I-SceI

Having supplied restriction enzymes to the research community for over 40 years, NEB has earned the reputation of being the leader in enzyme technologies. Working continuously to be worth of that distinction, NEB strives to develop enzyme of the highest purity and unparalleled quality.
- 100% activity in CutSmart® Buffer (over 210 enzymes are available in the same buffer) allowing for easier double digests
- This is a homing endonuclease
- Tolerates some sequence degeneracy within recognition sequence
Featured Videos
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Reduce Star Activity with High-Fidelity Restriction Enzymes
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Standard Protocol for Restriction Enzyme Digests
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NEB TV Ep. 15 – Applications of Restriction Enzymes
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CutSmart Restriction Enzyme Buffer
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Restriction Enzyme Digest Protocol: Cutting Close to DNA End
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Restriction Enzyme Digestion Problem: DNA Smear on Agarose Gel
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Why is My Restriction Enzyme Not Cutting DNA?
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Restriction Enzyme Digest Problem: Too Many DNA Bands
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Double Digestion with NEBcloner
Ordering Information

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Product Information
I-SceI is an intron-encoded endonuclease. The intron encoding I-SceI is present in the mitochondria of Saccharomyces cerevisiae. Product Source
An E. coli strain that carries the I-SceI mitochondrial gene from Saccharomyces cerevisiae (B. Dujon).Reagents Supplied
The following reagents are supplied with this product:
Store at (°C) Concentration CutSmart® Buffer -20 10 X pGPS2 NotI-linearized Control Plasmid -80 500 μg/ml - Product Categories:
- Homing Endonucleases Products,
- Restriction Endonucleases H M
- Applications:
- Restriction Enzyme Digestion
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FAQs & Tech Tips
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Protocols & Manuals
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Other Tools & Resources
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Quality & Safety
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Legal Information
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