FAQ: Can I use the Monarch® Genomic DNA Purification Kit for gDNA cleanup?

Yes, the Monarch Genomic DNA Purification Kit can be used for cleaning up gDNA from enzymatic reactions or previous purification protocols by following the protocol for Genomic DNA Cleanup. Cleanup can be performed either directly on the gDNA or with a prior enzyme inactivation step using Proteinase K. 

Recovery of approximately 80% gDNA may be expected with 1 elution step when the input amount is >100 ng. However, if the input DNA is longer than 80 kb, which may be the case with gDNA that was purified following, for example, phenol-chloroform or salting-out protocols, the elution efficiency may be lower because gDNA >80 kb is not eluted efficiently from the silica matrix.