Having supplied restriction enzymes to the research community for over 40 years, NEB has earned the reputation of being the leader in enzyme technologies. Working continuously to be worthy of that distinction, NEB strives to develop enzymes of the highest purity and unparalleled quality.
- Time-Saver™ qualified for digestion in 5-15 minutes
- Used in Golden Gate Assembly
- sgRNA Template Construction for CRISPR/Cas Genome Editing
- Type IIS restriction enzymes recognize asymmetric DNA sequences and cleave outside of their recognition sequence
- High Fidelity (HF®) version available (NEB #R3539) supplied with CutSmart® Buffer
- Supplied with 1 vial of Gel Loading Dye, Purple (6X)
Restriction Enzymes in Golden Gate Assembly
Golden Gate Assembly Workflow
Reduce Star Activity with High-Fidelity Restriction Enzymes
Time-Saver Protocol for Restriction Enzyme Digests
NEB TV Ep. 15 – Applications of Restriction Enzymes
CutSmart Restriction Enzyme Buffer
Restriction Enzyme Digest Protocol: Cutting Close to DNA End
Restriction Enzyme Digestion Problem: DNA Smear on Agarose Gel
Why is My Restriction Enzyme Not Cutting DNA?
BbsI has a High Fidelity version BbsI-HF® (NEB #R3539) that can be stored at -20°C.
High Fidelity (HF) Restriction Enzymes have 100% activity in CutSmart Buffer; single-buffer simplicity means more straightforward and streamlined sample processing. HF enzymes also exhibit dramatically reduced star activity. HF enzymes are all Time-Saver qualified and can therefore cut substrate DNA in 5-15 minutes with the flexibility to digest overnight without degradation to DNA. Engineered with performance in mind, HF restriction enzymes are fully active under a broader range of conditions, minimizing off-target products, while offering flexibility in experimental design.
For details on NEB’s quality controls for restriction endonucleases, visit our Restriction Enzyme Quality page.
Product SourceAn E. coli strain that carries the cloned BbsI gene from Bacillus laterosporus (C. Story)
The following reagents are supplied with this product:
Store at (°C) Concentration NEBuffer™ 2.1 -20 10
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