Home Markers & Ladders Products Quick-Load® 1 kb Extend DNA Ladder

Quick-Load® 1 kb Extend DNA Ladder

  • Size range: 0.5 kb to 48.5 kb
  • Convenient, ready-to-load format
  • Formulated with bromophenol blue dye
  • Easy to identify reference bands
  • Suitable for 125 gel lanes

 

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Catalog # Concentration Size List Price Your Price Quantity
N3239S 50 µg/ml 125 gel lanes (1.25 ml) $83.00
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  • The Quick-Load 1 kb Extend DNA ladder is a pre-mixed, ready-to-load molecular weight DNA marker containing bromophenol blue dye as a tracking dye.

    The DNA Ladder consists of proprietary plasmids and DNA molecules which are digested to completion with appropriate restriction enzymes to yield 13 bands suitable for use as molecular weight standards for agarose gel electrophoresis. The digested DNA includes fragments ranging from 0.5 to 48.5 kilobases. The 3 kb fragment has increased intensity to serve as reference band.

    • Recommended gel and migration conditions: 0.5% 1X TBE agarose, 5V/cm (distance between electrodes), for 90 to 105 minutes

    N3239
    This product is related to the following categories:
    DNA Markers & Ladders Products
    This product can be used in the following applications:
    DNA Analysis

    • Reagents Supplied

      The following reagents are supplied with this product:

      NEB # Component Name Component # Stored at (°C) Amount Concentration

    • Properties & Usage

      Bases

      Fragment Mass (ng) bp
      1 30 48,502
      2a 15 20,066
      2b 15 19,871
      3 45 15,000
      4 33 10,002
      5 33 8,001
      6 40 6,001
      7 33 5,001
      8 26 4,001
      9 100 3,001
      10 37 2,000
      11 27 1,500
      12 33 1,000
      13a 18 517
      13b 17 500

      Preparation

      The double-stranded DNA is digested to completion with appropriate restriction enzymes, phenol extracted and equilibrated in storage buffer.

      Effective Size Range

      500bp to 48,502bp

      Storage Buffer

      2.5% Ficoll®-400
      11 mM EDTA
      3.3 mM Tris-HCl
      0.017% SDS
      0.015% bromophenol blue
      pH 8 @ 25°C

    • Related Products

      Companion Products

      Materials Sold Separately

    • Product Notes

      1. Quick-Load 1 kb Extend DNA Ladder is stable for at least 6 months at 25°C.

        Store at 4°C. Do not store at -20°C.
      2. Usage Recommendation: We recommend loading 10 μl (0.5 μg) of Quick-Load 1 kb Extend DNA Ladder per gel lane. For optimum separation of the highest molecular weight DNA fragments, run on a 0.6% (1X TAE or 1X TBE) agarose gel at 3.5 V/cm (distance between electrodes) for 3 hours.

        In general, it is recommended to load 1 ul per mm of gel lane.

        The Quick-Load 1 kb Extend DNA Ladder is not intended for precise quantification of DNA mass but can be used for approximating the mass of DNA in comparably intense samples of similar size.

    • References

      1. Sambrook, J., Fritsch, E. F. and Maniatis, T. (1989). Cold Spring Harbor: Cold Spring Harbor Laboratory Press.(Ed.), Molecular Cloning: A Laboratory Manual. pp. 10.51-10.67.

  • Protocols, Manuals & Usage

  • FAQs & Troubleshooting

  • Citations & Technical Literature

  • Quality, Safety & Legal

    • Quality Control Assays

      Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.

    • Specifications & Change Notifications

      The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]

    • Certificate of Analysis

      The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]

    • Safety Data Sheets

      The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.

    • Legal and Disclaimers

      Products and content are covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB). The use of trademark symbols does not necessarily indicate that the name is trademarked in the country where it is being read; it indicates where the content was originally developed. The use of this product may require the buyer to obtain additional third-party intellectual property rights for certain applications. For more information, please email busdev@neb.com.

      This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

      New England Biolabs (NEB) is committed to practicing ethical science – we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner. Learn more.

      Trademarks

      QUICK-LOAD® is a registered trademark of New England Biolabs, Inc.
      FICOLL® is a registered trademark of GE Healthcare Bio-Sciences AB.

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