  
  Double Digest Finder
  
  Enzyme Finder
  
  NEBcutter V2.0
  
  NEBioCalculator
  
  REBASE
FAQs & Troubleshooting
- FAQs
  I tested your restriction enzyme on the substrate DNA recommended by NEB, and it appears to be active, however it does not digest my DNA. What could be the reason?
  
  Why is my Restriction Enzyme not cutting DNA?
  
  Why do I see a DNA smear on an agarose gel after a restriction digest?
  
  Why do I see additional DNA bands on my gel after a restriction digest?
  
  How many nucleotides do I have to add adjacent to the RE recognition site in order to get efficient cutting?
- Troubleshooting
  Restriction Enzyme Troubleshooting Guide
Citations & Technical Literature
- Citations
  
  Product Citation Tool
  
  Powered by Bioz See more details on Bioz
Quality, Safety & Legal
- Quality Control Assays
  
  Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.
- Specifications
  The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]
  
  R0694S_L_v1
- Certificate of Analysis
  The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]
  
  R0694S_L_v1_0121405
  
  R0694S_L_v1_0111302
  
  R0694S_L_v1_0111306
  
  R0694S_L_v1_0111310
  
  R0694S_L_v1_0111312
  
  R0694S_L_v1_0121411
  
  R0694S_L_v1_0121504
  
  R0694S_L_v1_0131504
  
  R0694S_L_v1_0131509
  
  R0694S_L_v1_0131605
  
  R0694S_L_v1_0141605
  
  R0694S_L_v1_0201804
  
  R0694L_v1_10008634
  
  R0694L_v1_10022297
  
  R0694S_v1_10030587
  
  R0694S_v0_10037161
  
  R0694L_v1_10043034
  
  R0694S_v1_10050598
  
  R0694S_v1_10061114
  
  R0694L_v1_10061113
  
  R0694S_v1_10072122
  
  R0694L_v1_10079891
  
  R0694S_v1_10081460
  
  R0694L_v1_10084444
  
  R0694L_v1_10091233
  
  R0694S_v1_10091197
  
  R0694S_v1_10092658
- Safety Data Sheets
  The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.
  
  I-SceI
  
  CutSmart® Buffer
  
  pGPS2 NotI-linearized Control Plasmid
- Legal and Disclaimers
  
  This product is covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB).
  
  While NEB develops and validates its products for various applications, the use of this product may require the buyer to obtain additional third party intellectual property rights for certain applications.
  
  For more information about commercial rights, please contact NEB's Global Business Development team at [email protected].
  
  This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.
  
  New England Biolabs (NEB) is committed to practicing ethical science – we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner. Learn more.