DNA Markers & Ladders
NEB offers a variety of DNA Molecular Weight Markers and Ladders ranging from 10 bp all the way up to 1,018 kb. Ladders are available for agarose gel electrophoresis and pulsed-field gel electrophoresis.
Need help selecting a DNA marker or ladder?
Try our DNA Markers & Ladders Selection Tool.
Convenient DNA ladder formats
Conventional
(with no loading dye)
- Available in a wide variety of size ranges
- Most economical format
Quick-Load® Purple format
- Ready-to load
- Contains a non-fluorescing purple dye which does not cast a UV shadow
- Optimized to create sharp, tight bands
- Available for the Low Molecular Weight, 50 bp,
100 bp, 1 kb, and 1 kb Plus DNA Ladders. - Value pricing
Quick-Load format
- Ready-to-load
- Contains bromophenol blue dye
Tri-Dye® format
- Contains 3 dyes to monitor migration
- Available in Ultra Low Range, 100 bp, 1 kb and 1 kb Plus sizes
The typical pattern generated by each of our markers and ladders is shown below. Fragment sizes are indicated alongside of the image. Relative mass values are also shown, when appropriate.
Most Popular DNA Ladders
1 kb Plus
1.0% TBE agarose gel. Mass values are for 1 µg/lane.
Quick-Load® Purple 1 kb Plus DNA Ladder
50 bp
3% TBE agarose gel. Mass values are for 1 µg/lane.
Low Molecular Weight
3% TBE agarose gel. Mass values are for 0.5 µg/lane.
Additional Ladders
Quick-Load® 1 kb Extend DNA Ladder
0.5% TBE agarose gel. Mass values are for 0.5 µg/lane.
0.8% TAE agarose gel. Mass values are for 0.5 µg/lane.
1.2% TBE agarose gel. Mass values are for 0.5 µg/lane.
***Can be used for fast electrophoresis systems as well as standard
electrophoresis
1.8% TBE agarose gel. Mass values are for 0.3 µg/lane
Conventional DNA & PFG Markers
3% TBE agarose gel.
1.7% TBE agarose gel.
1.4% TBE agarose gel.
1.0% TBE agarose gel.
1.0% TBE agarose gel.
1% PFGE agarose gel.
1% PFGE agarose gel
1% PFGE agarose gel.
UV Shadow Comparison
The new Gel Loading Dye, Purple (6X) (Lane 1) included in the Quick-Load Purple 1 kb DNA Ladder does not cast a UV shadow over the underlying bands, unlike the Gel Loading Dye, Blue (6X) (Lane 2).
Choose Type:
- How can I quantify the amount of DNA in each band of a marker?
- What are the differences in the four versions of the 1 kb Plus (formerly called the 2-Log DNA Ladder), 100 bp and 1 kb DNA Ladders that NEB sells?
- Why are the DNA ladders showing up on my Southern blot? What is the sequence or composition of the ladder bands?
- Why are there extra bands visible on polyacrylamide gels?
- Where can I find sequence and restriction maps of NEB's expression vectors?
- PCR Reagents Brochure
- Protein Expression & Purification Brochure
Brochures
- DNA Ladders are supplied with 1 vial of Gel Loading Dye
- Suitable for analysis of a wide range of DNAs
- Uniform band intensities and convenient band spacing
- Easy-to-identify reference bands
- DNA Ladders can be used for sample quantitation
- TriDye™ Ladders: 1 kb, 100 bp and 1 kb Plus DNA Ladders are available in ready-to-load format containing xylene cyanol FF, bromophenol blue and orange G.
- Quick-Load® Ladders: 1 kb, 1 kb extend, 50 bp, 100 bp, 1 kb Plus, Low Molecular Weight DNA Ladders and PCR marker are available in ready-to-load format containing either non-fluorescing purple dye or bromophenol blue as a tracking dye.
Products and content are covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB). The use of trademark symbols does not necessarily indicate that the name is trademarked in the country where it is being read; it indicates where the content was originally developed. The use of this product may require the buyer to obtain additional third-party intellectual property rights for certain applications. For more information, please email busdev@neb.com.
This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.
