Markers and Ladders
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  • DNA Markers & Ladders

    NEB offers a variety of DNA Ladders with sizes ranging from 25 bp to 40 kb for use in agarose gel electrophoresis. NEB also offers a wide range of double-stranded DNA molecular weight markers for conventional agarose gel electrophoresis. These standards have a size range of approximately 10 bp to 23,000 base pairs.

    The typical pattern generated by each of the conventional markers is to the right. The number of fragments generated by each marker, as well as the specific fragment sizes for each of the conventional markers can be found on the product page.

    Our growing line of DNA Ladders now includes the Mass and Fast DNA Ladders. The Mass DNA Ladder is ideal for size determination and mass quantification in the range of 0.5 – 10 kb. The Fast DNA Ladder is designed for use with fast electrophoresis systems.

    Convenient Formats

    The TriDye™ DNA Ladders are pre-mixed, ready-to-load molecular weight markers containing 3 dyes which serve as visual aids to monitor the progress of migration during agarose gel electrophoresis.

    The Quick-Load® DNA Ladders are pre-mixed, ready-to-load molecular weight markers containing bromophenol blue as a tracking dye.

    For more information on these ready-to-use formats, see the tab below.

    TriDye™ is a trademark of New England Biolabs.
    Quick-Load® is a registered trademark of New England Biolabs.

    Featured Products

      Other Tools & Resources

      Brochures

    • Protein Expression & Purification Brochure

      The Protein Expression and Purification brochure provides information on the advanced tools for protein expression and purification offered by NEB.

    • PCR Brochure

      The PCR brochure provides product information on the wide range of DNA polymerases available from NEB, including tools for selection and troubleshooting tips.

    Advantages

    • Suitable for analysis of a wide range of DNAs
    • Uniform band intensities and convenient band spacing
    • Easy-to-identify reference bands

    TriDye and Quick-Load Formats

    • TriDye™ Ladders: 1 kb, 100 bp and 2-Log DNA Ladders are available in ready-to-load format containing xylene cyanol FF, bromophenol blue and orange G.
    • Quick-Load® Ladders: 1 kb, 1 kb extend, 50 bp, 100 bp, 2-Log, Low Molecular Weight DNA Ladders and PCR marker are available in ready-to-load format containing bromophenol blue.

    Comparison of NEB to Other Commercially Available DNA Ladders

    Comparison of the NEB 1 kb (left) and 100 bp (right) DNA Ladders to other commercially available DNA Ladders. Note: The 500 bp DNA Ladder from Roche is used in the 1 kb DNA Ladder comparison.

    DNA Markers & Ladders Selection Chart