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  • TriDye™ 100 bp DNA Ladder

    Description

    TriDye™ 100 bp DNA Ladder is a pre-mixed, ready-to-load molecular weight marker containing 3 dyes which serve as visual aids to monitor the progress of migration during agarose gel electrophoresis.

    The DNA ladder consists of proprietary plasmids are digested to completion with appropriate restriction enzymes to yield 12 bands suitable for use as molecular weight standards for agarose gel electrophoresis. The digested DNA includes fragments ranging from 100-1,517 base pairs. The 500 and 1,000 base pair bands have increased intensity to serve as reference points.

    TriDye During Electrophoresis: On a standard 1% agarose gel in 1X TBE, xylene cyanol FF migrates at approximately 4 kb, bromophenol blue at approximately 300 bp and the orange G at approximately 50 bp. As the percentage of agarose changes, the migration rates of the dyes relative to migration rates of the DNA will change.


    N3271fig2_thumb

    100 bp DNA Ladder visualized by ethidium bromide staining on a 1.3% TAE agarose gel. Mass values are for 0.5 µg/lane

    Properties and Usage

    Bases

    FragmentMassbp
    1451,517
    2351,200
    3951,000
    427900
    524800
    621700
    718600
    897500/517
    938400
    1029300
    1125200
    1248100

    Effective Size Range

    100bp to 1,517bp

    Storage Temperature

    4°C

    Storage Conditions

    0.006% Xylene Cyanol
    10 mM Tris-HCl
    10 mM EDTA
    10% Glycerol
    0.006% bromophenol blue
    0.06% Orange G
    pH 7.9 @ 25°C

    Notes

    1. The TriDye DNA Ladder was not designed for precise quantification of DNA mass but can be used for approximating the mass of DNA in comparably intense samples of similar size.
    2. We recommend loading 10 μl (0.5 μg) of TriDye DNA Ladder per gel lane.
    3. In general, it is recommended to load 1ul per mm of gel lane.
    4. TriDye DNA Ladder is stable for at least 6 months at 25°C
    5. For long term storage, store at 4°C or -20°C. If stored at -20°C, mix well after thawing.

    References

    1. Sambrook, J., Fritsch, E.F. and Maniatis, T. (1989). Molecular Cloning: A Laboratory Manual (2nd Ed.). 10.51-10.67.

    FAQs

    1. Why are the DNA ladders showing up on my Southern blot? What is the sequence or composition of the ladder bands?
    2. How can I quantify the amount of DNA in each band of a marker?
    3. Why are there extra bands visible on polyacrylamide gels?
    4. What are the differences in the four versions of 2-Log, 100 bp and 1 kb DNA Ladders that NEB sells?
    5. Can I use GelRed with the DNA Ladders from NEB?
    6. Can I use Midori Green with the DNA Ladders from NEB?
    7. Can I use SYBR® with the DNA Ladders from NEB?

    Tech Tips

    Mix well before use, especially after thawing.

    Datacards

    The Product Summary Sheet, or Data Card, includes details for how to use the product, as well as details of its formulation and quality controls. The following file naming structure is used to name the majority of these document files: [Catalog Number]Datasheet-Lot[Lot Number]. For those product lots not listed below, please contact NEB at info@neb.com or fill out the Technical Support Form for appropriate document.

    Selection Tools

    Interactive Tools

    Certificate of Analysis

    The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality control's for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]

    Specifications

    The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]

    Safety Data Sheet

    The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.

    Datacards

    The Product Summary Sheet, or Data Card, includes details for how to use the product, as well as details of its formulation and quality controls. The following file naming structure is used to name the majority of these document files: [Catalog Number]Datasheet-Lot[Lot Number]. For those product lots not listed below, please contact NEB at info@neb.com or fill out the Technical Support Form for appropriate document.