EcoRV

Having supplied restriction enzymes to the research community for over 40 years, NEB has earned the reputation of being the leader in enzyme technologies. Working continuously to be worthy of that distinction, NEB strives to develop enzymes of the highest purity and unparalleled quality.
- Time-Saver™ qualified for digestion in 5-15 minutes
- High Fidelity (HF®) version available (NEB #R3195) supplied with CutSmart® Buffer
- Supplied with 1 vial of Gel Loading Dye, Purple (6X)
Featured Video
-
Product Information
EcoRV has a High Fidelity version EcoRV-HF® (NEB #R3195).
High Fidelity (HF) Restriction Enzymes have 100% activity in CutSmart Buffer; single-buffer simplicity means more straightforward and streamlined sample processing. HF enzymes also exhibit dramatically reduced star activity. HF enzymes are all Time-Saver qualified and can therefore cut substrate DNA in 5-15 minutes with the flexibility to digest overnight without degradation to DNA. Engineered with performance in mind, HF restriction enzymes are fully active under a broader range of conditions, minimizing off-target products, while offering flexibility in experimental design.Product Source
An E. coli strain that carries the EcoRV gene from plasmid J62 pLG74 (L.l. Glatman).Reagents Supplied
The following reagents are supplied with this product:
Store at (°C) Concentration Gel Loading Dye, Purple (6X) 25 6 X NEBuffer™ 3.1 -20 10 X - Product Categories:
- Restriction Endonucleases C G,
- time-saver-qualified-restriction-enzymes Products
-
Protocols, Manuals & Usage
-
Tools & Resources
-
FAQs & Troubleshooting
-
Quality, Safety & Legal
Featured Videos
-
Reduce Star Activity with High-Fidelity Restriction Enzymes
-
Time-Saver Protocol for Restriction Enzyme Digests
-
NEB TV Ep. 15 – Applications of Restriction Enzymes
-
CutSmart Restriction Enzyme Buffer
-
Restriction Enzyme Digest Protocol: Cutting Close to DNA End
-
Restriction Enzyme Digestion Problem: DNA Smear on Agarose Gel
-
Why is My Restriction Enzyme Not Cutting DNA?
-
Restriction Enzyme Digest Problem: Too Many DNA Bands
-
Double Digestion with NEBcloner
Other Products You May Be Interested In
Ineligible item added to cart
Based on your Freezer Program type, you are trying to add a product to your cart that is either not allowed or not allowed with the existing contents of your cart. Please review and update your order accordingly If you have any questions, please contact Customer Service at [email protected] or 1-800-632-5227 x 8.