DNA Gel Extraction
Improved performance by design
Our unique column design offers several improvements over other commercially available products, including the elimination of buffer retention and elution in as little as 6 µl.
Choose Type:
- What type of agarose gels are compatible with the Monarch® Spin DNA Gel Extraction Kit (NEB #T1120)?
- Are the columns in the Monarch® Spin PCR & DNA Cleanup Kit (5 μg) (NEB #T1130) the same as those in the Monarch Spin DNA Gel Extraction Kit (NEB #T1120)?
- Can I purchase additional Monarch® columns and collection tubes for the MonarchSpin DNA Gel Extraction Kit (NEB #T1120) and the Monarch Spin PCR & DNA Cleanup Kit (NEB #T1130) separately?
- Are the components of the previous version of Monarch® DNA Gel Extraction Kit (NEB #T1020) compatible with the new version? Can I use #T1020 buffers on new Monarch Spin Columns S1A or vice versa?
- Can the Monarch PCR & DNA Cleanup Kit (5 μg) be used to purify RNA?
- Buffer Preparation Guidance for Monarch® Spin DNA Gel Extraction Kit (NEB #T1120)
- Standard Gel Extraction Protocol using the Monarch® Spin DNA Gel Extraction Kit and a Vacuum Manifold (NEB #T1120)
- Standard Gel Extraction Protocol using the Monarch® Spin DNA Gel Extraction Kit and Centrifugation (NEB #T1120)
- Monarch Nucleic Acid Purification Brochure
- Troubleshooting Guide for DNA Cleanup and Plasmid Purification
- Back to basics: Important things to keep in mind when purifying plasmids and DNA fragments
- Six Tips for a Perfect Gel Extraction
Brochures
Troubleshooting Guides
Usage Guidelines
- High yields (up to 5 μg) and high purity in the extraction and purification of DNA from agarose gels, with the capability to remove contaminants and residual salts.
- Elute in as little as 5 μl for elution, allowing for highly concentrated DNA.
- A short protocol of only 10 minutes of spin and incubation time is needed to complete the workflow.
- Spin column features a unique design that enables elution in low volumes and minimizes buffer retention and contaminant carryover.
- No need to adjust pH or add isopropanol.
- Purified DNA is ready for downstream molecular applications, such as restriction digests, DNA sequencing, ligation, amplification, and other enzymatic reactions.
| Properties | |
|---|---|
| Purification format | Spin column for nucleic acid purification |
| Compatible methods | Centrifugation or vacuum manifold |
| DNA sample |
dsDNA from agarose gels |
| Typical recovery |
70-90% for < 10 kb and 50-70% for ≥ 10 kb |
| DNA Purity | A260/280 > 1.8 and A260/230 > 1.8 |
| Binding Capacity | Up to 5 μg |
| Elution Volume | ≥ 5 μl |
| DNA Size Range |
Standard protocol: 100 bp – 25 kb |
| Protocol Time | 10 - 15 minutes of spin and incubation time |
| Compatible Downstream Applications | Ligation, restriction digestion, labeling and other enzymatic manipulations, library construction and DNA sequencing |
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This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.
