Nb.BsrDI

We are excited to announce that we are in the process of switching all reaction buffers to be BSA-free. Beginning April 2021, we will be gradually transitioning to buffers containing Recombinant Albumin (rAlbumin) for restriction enzymes and some DNA modifying enzymes. All information on the website has been updated to reflect this change. Find more details at www.neb.com/BSA-free.

NEB restriction endonuclease that recognizes the sequence GCAATG_NN

Isoschizomers | Single Letter Code | Pronunciation:

Having supplied restriction enzymes to the research community for over 45 years, NEB has earned the reputation of being the leader in enzyme technologies. Working continuously to be worthy of that distinction, NEB strives to develop enzyme of the highest purity and unparalleled quality.

100% activity in rCutSmart^™ Buffer (over 215 enzymes are available in the same buffer) allowing for easier double digests
This is a nicking endonuclease
Generates DNA molecules that are “nicked”, rather than cleaved
Cut Site: GCAATG

Catalog #	Concentration	Size	List Price	Your Price
R0648S	10,000 units/ml	1,000 units	$72.00	Sign In Sign In
R0648L	10,000 units/ml	5,000 units	$290.00	Sign In Sign In

Please enter a quantity for at least one size

Product Information

Nb.BsrDI is a nicking endonuclease that cleaves only one strand of DNA on a double-stranded DNA substrate.

Product Source

An E. coli strain expressing only the large subunit of the BsrDI restriction gene from Bacillus stearothermophilus D70 (Z. Chen).

Reagents Supplied

The following reagents are supplied with this product:

Show all

NEB # Component Name Component # Stored at (°C) Amount Concentration

R0648S -20

Nb.BsrDI R0648SVIAL -20 1 x 0.1 ml 10,000 units/ml

rCutSmart™ Buffer B6004SVIAL -20 1 x 1.25 ml 10 X

R0648L -20

Nb.BsrDI R0648LVIAL -20 1 x 0.5 ml 10,000 units/ml

rCutSmart™ Buffer B6004SVIAL -20 1 x 1.25 ml 10 X

Product Categories:: Nicking Endonucleases Products,; Restriction Endonucleases: N-O

Properties & Usage
Unit Definition
One unit is defined as the amount of enzyme required to convert 1 µg of supercoiled pUC19 DNA to open circular form in 1 hour at 65°C in a total reaction volume of 50 µl.
Reaction Conditions

1X rCutSmart™ Buffer
Incubate at 65°C

1X rCutSmart™ Buffer
50 mM Potassium acetate
20 mM Tris-acetate
10 mM Magnesium acetate
100 µg/ml Recombinant Albumin
(pH 7.9 @ 25°C)

Activity in NEBuffers
NEBuffer™ r1.1: 25%
NEBuffer™ r2.1: 100%
NEBuffer™ r3.1: 100%
rCutSmart™ Buffer: 100%

Diluent Compatibility
- Diluent A
Storage Buffer

10 mM Tris-HCl
200 mM NaCl
1 mM DTT
0.1 mM EDTA
50% Glycerol
200 µg/ml BSA
pH 7.4 @ 25°C

Heat Inactivation
80°C for 20 min
Methylation Sensitivity

dam methylation: Not Sensitive
dcm methylation: Not Sensitive
CpG Methylation: Not Sensitive

Activity at Temperature
@37°C: 50%
Related Products
Companion Products
Materials Sold Separately
- rCutSmart™ Buffer
Product Notes
1. To see how this enzyme (along with other nicking enzymes) performs at different temperatures, please visit Effect of Various Temperatures of Different Nicking Endonucleases.
2. Not sensitive to CpG, dcm, or dam methylation.
References
1. Song, Q. et al. (2010). Anal. Chem. [Epub ahead of print].
2. Zhang, P. et al. (2010). Protein Expr. Purif. 69, 226-234. [Epub 2009 Sep 9].