FAQ: What is the maximum binding capacity of the Monarch DNA Cleanup Column provided with the Monarch PCR & DNA Cleanup Kit (5 μg)?

The matrix supplied in each column is sufficient to bind 5 micrograms of purified DNA under testing conditions. Purifying DNA from a PCR or other enzymatic reaction introduces components such as detergents, dyes, protein, and nucleotides; all of which can compete for binding to the membrane and affect recovery of the DNA of interest.