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Home Markers & Ladders Products TriDye™ 100 bp DNA Ladder

TriDye™ 100 bp DNA Ladder

Size range: 100 bp to 1,517 bp
Convenient, ready-to-load format
Formulated with 3 different tracking dyes
Easy to identify reference bands
Suitable for 125 gel lanes

Catalog #	Concentration	Size	List Price	Your Price
N3271S	50 µg/ml	125 gel lanes (1.25 ml)	$101.00	Sign In Sign In

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Product Information
TriDye™ 100 bp DNA Ladder is a pre-mixed, ready-to-load molecular weight marker containing 3 dyes which serve as visual aids to monitor the progress of migration during agarose gel electrophoresis.

The DNA ladder consists of proprietary plasmids are digested to completion with appropriate restriction enzymes to yield 12 bands suitable for use as molecular weight standards for agarose gel electrophoresis. The digested DNA includes fragments ranging from 100-1,517 base pairs. The 500 and 1,000 base pair bands have increased intensity to serve as reference points.
- Recommended gel percentage range: 1.2-3%
- Optimum separation on 2%
TriDye During Electrophoresis: On a standard 1% agarose gel in 1X TBE, xylene cyanol FF migrates at approximately 4 kb, bromophenol blue at approximately 300 bp and the orange G at approximately 50 bp. As the percentage of agarose changes, the migration rates of the dyes relative to migration rates of the DNA will change.

100 bp DNA Ladder visualized by ethidium bromide staining on a 1.3% TAE agarose gel. Mass values are for 0.5 µg/lane

This product is related to the following categories:

DNA Markers & Ladders Products

This product can be used in the following applications:

DNA Analysis
- Reagents Supplied
  Reagents Supplied
  
  The following reagents are supplied with this product:
  
  NEB # Component Name Component # Stored at (°C) Amount Concentration
  
  N3271S 4
  
  TriDye™ 100 bp DNA Ladder N3271SVIAL 4 1 x 1.25 ml 50 µg/ml
- Properties & Usage
  
  Bases
  
  Fragment Mass (ng) bp
  
  1 45 1,517
  
  2 35 1,200
  
  3 95 1,000
  
  4 27 900
  
  5 24 800
  
  6 21 700
  
  7 18 600
  
  8 97 500/517
  
  9 38 400
  
  10 29 300
  
  11 25 200
  
  12 48 100
  
  Effective Size Range
  100bp to 1,517bp
  Storage Buffer
  
  0.006% Xylene Cyanol
  10 mM Tris-HCl
  10 mM EDTA
  10% Glycerol
  0.006% bromophenol blue
  0.06% Orange G
  pH 8 @ 25°C
- Related Products
  Companion Products
  
  1 kb DNA Ladder
  
  100 bp DNA Ladder
  
  50 bp DNA Ladder
  
  PCR Marker
  
  Quick-Load® 1 kb DNA Ladder
  
  Quick-Load® 100 bp DNA Ladder
  
  Quick-Load^® 1 kb Plus DNA Ladder
  
  TriDye™ 1 kb DNA Ladder
  
  TriDye™ 1 kb Plus DNA Ladder
  
  Monarch® Plasmid Miniprep Kit
  
  Monarch® DNA Gel Extraction Kit
  
  Monarch® PCR & DNA Cleanup Kit (5 μg)
- Product Notes
  The TriDye DNA Ladder was not designed for precise quantification of DNA mass but can be used for approximating the mass of DNA in comparably intense samples of similar size.
  
  We recommend loading 10 μl (0.5 μg) of TriDye DNA Ladder per gel lane.
  
  In general, it is recommended to load 1ul per mm of gel lane.
  
  TriDye DNA Ladder is stable for at least 6 months at 25°C
  
  For long term storage, store at 4°C or -20°C. If stored at -20°C, mix well after thawing.
- References
  Sambrook, J., Fritsch, E.F. and Maniatis, T. (1989). Molecular Cloning: A Laboratory Manual (2nd Ed.). 10.51-10.67.
Protocols, Manuals & Usage
- Protocols
  Protocol for TriDye 100 bp DNA Ladder (NEB #N3271)
Tools & Resources
- Selection Charts
  DNA Markers & Ladders Selection Chart
FAQs & Troubleshooting
- FAQs
  Why are the DNA ladders showing up on my Southern blot? What is the sequence or composition of the ladder bands?
  
  How can I quantify the amount of DNA in each band of a marker?
  
  Why are there extra bands visible on polyacrylamide gels?
  
  What are the differences in the four versions of 2-Log, 100 bp and 1 kb DNA Ladders that NEB sells?
  
  Can I use Midori Green with the DNA Ladders from NEB?
  
  Can I use SYBR^® and/or GelRed^® dyes with the DNA Ladders from NEB?
  
  Why is my DNA Ladder floating out the wells and Why are there no bands visible in my DNA ladder gel lane?
- Tech Tips
  
  Mix well before use, especially after thawing.
Citations & Technical Literature
- Citations
  
  Product Citation Tool
  
  Powered by Bioz See more details on Bioz
Quality, Safety & Legal
- Quality Control Assays
  
  Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.
- Specifications & Change Notifications
  Specifications & Change Notifications
  The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]
  
  N3271S_v2
- Certificate of Analysis
  The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]
  
  N3271S_v1_0161401
  
  N3271S_v1_0161407
  
  N3271S_v1_0171505
  
  N3271S_v1_0181511
  
  N3271S_v1_0191608
  
  N3271S_v1_0191612
  
  N3271S_v1_0201704
  
  N3271S_v1_0201712
  
  N3271S_v1_10013009
  
  N3271S_v1_10025579
  
  N3271S_v1_10045822
  
  N3271S_v2_10064297
  
  N3271S_v2_10082430
  
  N3271S_v2_10097407
  
  N3271S_v2_10123581
  
  N3271S_v2_10150224
  
  N3271S_v2_10171175
- Safety Data Sheets
  The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.
  
  TriDye™ 100 bp DNA Ladder
- Legal and Disclaimers
  
  Products and content are covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB). The use of trademark symbols does not necessarily indicate that the name is trademarked in the country where it is being read; it indicates where the content was originally developed. The use of this product may require the buyer to obtain additional third-party intellectual property rights for certain applications. For more information, please email busdev@neb.com.
  
  This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.
  
  New England Biolabs (NEB) is committed to practicing ethical science – we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner. Learn more.