DNA Markers & Ladders

NEB offers a variety of DNA Ladders with sizes ranging from 10 bp to 48.5 kb and a wide range of conventional double-stranded DNA molecular weight markers ranging from 10 bp – 23 kb for agarose gel electrophoresis. Ladders are also available for pulsed-field gel electrophoresis. NEB’s DNA ladders are offered in several convenient formats:

Conventional (with no loading dye)
  • Available in a wide variety of size ranges
  • Most economical format
Quick-Load® Purple format
  • Ready-to load
  • Contains a non-fluorescing purple dye which does not cast a UV shadow
  • Optimized to create sharp, tight bands
  • Available for the Low Molecular Weight50 bp100 bp1 kb, and 1 kb Plus DNA Ladders.
  • Value pricing
Quick-Load format
  • Ready-to-load
  • Contains bromophenol blue dye
Tri-Dye® format

request a sample

 

The typical pattern generated by each of our markers and ladders is shown below. Fragment sizes are indicated alongside of the image. Relative mass values are also shown, when appropriate.

Most Popular DNA Ladders

50  bp

3% TBE agarose gel. Mass values are for 1 µg/lane.

50 bp DNA Ladder

Quick-Load® Purple 50 bp DNA Ladder

 

Low Molecular Weight

3% TBE agarose gel. Mass values are for 0.5 µg/lane.

Low Molecular Weight DNA Ladder

Quick-Load® Purple Low Molecular Weight DNA Ladder

 



Additional Ladders

Quick-Load® 1 kb Extend DNA Ladder

0.5% TBE agarose gel. Mass values are for 0.5 µg/lane.

Supercoiled DNA Ladder

0.8% TAE agarose gel. Mass values are for 0.5 µg/lane.

Fast DNA Ladder

1.2% TBE agarose gel. Mass values are for 0.5 µg/lane. 

***Can be used for fast electrophoresis systems as well as standard
electrophoresis

TriDye Ultra Low Range DNA Ladder

20% polyacrylamide gel. Mass values are for 0.5 µg/lane.

 

PCR Marker

1.8% TBE agarose gel. Mass values are for 0.3 µg/lane




Conventional DNA & PFG Markers

pBR322 DNA-MspI Digest

3% TBE agarose gel.

φX174 DNA – HaeIII Digest

1.7% TBE agarose gel. 

pBR322 DNA – BstNI Digest

1.4% TBE agarose gel. 

Lambda DNA – BstEII Digest

1.0% TBE agarose gel. 

Lambda DNA – HindIII Digest 

1.0% TBE agarose gel.

Lambda DNA – Mono Cut Mix

1% PFGE agarose gel.  

MidRange PFG Marker 

1% PFGE agarose gel

Lambda PFG Ladder

1% PFGE agarose gel.


UV Shadow Comparison


The new Gel Loading Dye, Purple (6X) (Lane 1) included in the Quick-Load Purple 1 kb DNA Ladder does not cast a UV shadow over the underlying bands, unlike the Gel Loading Dye, Blue (6X) (Lane 2).



Choose Type:

FAQs for DNA Markers & Ladders
Protocols for DNA Markers & Ladders
Advantages

  • DNA Ladders are supplied with 1 vial of Gel Loading Dye
  • Suitable for analysis of a wide range of DNAs
  • Uniform band intensities and convenient band spacing
  • Easy-to-identify reference bands
  • DNA Ladders can be used for sample quantitation
TriDye and Quick-Load Formats

  • TriDye™ Ladders: 1 kb, 100 bp and 1 kb Plus DNA Ladders are available in ready-to-load format containing xylene cyanol FF, bromophenol blue and orange G.
  • Quick-Load® Ladders: 1 kb, 1 kb extend, 50 bp, 100 bp, 1 kb Plus, Low Molecular Weight DNA Ladders and PCR marker are available in ready-to-load format containing either non-fluorescing purple dye or bromophenol blue as a tracking dye.
Comparison of NEB to Other Commercially Available DNA Ladders
Comparison of the NEB 1 kb (left) and 100 bp (right) DNA Ladders to other commercially available DNA Ladders. Note: The 500 bp DNA Ladder from Roche is used in the 1 kb DNA Ladder comparison.
DNA Markers & Ladders Selection Chart
Legal Information

This product is covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB).

While NEB develops and validates its products for various applications, the use of this product may require the buyer to obtain additional third party intellectual property rights for certain applications.

For more information about commercial rights, please contact NEB's Global Business Development team at [email protected].

This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.