TriDye™ 1 kb DNA Ladder

The following reagents are supplied with this product:

NEB #	Component Name	Component #	Stored at (°C)	Amount	Concentration
N3272S     4       TriDye™ 1 kb DNA Ladder N3272SVIAL 4 1 x 1.25 ml 50 µg/ml

Companion Products

• 1 kb DNA Ladder
• 100 bp DNA Ladder
• 50 bp DNA Ladder
• PCR Marker
• Quick-Load® 1 kb DNA Ladder
• Quick-Load® 100 bp DNA Ladder
• Quick-Load^® 1 kb Plus DNA Ladder
• TriDye™ 100 bp DNA Ladder
• TriDye™ 1 kb Plus DNA Ladder
• Monarch® Plasmid Miniprep Kit 
• Monarch® DNA Gel Extraction Kit
• Monarch® PCR & DNA Cleanup Kit (5 μg)

1. We recommend loading 10 μl (0.5 μg) of TriDye DNA Ladder per gel lane.
2. In general, it is recommended to load 1ul per mm of gel lane.
3. The TriDye DNA Ladder was not designed for precise quantification of DNA mass but can be used for approximating the mass of DNA in comparably intense samples of similar size.
4. TriDye DNA Ladder is stable for at least 6 months at 25°C.
5. For long term storage, store at 4°C or -20°C. If stored at -20°C, mix well after thawing.

1. Sambrook, J., Fritsch, E.F. and Maniatis, T. (1989). Molecular Cloning: A Laboratory Manual (2nd Ed.). 10.51-10.67.

1. Protocol for TriDye 1 kb DNA Ladder (NEB #N3272)

• DNA Markers & Ladders Selection Tool

1. Why are the DNA ladders showing up on my Southern blot? What is the sequence or composition of the ladder bands?
2. How can I quantify the amount of DNA in each band of a marker?
3. Can I use Midori Green with the DNA Ladders from NEB?
4. Can I use SYBR^® and/or GelRed^® dyes with the DNA Ladders from NEB?
5. Why is my DNA Ladder floating out the wells and Why are there no bands visible in my DNA ladder gel lane?
6. What are the differences in the four versions of 2-Log, 100 bp and 1 kb DNA Ladders that NEB sells?

Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.

The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]

• N3272S_v2

The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]

• N3272S_v1_0121401
• N3272S_v1_0121407
• N3272S_v1_0121412
• N3272S_v1_0131505
• N3272S_v1_0131511
• N3272S_v1_0131604
• N3272S_v1_0141608
• N3272S_v1_0141612
• N3272S_v1_0141704
• N3272S_v1_0151711
• N3272S_v1_10021818
• N3272S_v1_10045824
• N3272S_v2_10060578
• N3272S_v2_10092833
• N3272S_v2_10111564
• N3272S_v2_10137431
• N3272S_v2_10176563
• N3272S_v2_10209948
• N3272S_v2_10251324

The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.

• TriDye™ 1 kb DNA Ladder

Products and content are covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB). The use of trademark symbols does not necessarily indicate that the name is trademarked in the country where it is being read; it indicates where the content was originally developed. The use of this product may require the buyer to obtain additional third-party intellectual property rights for certain applications. For more information, please email busdev@neb.com.

This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

New England Biolabs (NEB) is committed to practicing ethical science – we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner. Learn more.