DNA Cleanup
Improved performance by design
Our unique column design offers several improvements over other commercially available products, including the elimination of buffer retention and elution in as little as 6 µl.
Choose Type:
- What factors affect the (A260/230) ratio when using the Monarch® Spin PCR & DNA Cleanup Kit (NEB #T1130)?
- Can I purchase additional Monarch® columns and collection tubes for the MonarchSpin DNA Gel Extraction Kit (NEB #T1120) and the Monarch Spin PCR & DNA Cleanup Kit (NEB #T1130) separately?
- Can the Monarch® Spin PCR & DNA Cleanup Kit (5 μg) (NEB #T1130) be used to purify RNA?
- Can I use the Monarch® Spin PCR & DNA Cleanup Kit (NEB #T1130) to purify oligonucleotides and other DNA fragments?
- Are the components of the previous version of Monarch® PCR & DNA Cleanup Kit (NEB #T1030) compatible with the new version? Can I use #T1030 buffers on new Monarch Spin Columns S1A (NEB #T2037) or vice versa?
- Can the Monarch PCR & DNA Cleanup Kit (5 μg) be used to purify RNA?
- Buffer Preparation Guidance for Monarch® Spin PCR & DNA Cleanup Kit (5 µl) (NEB #T1130)
- Oligonucleotide Cleanup Protocol for Monarch® Spin PCR & DNA Cleanup Kit (NEB #T1130)
- Standard Cleanup Protocol using the Monarch® Spin PCR & DNA Cleanup Kit and a Vacuum Manifold (NEB #T1130)
- Standard Cleanup Protocol using the Monarch® Spin PCR & DNA Cleanup Kit and Centrifugation (NEB #T1130)
- Monarch Nucleic Acid Purification Brochure
- Troubleshooting Guide for DNA Cleanup and Plasmid Purification
- Back to basics: Important things to keep in mind when purifying plasmids and DNA fragments
- Tips for the Monarch PCR & DNA Cleanup Kit
Brochures
Troubleshooting Guides
Usage Guidelines
- Elute in as low as 5 µl for highly concentrated DNA
- Prevent buffer retention and salt carry over with unique, optimized column design
- Reduce hands-on time with faster protocols and less spin timeNo need to monitor pH
- Protocol modification for oligonucleotide cleanup is provided, allowing purification of ssDNA, oligonucleotide and other small DNA fragments
- Significantly less plastic by design in columns and kit compared to leading suppliers
| Properties | |
|---|---|
| Purification format | Spin-column |
| DNA sample |
DNA from PCR and other enzymatic reactions (e.g., restriction digests, kinase reactions, ligations) |
| ssDNA or dsDNA oligonucleotides from enzymatic reactions can also be purified using the Oligonucleotide Cleanup Protocol | |
| Typical recovery |
70-90% |
| DNA Purity | A260/280 > 1.8 and A260/230 > 1.8 |
| Binding Capacity | Up to 5 μg |
| Elution Volume | 5-20 μl |
| DNA Size Range |
Standard protocol: 50 bp – 25 kb |
| Protocol Time | 5 minutes of spin and incubation time |
| Compatible Downstream Applications | Ligation, restriction digestion, labeling and other enzymatic manipulations, library construction and DNA sequencing |
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This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.
