Nucleic Acid Purification
Workflows for detecting, analyzing, amplifying or manipulating DNA and RNA often require extraction and purification from a biological sample and/or enzymatic reactions. Monarch nucleic acid purification kits provide fast and reliable purification of high-quality DNA and RNA from a variety of sources using best-in-class silica column technology, and using a novel glass bead-based approach for HMW DNA extraction. DNA and RNA purified with Monarch Kits is highly-pure and suitable for use in a wide variety of downstream applications including sequencing, cloning, PCR and other enzymatic manipulations. Monarch kits are all designed with sustainability in mind; they use less plastic and are packaged in responsibly-sourced, recyclable material. For convenience, all Monarch kit components are also available separately.
Featuring: The Monarch Mag Viral DNA/RNA Extraction Kit provides a rapid and reliable magnetic bead-based process for extracting viral nucleic acids from saliva and swab samples, and is compatible with wastewater.
Learn more about Monarch Kits, and request a free sample, at NEBMonarch.com.
See why Monarch Kits are an excellent choice for:
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- Can I use water to elute the DNA when using the Monarch Kits?
- What is the maximum binding capacity of the Monarch DNA Cleanup Column provided in the Monarch DNA Gel Extraction Kit?
- What is the maximum binding capacity of the Monarch DNA Cleanup Column provided with the Monarch PCR & DNA Cleanup Kit (5 μg)?
- Are plasmids recovered using the Monarch Plasmid Miniprep Kit endotoxin free?
- Can I use the Monarch DNA & PCR Cleanup Kit to purify oligonucleotides and other short DNA fragments?
- I have used 5 ml of cell culture for plasmid isolation with the Monarch Plasmid Miniprep kit and I am obtaining low amounts of plasmid and/or contaminating gDNA. Why is this, and what are your suggestions to improve yield and purity?
- How important is it to pre-heat the elution buffer to 60°C? Would 56°C be OK?
- The enzymes in the kit were not stored at -20°C right away. Will they still work?
- What is the composition of each buffer provided with the Monarch® Spin gDNA Extraction Kit?
- What is the maximum binding capacity of the Monarch® Spin gDNA Extraction Kit columns?
- What is the minimum elution volume that can be used with the Monarch® Spin gDNA Extraction Kit columns?
- Can I get better recovery with the Monarch® Spin gDNA Extraction Kit if I do a second elution with my eluent from the first elution?
- What size gDNA can be purified with the Monarch® Spin gDNA Extraction Kit?
- Can I omit the low-speed binding centrifugation step to save time?
- Can I omit the RNase A digestion step from the protocol?
- Can I use a different Proteinase K with the Monarch® Genomic DNA Purification Kit?
- Can I use a different RNase A with the Monarch® Spin gDNA Extraction Kit?
- Can I use Monarch® RNase A for applications/workflows other than gDNA purification?
- Can I use the Monarch® Spin gDNA Extraction Kit for gDNA cleanup?
- Can I use the Monarch® Spin gDNA Extraction Kit to clean up gDNA that was extracted with phenol/chloroform?
- Does it matter which anticoagulant is used in blood samples?
- How can I assess gDNA integrity and purity?
- How should I store purified gDNA samples?
- Is Monarch-purified gDNA compatible with Next Generation Sequencing (NGS) platforms like PacBio and nanopore?
- Is Monarch-purified gDNA suitable for long-range PCR?
- What is the minimal input amount that can be used with the Monarch® Spin gDNA Extraction Kit?
- When working with other commercial silica column-based kits, I occasionally see a white precipitate in the eluate. What is this?
- Monarch® Plasmid DNA Miniprep Kit Protocol (NEB #T1010)
- Monarch® DNA Gel Extraction Kit Protocol (NEB #T1020)
- Protocol for DNA Cleanup and Concentration Using the Monarch® PCR & DNA Cleanup Kit (5 μg) (NEB #T1030)
- Oligonucleotide Cleanup Using Monarch® PCR & DNA Cleanup Kit (5 μg) Protocol (NEB #T1030)
- Total RNA Purification from Mammalian Whole Blood Samples using the Monarch Total RNA Miniprep Kit (NEB #T2010)
- Total RNA Purification from Cultured Mammalian Cells using the Monarch Total RNA Miniprep Kit (NEB #T2010)
- Total RNA Purification from Tissues and Leukocytes using the Monarch Total RNA Miniprep Kit (NEB #T2010)
- Total RNA Purification from Tough-to-Lyse Samples (bacteria, yeast, plant, etc.) using the Monarch Total RNA Miniprep Kit (NEB #T2010)
- Quick Protocol for Total RNA Extraction (NEB #T2010)
- Monarch® Spin RNA Cleanup Kit Protocol
- Protocol for Extraction and Purification of Genomic DNA from Cells (NEB #T3010)
- Protocol for Extraction and Purification of Genomic DNA from Blood (NEB #T3010)
- Protocol for Extraction and Purification of Genomic DNA from Tissues (NEB #T3010)
- Genomic DNA Extraction from Buccal Swabs (NEB #T3010)
- Genomic DNA Extraction from Gram-negative Bacteria (NEB #T3010)
- Genomic DNA Extraction from Gram-positive Bacteria and Archaea (NEB #T3010)
- Genomic DNA Extraction from Saliva (NEB #T3010)
- Genomic DNA Extraction from Yeast (NEB #T3010)
- Genomic DNA Cleanup Protocol
- Quick Protocol for Extraction and Purification of Genomic DNA
- Protocol for High Molecular Weight DNA (HMW DNA) Extraction from Blood (NEB #T3050)
- Protocol for High Molecular Weight DNA (HMW DNA) Extraction from Cells (NEB #T3050)
- Protocol for High Molecular Weight DNA (HMW DNA) Extraction from Bacteria (NEB #T3060)
- Protocol for High Molecular Weight DNA (HMW DNA) Extraction from Tissue (NEB #T3060)
- Protocol for Isolation of Genomic DNA (gDNA) from up to 500 µl mammalian blood using the Monarch Spin gDNA Extraction Kit (NEB #T3010) and RBC Lysis Buffer (NEB #T3051)
- Protocol for High Molecular Weight DNA (HMW DNA) Extraction from Yeast (NEB #T3060)
- Using Exonuclease V (RecBCD) to Eliminate Residual Genomic DNA When Purifying Low Copy Plasmids with the Monarch® Plasmid Miniprep Kit
- A Practical Guide to Analyzing Nucleic Acid Concentration and Purity with Microvolume Spectrophotometers
- Purification of synthetic SARS-CoV-2 viral RNA from biological samples using the Monarch® Total RNA Miniprep Kit and the Monarch RNA Cleanup Kit
- A faster workflow for the assessment of genomic loci in mice using a novel HMW DNA extraction technology upstream of Cas9 targeted sequencing
- Monarch HMW DNA Extraction Kit improves sample preparation for Oxford Nanopore Technologies sequencing of malaria parasites
- Facilitating Purification and Detection of Viral Nucleic Acids from Milk
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- Monarch Nucleic Acid Purification Brochure
- Troubleshooting Guide for DNA Cleanup and Plasmid Purification
- Troubleshooting Guide for Genomic DNA Extraction & Purification (NEB #T3010)
- Troubleshooting Guide for Monarch HMW DNA Extraction Kits (NEB #T3050 and #T3060)
- Troubleshooting Guide for RNA Cleanup
- Troubleshooting Guide for Total RNA Extraction & Purification
- Back to basics: Important things to keep in mind when purifying plasmids and DNA fragments
- Choosing Input Amounts for the Monarch HMW DNA Extraction Kits
- Choosing Input Amounts for the Monarch Spin gDNA Extraction Kit
- Choosing an Agitation Speed During Lysis with the Monarch HMW DNA Extraction Kits
- Considerations and Performance Data for Nanopore Sequencing of High Molecular Weight DNA (HMW DNA)
- Considerations for Loading Pulsed-Field Gels (PFG) with High Molecular Weight DNA
- Dos and Don'ts of Plasmid Minipreps
- Factors Affecting DNA Quality when Purifying gDNA from Blood and Tissues with the Monarch® Spin gDNA Extraction Kit
- General Guidelines for Successful RNA Purification Using the Monarch Total RNA Miniprep Kit
- Guidance on Choosing Sample Input Amounts when Using the Monarch Total RNA Miniprep Kit
- Guidelines for Handling Tissue Samples when using the Monarch Spin gDNA Extraction Kit
- Guidelines for RNA Quantitation
- Guidelines for Working with RNA During RNA Cleanup
- Homogenization of High Molecular Weight DNA (HMW DNA) Samples After Elution
- Important Considerations for Starting Materials When Using the Monarch HMW DNA Extraction Kit for Cells & Blood (NEB #T3050)
- Important Considerations for Starting Materials When Using the Monarch HMW DNA Extraction Kit for Tissues (NEB #T3060)
- Measuring, Analyzing & Storing High Molecular Weight DNA (HMW DNA) Samples
- Six Tips for a Perfect Gel Extraction
- Tips for the Monarch PCR & DNA Cleanup Kit
- Usage Guidelines for the Monarch Plasmid Miniprep Kit (#T1010) When Working with Low Copy Plasmids
- A Novel Method for High Molecular Weight (HMW) DNA Extraction
Feature Articles
Brochures
Troubleshooting Guides
Usage Guidelines
Posters
Product Manual for the Monarch HMW DNA Extraction Kit for Cells & Blood
Product Manual for the Monarch HMW DNA Extraction Kit for Tissue
Product Manual for the Monarch Plasmid Miniprep Kit
Product Manual for the Monarch PCR & DNA Cleanup Kit (5 µg)
Product Manual for the Monarch DNA Gel Extraction Kit
Product Manual for the Monarch Total RNA Miniprep Kit
Products and content are covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB). The use of trademark symbols does not necessarily indicate that the name is trademarked in the country where it is being read; it indicates where the content was originally developed. The use of this product may require the buyer to obtain additional third-party intellectual property rights for certain applications. For more information, please email busdev@neb.com.
This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.
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Overview of High Molecular Weight DNA Extraction using the Monarch® HMW DNA Extraction Kit for Cells & Blood
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Fast and Efficient Extraction of High Molecular Weight DNA
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Monarch® Plasmid Miniprep Kit protocol
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Monarch® DNA Gel Extraction Kit protocol
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Monarch® PCR & DNA Cleanup Kit Protocol
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Tips for using the Monarch® Plasmid Miniprep Kit
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Tips for using the Monarch® DNA Gel Extraction Kit
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Tips for using the Monarch® PCR & DNA Cleanup Kit
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Monarch® Total RNA Miniprep Protocol
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Tips for Successful RNA Purification using Monarch® Total RNA Miniprep Kit
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How to recycle your Monarch® Kit components
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Tips for Handling and Storing Tissue Samples Prior to DNA Extraction
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Post-lysis Tips for Optimal Results using the Monarch® Spin gDNA Extraction Kit
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Tips for Efficient Lysis of Tissue Samples Using the Monarch® Spin gDNA Extraction Kit
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Tips for Successful Genomic DNA Extraction from Blood Samples
