Effect of Various Temperatures on Nicking Endonucleases

The following table indicates relative activity of nicking enzymes at different temperatures. Recommended incubation is noted (and normalized at 3-stars) and relative activity to that is indicated by the number of stars. When possible, NEB has chosen 37°C as the recommended temperature for its convenience, even if increased activity could be seen at an alternative temperature. For more information on nicking enzymes, please visit Nicking Endonucleases from NEB.

Temperature and Activity
Enzyme	Cutsite	Recommended temperature	20°C	37°C	40°C	50°C	60°C	70°C	80°C
Nt.AlwI		37°C	★	★★★	★★★★	★★★★	★★★★	-	-
Nb.BbvCI		37°C	★★	★★★	★★★	★★★	★★	★	-
Nt.BbvCI		37°C	★	★★★	★★★★	★★★★	★★	-	-
Nb.BsmI		65°C	-	-	★	★★	★★★	★★	-
Nt.BsmAI		37°C	★	★★★	★★★	★★★	-	-	-
Nt.BspQI		50°C	★	★★	★★	★★★	★	-	-
Nb.BsrDI		65°C	★	★★	★★★	★★★	★★★	★★★	★
Nb.BssSI		37°C	-	★★★	★★★	★★★★★	★★★★★	-	-
Nt.BstNBI		55°C	-	★	★	★★★	★★	-	-
WarmStart Nt.BstNBI		55°C	-	-	-	★★	★★★	-	-
Nb.BtsI		37°C	★★	★★★	★★★	★★★	★★★★	★★★★★	-
Nt.CviPII		37°C	★★	★★★	★★★	★★	★★	-	-