EcoRV

We are excited to announce that all reaction buffers are now BSA-free. NEB began switching our BSA-containing reaction buffers in April 2021 to buffers containing Recombinant Albumin (rAlbumin) for restriction enzymes and some DNA modifying enzymes. Find more details at www.neb.com/BSA-free.

NEB restriction endonuclease that recognizes the sequence GAT_^ATC

Isoschizomers | Single Letter Code | Pronunciation:

Time-Saver™ qualified for digestion in 5-15 minutes
High Fidelity (HF^®) version available (NEB #R3195) supplied with rCutSmart^™Buffer
Supplied with 1 vial of Gel Loading Dye, Purple (6X)
Restriction Enzyme Cut Site: GAT/ATC

Catalog #	Concentration	Size	List Price	Your Price
R0195S	20,000 units/ml	4,000 units	$67.00	Sign In Sign In
R0195T	100,000 units/ml	4,000 units	$67.00	Sign In Sign In
R0195L	20,000 units/ml	20,000 units	$273.00	Sign In Sign In
R0195M	100,000 units/ml	20,000 units	$273.00	Sign In Sign In

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Product Information

EcoRV has a High Fidelity version EcoRV-HF^® (NEB #R3195).

High Fidelity (HF) Restriction Enzymes have 100% activity in rCutSmart Buffer; single-buffer simplicity means more straightforward and streamlined sample processing. HF enzymes also exhibit dramatically reduced star activity. HF enzymes are all Time-Saver qualified and can therefore cut substrate DNA in 5-15 minutes with the flexibility to digest overnight without degradation to DNA. Engineered with performance in mind, HF restriction enzymes are fully active under a broader range of conditions, minimizing off-target products, while offering flexibility in experimental design.

Product Source

An E. coli strain that carries the EcoRV gene from plasmid J62 pLG74 (L.l. Glatman).

This product is related to the following categories:: Restriction Endonucleases C G,; Time-Saver Qualified Restriction Enzymes Products

This product can be used in the following applications:: Fast Cloning: Accelerate your cloning workflows with reagents from NEB,; Restriction Enzyme Digestion

Reagents Supplied

The following reagents are supplied with this product:

NEB # Component Name Component # Stored at (°C) Amount Concentration

R0195S -20

EcoRV R0195SVIAL -20 1 x 0.2 ml 20,000 units/ml

NEBuffer™ r3.1 B6003SVIAL -20 1 x 1.25 ml 10 X

Gel Loading Dye, Purple (6X) B7024AVIAL 25 1 x 0.5 ml 6 X

R0195T -20

EcoRV R0195TVIAL -20 1 x 0.04 ml 100,000 units/ml

NEBuffer™ r3.1 B6003SVIAL -20 1 x 1.25 ml 10 X

Gel Loading Dye, Purple (6X) B7024AVIAL 25 1 x 0.5 ml 6 X

R0195L -20

EcoRV R0195LVIAL -20 1 x 1 ml 20,000 units/ml

NEBuffer™ r3.1 B6003SVIAL -20 1 x 1.25 ml 10 X

Gel Loading Dye, Purple (6X) B7024AVIAL 25 1 x 0.5 ml 6 X

R0195M -20

EcoRV R0195MVIAL -20 1 x 0.2 ml 100,000 units/ml

NEBuffer™ r3.1 B6003SVIAL -20 1 x 1.25 ml 10 X

Gel Loading Dye, Purple (6X) B7024AVIAL 25 1 x 0.5 ml 6 X

Properties & Usage
Unit Definition
One unit is defined as the amount of enzyme required to digest 1 µg of λ DNA in 1 hour at 37°C in a total reaction volume of 50 µl.
Reaction Conditions

1X NEBuffer™ r3.1
Incubate at 37°C

1X NEBuffer™ r3.1
100 mM NaCl
50 mM Tris-HCl
10 mM MgCl₂
100 µg/ml Recombinant Albumin
(pH 7.9 @ 25°C)

Activity in NEBuffers
NEBuffer™ r1.1: 10%
NEBuffer™ r2.1: 50%
NEBuffer™ r3.1: 100%
rCutSmart™ Buffer: 10%

Diluent Compatibility
- Diluent A
Storage Buffer

10 mM Tris-HCl
50 mM NaCl
1 mM DTT
0.1 mM EDTA
200 µg/ml BSA
50% Glycerol
pH 7.4 @ 25°C

Heat Inactivation
80°C for 20 minutes
Methylation Sensitivity

dam methylation: Not Sensitive
dcm methylation: Not Sensitive
CpG Methylation: Impaired by Some Combinations of Overlapping

Isoschizomers

Eco32I
EcoRV-HF
Related Products
Companion Products
Materials Sold Separately
- Gel Loading Dye, Purple (6X)
Product Notes
1. Cleaves to leave a ligatable blunt end in the tetracycline resistance gene of pBR322.
2. Impaired by some combinations of overlapping CpG methylation.