TriDye™ 100 bp DNA Ladder

Description

TriDye™ 100 bp DNA Ladder is a pre-mixed, ready-to-load molecular weight marker containing 3 dyes which serve as visual aids to monitor the progress of migration during agarose gel electrophoresis.

The DNA ladder consists of proprietary plasmids are digested to completion with appropriate restriction enzymes to yield 12 bands suitable for use as molecular weight standards for agarose gel electrophoresis. The digested DNA includes fragments ranging from 100-1,517 base pairs. The 500 and 1,000 base pair bands have increased intensity to serve as reference points.

TriDye During Electrophoresis: On a standard 1% agarose gel in 1X TBE, xylene cyanol FF migrates at approximately 4 kb, bromophenol blue at approximately 300 bp and the orange G at approximately 50 bp. As the percentage of agarose changes, the migration rates of the dyes relative to migration rates of the DNA will change.


N3271fig2_thumb

100 bp DNA Ladder visualized by ethidium bromide staining on a 1.3% TAE agarose gel. Mass values are for 0.5 µg/lane

Properties and Usage

Bases

FragmentMassbp
1451,517
2351,200
3951,000
427900
524800
621700
718600
897500/517
938400
1029300
1125200
1248100

Effective Size Range

100bp to 1,517bp

Storage Temperature

4°C

Storage Conditions

0.006% Xylene Cyanol
10 mM Tris-HCl
10 mM EDTA
10% Glycerol
0.006% bromophenol blue
0.06% Orange G
pH 7.9 @ 25°C

Notes

  1. The TriDye DNA Ladder was not designed for precise quantification of DNA mass but can be used for approximating the mass of DNA in comparably intense samples of similar size.
  2. We recommend loading 10 μl (0.5 μg) of TriDye DNA Ladder per gel lane.
  3. In general, it is recommended to load 1ul per mm of gel lane.
  4. TriDye DNA Ladder is stable for at least 6 months at 25°C
  5. For long term storage, store at 4°C or -20°C. If stored at -20°C, mix well after thawing.

References

  1. Sambrook, J., Fritsch, E.F. and Maniatis, T. (1989). Molecular Cloning: A Laboratory Manual (2nd Ed.). 10.51-10.67.

FAQs

  1. Why are the DNA ladders showing up on my Southern blot? What is the sequence or composition of the ladder bands?
  2. How can I quantify the amount of DNA in each band of a marker?
  3. Why are there extra bands visible on polyacrylamide gels?
  4. What are the differences in the four versions of 2-Log, 100 bp and 1 kb DNA Ladders that NEB sells?
  5. Can I use GelRed with the DNA Ladders from NEB?
  6. Can I use Midori Green with the DNA Ladders from NEB?
  7. Can I use SYBR® with the DNA Ladders from NEB?

Tech Tips

Mix well before use, especially after thawing.

Datacards

The Product Summary Sheet, or Data Card, includes details for how to use the product, as well as details of its formulation and quality controls. The following file naming structure is used to name the majority of these document files: [Catalog Number]Datasheet-Lot[Lot Number]. For those product lots not listed below, please contact NEB at info@neb.com or fill out the Technical Support Form for appropriate document.

Selection Charts

Interactive Tools

Certificate of Analysis

The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality control's for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]

Specifications

The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]

Safety Data Sheet

The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.

Datacards

The Product Summary Sheet, or Data Card, includes details for how to use the product, as well as details of its formulation and quality controls. The following file naming structure is used to name the majority of these document files: [Catalog Number]Datasheet-Lot[Lot Number]. For those product lots not listed below, please contact NEB at info@neb.com or fill out the Technical Support Form for appropriate document.