DNA Markers & Ladders

NEB offers a variety of DNA Ladders with sizes ranging from 25 bp to 48.5 kb for use in agarose gel electrophoresis. NEB also offers a wide range of double-stranded DNA molecular weight markers for conventional agarose gel electrophoresis. These standards have a size range of approximately 10 bp to 23,000 base pairs. Ladders are also available for pulse-field gel electrophoresis.

NEB’s DNA ladders are offered in 4 convenient formats:

Conventional (with no loading dye)
  • Available in a wide variety of size ranges
  • Most economical of the 4 formats
Quick-Load® Purple format
  • Ready-to load
  • Contains a non-fluorescing purple dye which does not cast a UV shadow
  • Optimized to create sharp, tight bands
  • Available for the Low Molecular Weight50 bp100 bp1 kb, and 1 kb Plus DNA Ladders.
  • Value pricing
Quick-Load format
  • Ready-to-load
  • Contains bromophenol blue dye
Tri-Dye® format

request a sample 

The typical pattern generated by each of our markers and ladders is shown below. Fragment sizes are indicated alongside of the image. Relative mass values are also shown, when appropriate.





UV Shadow Comparison


The new Gel Loading Dye, Purple (6X) (Lane 1) included in the Quick-Load Purple 1 kb DNA Ladder does not cast a UV shadow over the underlying bands, unlike the Gel Loading Dye, Blue (6X) (Lane 2).

FAQs for DNA Markers & Ladders

Protocols for DNA Markers & Ladders

    Other Tools & Resources

    Brochures

  • PCR Brochure

    The PCR brochure provides product information on the wide range of DNA polymerases available from NEB, including tools for selection and troubleshooting tips.

  • Protein Expression & Purification Brochure

    The Protein Expression and Purification brochure provides information on the advanced tools for protein expression and purification offered by NEB.

Advantages

  • DNA Ladders are supplied with 1 vial of Gel Loading Dye
  • Suitable for analysis of a wide range of DNAs
  • Uniform band intensities and convenient band spacing
  • Easy-to-identify reference bands
  • DNA Ladders can be used for sample quantitation

TriDye and Quick-Load Formats

  • TriDye™ Ladders: 1 kb, 100 bp and 1 kb Plus DNA Ladders are available in ready-to-load format containing xylene cyanol FF, bromophenol blue and orange G.
  • Quick-Load® Ladders: 1 kb, 1 kb extend, 50 bp, 100 bp, 1 kb Plus, Low Molecular Weight DNA Ladders and PCR marker are available in ready-to-load format containing either non-fluorescing purple dye or bromophenol blue as a tracking dye.

Comparison of NEB to Other Commercially Available DNA Ladders

Comparison of the NEB 1 kb (left) and 100 bp (right) DNA Ladders to other commercially available DNA Ladders. Note: The 500 bp DNA Ladder from Roche is used in the 1 kb DNA Ladder comparison.

DNA Markers & Ladders Selection Chart

Legal Information

This product is covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB).

While NEB develops and validates its products for various applications, the use of this product may require the buyer to obtain additional third party intellectual property rights for certain applications.

For more information about commercial rights, please contact NEB's Global Business Development team at gbd@neb.com.

This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

  1. HowToPrepareandLoadAStandardDNALadder_1920

    How to Prepare and Load a Standard DNA Ladder

    In this video, you will learn how to prepare a standard DNA ladder and load it onto an agarose gel.

  2. HowToUseandLoadAPulseFieldGelMarker_1920

    How to Prepare and Load a Pulse Field Gel Marker

    In this video, you will learn how to prepare a pulse field gel marker and load it onto an agarose gel.

  3. WhatIsPFGE_720

    What is Pulse Field Gel Electrophoresis?

    Learn the basics of how large DNA molecules are separated with Pulse Field Gel Electrophoresis (PFGE).