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DNA Cleanup

The Monarch PCR & DNA Cleanup Kit reliably purifies up to 5 µg of concentrated, high quality DNA from PCR and other enzymatic reactions. It utilizes a bind/wash/elute workflow with minimal incubation and spin times. The columns provided with each kit ensure zero buffer retention and no carryover of contaminants, enabling elution in volumes as low as 6 µl. Monarch Buffers have been optimized, and do not require pH monitoring. Eluted DNA is ready for use in restriction digests, DNA sequencing, PCR, ligation, and other enzymatic manipulations.

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Our unique column design offers several improvements over other commercially available products, including the elimination of buffer retention and elution in as little as 6 µl

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FAQs for DNA Cleanup
Protocols for DNA Cleanup
Application Notes for DNA Cleanup
Product Manual
Advantages of the Monarch PCR & DNA Cleanup Kit
  • Elute in as little as 6 μl
  • Prevent buffer retention and salt carryover with optimized column design
  • Save time with fast, user-friendly protocols
  • No need to monitor pH
  • Buffers and columns available separately
  • Significantly less plastic used when compared with other kits
  • Responsibly-sourced and recyclable packaging
Specifications of the Monarch PCR & DNA Cleanup Kit
  • Binding Capacity: up to 5 μg
  • DNA Size Range:
    ~50 bp to 25 kb
    DNA ≥  15 bp to 25 kb (dsDNA) and DNA ≥ 18 nt to 10 kb (ssDNA) can also be purified using the Oligonucleotide Cleanup Protocol.
  • Typical Recovery:
    DNA (50 bp to 10 kb): 70–90%
    DNA (11–25 kb): 50–70%
    ssDNA ≥  18 nt and dsDNA ≥ 15 bp: 70-85%
  • Elution Volume: ≥ 6 μl
  • Purity: A260/280 ≥ 1.8
  • Protocol Time: 5 min of spin and incubation time
  • Compatible Downstream Applications: ligation, restriction digestion, labeling and other enzymatic manipulations, library construction and DNA sequencing
Legal Information

Products and content are covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB). The use of trademark symbols does not necessarily indicate that the name is trademarked in the country where it is being read; it indicates where the content was originally developed. The use of this product may require the buyer to obtain additional third-party intellectual property rights for certain applications. For more information, please email

This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

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