Having supplied restriction enzymes to the research community for over 40 years, NEB has earned the reputation of being the leader in enzyme technologies. Working continuously to be worth of that distinction, NEB strives to develop enzyme of the highest purity and unparalleled quality.
- This is a nicking endonuclease
- Generates DNA molecules that are “nicked”, rather than cleaved
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Double Digestion with NEBcloner
Nt.BstNBI is a site specific endonuclease that cleaves only one strand of DNA on a double-stranded DNA substrate. The nicking endonuclease catalyzes a single strand break 4 bases beyond the 3´ side of the recognition sequence.
- N.BstNB I catalyzes a single-stranded nick 3' of its recognition sequence.
Product SourceAn E. coli strain that carries the cloned Nt.BstNBI gene from Bacillus stereothermophilus 33M (Z. Chen).
The following reagents are supplied with this product:
Store at (°C) Concentration NEBuffer™ 3.1 -20 10 X
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