AatII

Reagents Supplied

The following reagents are supplied with this product:

NEB #	Component Name	Component #	Stored at (°C)	Amount	Concentration
R0117S     -20       AatII R0117SVIAL -20 1 x 0.025 ml 20,000 units/ml   rCutSmart™ Buffer B6004SVIAL -20 1 x 1.25 ml 10 X   Gel Loading Dye, Purple (6X) B7024AVIAL 25 1 x 0.5 ml 6 X
R0117L     -20       AatII R0117LVIAL -20 1 x 0.125 ml 20,000 units/ml   rCutSmart™ Buffer B6004SVIAL -20 1 x 1.25 ml 10 X   Gel Loading Dye, Purple (6X) B7024AVIAL 25 1 x 0.5 ml 6 X

Companion Products

• Monarch® Plasmid Miniprep Kit 
• Monarch® DNA Gel Extraction Kit
• Monarch® PCR & DNA Cleanup Kit (5 μg)

Materials Sold Separately

• rCutSmart™ Buffer
• Gel Loading Dye, Purple (6X)

1. Activity decreases if reaction buffer pH is not between 7.5 and 8.0 @25°C.
2. May exhibit star activity in NEBuffer r2.1.
3. Blocked by CpG methylation.

1. Optimizing Restriction Endonuclease Reactions
2. Restriction Digest Protocol
3. Double Digest Protocol with Standard Restriction Enzymes

• Activity at 37°C for Restriction Enzymes with Alternate Incubation Temperatures
• Activity of Restriction Enzymes in PCR Buffers
• Cleavage Close to the End of DNA Fragments
• Digestion of Agarose-Embedded DNA: Info for Specific Enzymes
• Double Digests
• Effects of CpG Methylation on Restriction Enzyme Cleavage
• Heat Inactivation
• Optimizing Restriction Endonuclease Reactions
• Restriction Endonucleases - Survival in a Reaction
• Restriction Enzyme Diluent Buffer Compatibility
• Restriction Enzyme Tips
• Single Letter Codes
• Star Activity

• Alphabetized List of Recognition Sequences
• Cleavage of Supercoiled DNA
• Compatible Cohesive Ends and Generation of New Restriction Sites
• Dam-Dcm and CpG Methylation
• Frequencies of Restriction Sites
• Isoelectric Points (pI) for Restriction Enzymes
• Isoschizomers
• NEB Diluent and Buffer Table
• Time-Saver™ Qualified Enzymes

• DNA Sequences and Maps Tool

• Enzyme Finder
• NEBcutter™ v3.0
• NEBioCalculator®
• REBASE®

1. Are there any factors that inhibit cleavage by AatII?
2. Is AatII active at 25°C?
3. Do I have to set-up digests with Time-Saver™ qualified enzymes for 5-15 minutes? Can I digest longer?
4. I tested your restriction enzyme on the substrate DNA recommended by NEB, and it appears to be active, however it does not digest my DNA. What could be the reason?
5. Why is my Restriction Enzyme not cutting DNA?
6. Are Protease Inhibitors acceptable for use with the Protein Deglycosylation Mix reaction?
7. Why do I see a DNA smear on an agarose gel after a restriction digest?
8. Why do I see additional DNA bands on my gel after a restriction digest?
9. How many nucleotides do I have to add adjacent to the RE recognition site in order to get efficient cutting?
10. Why is my Restriction Enzyme not cutting DNA?
11. Which NEB restriction enzymes are supplied with Gel Loading Dye, Purple (6X)?
12. Is this enzyme sensitive to dam, dcm or mammalian CpG methylation?
13. Can you tell me more about the switch from BSA to Recombinant Albumin (rAlbumin) in NEBuffers?
14. Can Gel Loading Dye, Purple 6X (B7024) be stored in cold temperatures?

• Nwankwo, D.O., Maunus, R.E., and Xu, S.Y. (1997) Cloning and expression of AatII restriction-modification system in Escherichia coli. Gene; 185, 105-109. PubMedID: 9034320

Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.

Specifications & Change Notifications

The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]

• R0117S_L_v1

The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]

• R0117S_L_v1_0671301
• R0117S_L_v1_0671306
• R0117S_L_v1_0671310
• R0117S_L_v1_0671403
• R0117S_L_v1_0671411
• R0117S_L_v1_0711503
• R0117S_L_v1_0711508
• R0117S_L_v1_0711512
• R0117S_L_v1_0711608
• R0117S_L_v1_0711702
• R0117S_L_v1_0711708
• R0117S_L_v1_0711803
• R0117S_v1_10019727
• R0117L_v1_10027304
• R0117S_v1_10032851
• R0117L_v1_10035112
• R0117S_v1_10044764
• R0117L_v1_10044762
• R0117S_v1_10055036
• R0117L_v1_10062617
• R0117S_v1_10062619
• R0117S_v1_10069257
• R0117L_v1_10074542
• R0117S_v1_10081830
• R0117L_v1_10081833
• R0117L_v1_10089950
• R0117S_v1_10087610
• R0117L_v1_10092653
• R0117S_v1_10096370
• R0117L_v1_10100444
• R0117S_v1_10124028
• R0117L_v1_10129292
• R0117L_v1_10142689
• R0117S_v1_10142681
• R0117S_v1_10142691
• R0117L_v1_10155985
• R0117S_v1_10163963
• R0117S_v1_10179301

The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.

• AatII
• rCutSmart™ Buffer
• Gel Loading Dye, Purple (6X)

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This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

New England Biolabs (NEB) is committed to practicing ethical science – we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner. Learn more.