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SalI

We are excited to announce that all reaction buffers are now BSA-free. NEB began switching our BSA-containing reaction buffers in April 2021 to buffers containing Recombinant Albumin (rAlbumin) for restriction enzymes and some DNA modifying enzymes. Find more details at www.neb.com/BSA-free.

NEB restriction endonuclease that recognizes the sequence G^TCGA_C

Isoschizomers | Single Letter Code | Pronunciation:

Time-Saver™ qualified for digestion in 5-15 minutes
High Fidelity (HF^®) version available (NEB #R3138) supplied with rCutSmart^™ Buffer
Supplied with 1 vial of Gel Loading Dye, Purple (6X)
Restriction Enzyme Cut Site: G/TCGAC

Catalog #	Concentration	Size	List Price	Your Price
R0138S	20,000 units/ml	2,000 units	$69.00	Sign In Sign In
R0138T	100,000 units/ml	2,000 units	$69.00	Sign In Sign In
R0138L	20,000 units/ml	10,000 units	$283.00	Sign In Sign In
R0138M	100,000 units/ml	10,000 units	$283.00	Sign In Sign In

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Product Information

SalI has a High Fidelity version SalI-HF^® (NEB #R3138).

High Fidelity (HF) Restriction Enzymes have 100% activity in rCutSmart Buffer; single-buffer simplicity means more straightforward and streamlined sample processing. HF enzymes also exhibit dramatically reduced star activity. HF enzymes are all Time-Saver™ qualified and can therefore cut substrate DNA in 5-15 minutes with the flexibility to digest overnight without degradation to DNA. Engineered with performance in mind, HF restriction enzymes are fully active under a broader range of conditions, minimizing off-target products, while offering flexibility in experimental design.

Product Source

An E. coli strain that carries the SalI gene from Streptomyces albus G (ATCC 49789).

This product is related to the following categories:: Restriction Endonucleases S,; Time-Saver Qualified Restriction Enzymes Products

This product can be used in the following applications:: Fast Cloning: Accelerate your cloning workflows with reagents from NEB,; Restriction Enzyme Digestion

Reagents Supplied

The following reagents are supplied with this product:

NEB # Component Name Component # Stored at (°C) Amount Concentration

R0138S -20

SalI R0138SVIAL -20 1 x 0.1 ml 20,000 units/ml

NEBuffer™ r3.1 B6003SVIAL -20 1 x 1.25 ml 10 X

Gel Loading Dye, Purple (6X) B7024AVIAL 25 1 x 0.5 ml 6 X

R0138T -20

SalI R0138TVIAL -20 1 x 0.02 ml 100,000 units/ml

NEBuffer™ r3.1 B6003SVIAL -20 1 x 1.25 ml 10 X

Gel Loading Dye, Purple (6X) B7024AVIAL 25 1 x 0.5 ml 6 X

R0138L -20

SalI R0138LVIAL -20 1 x 0.5 ml 20,000 units/ml

NEBuffer™ r3.1 B6003SVIAL -20 1 x 1.25 ml 10 X

Gel Loading Dye, Purple (6X) B7024AVIAL 25 1 x 0.5 ml 6 X

R0138M -20

SalI R0138MVIAL -20 1 x 0.1 ml 100,000 units/ml

NEBuffer™ r3.1 B6003SVIAL -20 1 x 1.25 ml 10 X

Gel Loading Dye, Purple (6X) B7024AVIAL 25 1 x 0.5 ml 6 X

Properties & Usage
Unit Definition
One unit is defined as the amount of enzyme required to digest 1 µg of λ DNA (HindIII digest) in 1 hour at 37°C in a total reaction volume of 50 µl.
Reaction Conditions

1X NEBuffer™ r3.1
Incubate at 37°C

1X NEBuffer™ r3.1
100 mM NaCl
50 mM Tris-HCl
10 mM MgCl₂
100 µg/ml Recombinant Albumin
(pH 7.9 @ 25°C)

Activity in NEBuffers
NEBuffer™ r1.1: <10%
NEBuffer™ r2.1: <10%
NEBuffer™ r3.1: 100%
rCutSmart™ Buffer: <10%

Diluent Compatibility
- Diluent A
Storage Buffer

10 mM Tris-HCl
50 mM KCl
1 mM DTT
0.1 mM EDTA
300 µg/ml BSA
50% Glycerol
pH 7.5 @ 25°C

Heat Inactivation
65°C for 20 minutes
Methylation Sensitivity

dam methylation: Not Sensitive
dcm methylation: Not Sensitive
CpG Methylation: Blocked

Isoschizomers

SalI-HF
Related Products
Companion Products
Product Notes
1. This enzyme has shown to have lower activity on some supercoiled plasmids, with more than 1 unit required to digest 1 μg plasmid DNA. For complete digestion of 1 μg of plasmid DNA please follow our recommended digestion protocol.
2. Cleavage of mammalian genomic DNA is blocked by CpGmethylation.