TriDye™ Ultra Low Range DNA Ladder is a pre-mixed, ready-to-load molecular weight marker containing three tracking dyes which serve as visual aids to monitor the progress of migration during native agarose and polyacrylamide gel electrophoresis.
The DNA ladder contains 13 bands ranging from 10 bp to 700 bp, most of which are generated from the digestion of a proprietary plasmid with restriction enzymes. The 50 bp and 300 bp bands have increased intensity to serve as reference bands. The ladder is suitable for use with for use with both native polyacrylamide and agarose gel electrophoresis.
Recommended gel percentage range: 4% TBE agarose, 10% to 20% TBE polyacrylamide
Migration of TriDye dyes during electrophoresis*
Gel
Xylene Cyanol
Bromophenol Blue
Orange G
4% Agarose
150 bp
20 bp
5 bp
5% Agarose
100 bp
15 bp
5 bp
6% PAGE
400 bp
50 bp
15 bp
10% PAGE
200 bp
30 bp
10 bp
4-20% PAGE
300 bp
20 bp
10 bp
20% PAGE
200 bp
20 bp
10 bp
*These migration rates are approximate and variations may occur based on migration conditions (TAE/TBE buffers, buffer pH, voltage etc.).
TriDye™ Ultra Low Range DNA Ladder visualized on a 4% TBE agarose gel and on a 20% TBE polyacrylamide gel. Mass values are for 0.5 µg/lane. Suggested Load: 5 to 10 µl/lane.
This product is related to the following categories:
Preparation of DNA:
The double-stranded DNA is digested to completion with appropriate restriction enzymes, phenol extracted and equilibrated in storage buffer.
Loading Recommendations:
For polyacrylamide gels, load 5 µl (0.5 µg) of TriDye™ Ultra Low Range DNA Ladder per gel lane.
For agarose gels, load 10 µl (1 µg) of TriDye™ Ultra Low Range DNA Ladder per gel lane.
Usage Recommendations:
When using the DNA ladder on agarose gels, we recommend using ethidium bromide at 0.5 ug/ml in both the gel and the migration buffer for proper visualization of the smallest DNA fragments. We also recommend that you do not cast gels exceeding 5mm in thickness, as the smaller DNA fragments might diffuse more easily and may be harder to efficiently stain.
We recommend using TBE buffer in the agarose gels and electrophoresis buffers, as TBE buffer allows for a better resolution of the smaller DNA fragments. Fragments below 15 bp might not separate efficiently when using TAE buffer.
We do not recommend using GelRed® or SYBR® dyes as precast gel stains, as these dyes might interfere with the migration of the smallest DNA fragments and result in an abnormal band pattern. We recommend using these dyes as post-electrophoresis stains only. Optimization might be required for proper visualization of all DNA fragments. Intensity of some bands might vary.
If using wide combs (10 mm) for agarose gel electrophoresis, we recommend loading 15 ul (1.5 ug) of TriDye™ Ultra Low Range DNA Ladder per gel lane for efficient visualization of the smallest DNA fragments.
For optimal visualization of all the bands, we recommend exposing the gel to UV without any casting tray whenever possible. Even UV-transparent trays can obscure DNA fragments under UV exposure.
Usage Notes:
When using the DNA ladder on agarose gels, we recommend using ethidium bromide at 0.5 ug/ml in both the gel and the migration buffer for proper visualization of the smallest DNA fragments. We also recommend that you do not cast gels exceeding 5mm in thickness, as the smaller DNA fragments might diffuse more easily and may be harder to efficiently stain.
We recommend using TBE buffer in the agarose gels and electrophoresis buffers, as TBE buffer allows for a better resolution of the smaller DNA fragments. Fragments below 15 bp might not separate efficiently when using TAE buffer.
We do not recommend using GelRed® or SYBR® dyes as precast gel stains, as these dyes might interfere with the migration of the smallest DNA fragments and result in an abnormal band pattern. We recommend using these dyes as post-electrophoresis stains only. Optimization might be required for proper visualization of all DNA fragments. Intensity of some bands might vary.
If using wide combs (10 mm) for agarose gel electrophoresis, we recommend loading 15 ul (1.5 ug) of TriDye™ Ultra Low Range DNA Ladder per gel lane for efficient visualization of the smallest DNA fragments.
For optimal visualization of all the bands, we recommend exposing the gel to UV without any casting tray whenever possible. Even UV-transparent trays can obscure DNA fragments under UV exposure.
Sambrook, J., Fritsch, E. F. and Maniatis, T. (1989). Molecular cloning: A Laboratory Manual, (2nd ed.). 10.51-10.67. Cold Spring Harbor Laboratory Press.
Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.
The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]
The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]
Products and content are covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB). The use of trademark symbols does not necessarily indicate that the name is trademarked in the country where it is being read; it indicates where the content was originally developed. The use of this product may require the buyer to obtain additional third-party intellectual property rights for certain applications. For more information, please email busdev@neb.com.
This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.
New England Biolabs (NEB) is committed to practicing ethical science – we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner. Learn more.
Ineligible item added to cart
Based on your Freezer Program type, you are trying to add a product to your cart that is either not allowed or not allowed with the existing contents of your cart. Please review and update your order accordingly If you have any questions, please contact Customer Service at freezers@neb.com or 1-800-632-5227 x 8.
You have been idle for more than 20 minutes, for your security you have been logged out. Please sign back in to continue your session.
Institution Changed
Your profile has been mapped to an Institution, please sign back for your profile updates to be completed.
Sign in to your NEB account
To save your cart and view previous orders, sign in to your NEB account. Adding products to your cart without being signed in will result in a loss of your cart when you do sign in or leave the site.
Powered by Bioz