Home Restriction Endonucleases Products Nb.BsrDI

Nb.BsrDI

incubation temp

heat inactivation

NbBsrDI_cutsite

Isoschizomers | Single Letter Code

Having supplied restriction enzymes to the research community for over 40 years, NEB has earned the reputation of being the leader in enzyme technologies. Working continuously to be worth of that distinction, NEB strives to develop enzyme of the highest purity and unparalleled quality.

100% activity in CutSmart^® Buffer (over 210 enzymes are available in the same buffer) allowing for easier double digests
This is a nicking endonuclease
Generates DNA molecules that are “nicked”, rather than cleaved

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Ordering Information

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Product Information
Nb.BsrDI is a nicking endonuclease that cleaves only one strand of DNA on a double-stranded DNA substrate.
Product Source
An E. coli strain expressing only the large subunit of the BsrDI restriction gene from Bacillus stearothermophilus D70 (Z. Chen).
Reagents Supplied

The following reagents are supplied with this product:

Store at (°C) Concentration

CutSmart® Buffer -20 10 X

Product Categories:

Nicking Endonucleases Products,

Restriction Endonucleases: N-O
- Properties and Usage
  Unit Definition
  One unit is defined as the amount of enzyme required to convert 1 µg of supercoiled pUC19 DNA to open circular form in 1 hour at 65°C in a total reaction volume of 50 µl.
  Reaction Conditions
  
  1X CutSmart® Buffer
  Incubate at 65°C
  
  1X CutSmart® Buffer
  50 mM Potassium Acetate
  20 mM Tris-acetate
  10 mM Magnesium Acetate
  100 μg/ml BSA
  (pH 7.9 @ 25°C)
  
  Activity in NEBuffers
  CutSmart® Buffer: 100%
  NEBuffer 1.1: 25%
  NEBuffer 2.1: 100%
  NEBuffer 3.1: 100%
  
  Diluent Compatibility
  
  Diluent A
  
  Storage Temperature
  -20°C
  Storage Conditions
  
  10 mM Tris-HCl
  200 mM NaCl
  1 mM DTT
  0.1 mM EDTA
  50% Glycerol
  200 μg/ml BSA
  pH 7.4 @ 25°C
  
  Heat Inactivation
  80°C for 20 min
  Methylation Sensitivity
  
  dam methylation: Not Sensitive
  dcm methylation: Not Sensitive
  CpG Methylation: Not Sensitive
  
  Activity at Temperature
  @37°C: 75%
- Related Products
  Companion Products
  
  Monarch® Plasmid Miniprep Kit
  
  Monarch® DNA Gel Extraction Kit
  
  Monarch® PCR & DNA Cleanup Kit (5 μg)
  
  Materials Sold Separately
  
  CutSmart® Buffer
- Notes
  To see how this enzyme (along with other nicking enzymes) performs at different temperatures, please visit Effect of Various Temperatures of Different Nicking Endonucleases.
- References
  Song, Q. et al. (2010). Anal. Chem. [Epub ahead of print].
  
  Zhang, P. et al. (2010). Protein Expr. Purif. 69, 226-234. [Epub 2009 Sep 9].
FAQs & Tech Tips
- FAQs
  My enzyme used to come with another NEBuffer, but CutSmart™ Buffer is now recommended? Why?
  
  How can I access the old NEBuffer Activity Chart?
  
  Why is my Restriction Enzyme not cutting DNA?
  
  Why do I see a DNA smear on an agarose gel after a restriction digest?
  
  Why do I see additional DNA bands on my gel after a restriction digest?
  
  How many nucleotides do I have to add adjacent to the RE recognition site in order to get efficient cutting?
Protocols & Manuals
- Protocols
  Optimizing Restriction Endonuclease Reactions
  
  Double Digest Protocol with Standard Restriction Enzymes
  
  Restriction Digest Protocol
Other Tools & Resources
Quality & Safety
- Quality Control Assays
  
  Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.
- Specifications
  The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]
  
  R0648S_L_v1
- Certificate of Analysis
  The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]
  
  R0648S_L_v1_0041302
  
  R0648S_L_v1_0041312
  
  R0648S_L_v1_0041408
  
  R0648S_L_v1_0041503
  
  R0648S_L_v1_0041507
  
  R0648S_L_v1_0041601
  
  R0648S_L_v1_0041602
  
  R0648S_L_v1_0041606
  
  R0648S_L_v1_0041611
  
  R0648S_L_v1_0041708
  
  R0648S_L_v1_0071803
  
  R0648S_v1_10019050
  
  R0648L_v1_10033903
- Safety Data Sheets
  The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.
  
  Nb.BsrDI
  
  CutSmart® Buffer
Legal Information
- Legal And Disclaimers
  
  This product is covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB).
  
  While NEB develops and validates its products for various applications, the use of this product may require the buyer to obtain additional third party intellectual property rights for certain applications.
  
  For more information about commercial rights, please contact NEB's Global Business Development team at [email protected].
  
  This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

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