Hi-T7^® RNA Polymerase

Reagents Supplied

The following reagents are supplied with this product:

NEB #	Component Name	Component #	Stored at (°C)	Amount	Concentration
M0658S     -20       Hi-T7® RNA Polymerase M0658SVIAL -20 1 x 0.1 ml 50,000 units/ml   Hi-T7® RNA Polymerase Reaction Buffer B0658AVIAL -20 1 x 1 ml Not Applicable

Application Features

• Radiolabeled RNA probes
• Non-isotopic RNA labeling
• Guide RNA for gene targeting
• mRNA for in vitro translation, microinjection and transfection 
• RNA structure, processing and catalysis studies
• RNA amplification
• Isothermal amplification
• Anti-sense RNA for gene expression

Companion Products

• T7 RNA Polymerase
• RNase Inhibitor, Murine
• Ribonucleotide Solution Set
• Ribonucleotide Solution Mix

1. Protocol for Standard RNA Synthesis with Hi-T7 RNA Polymerase (NEB #M0658)

1. Can I use a different reaction buffer for in vitro transcription with Hi-T7 RNA Polymerase?
2. Does Hi-T7 RNA Polymerase recognize the same promoter sequence as the wild-type T7 RNA Polymerase from bacteriophage?
3. At what temperature is Hi-T7 RNA Polymerase active?
4. What are the main causes of reaction failure when using Hi-T7 RNA Polymerase?
5. What templates can be used for in vitro transcription with Hi-T7 RNA Polymerase?
6. Can Hi-T7 RNA Polymerase transcribe off of an uncut plasmid?

Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.

Specifications & Change Notifications

The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]

• M0658S_v1

The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]

• M0658S_v1_10014907
• M0658S_v1_10042254
• M0658S_v1_10060569
• M0658S_v1_10065550
• M0658S_v1_10076188
• M0658S_v1_10077401
• M0658S_v1_10103413
• M0658S_v1_10115580
• M0658S_v1_10121442
• M0658S_v1_10132825
• M0658S_v1_10160994
• M0658S_v1_10182661
• M0658S_v1_10183471

The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.

• Hi-T7^® RNA Polymerase
• Hi-T7^® RNA Polymerase Reaction Buffer

Products and content are covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB). The use of trademark symbols does not necessarily indicate that the name is trademarked in the country where it is being read; it indicates where the content was originally developed. The use of this product may require the buyer to obtain additional third-party intellectual property rights for certain applications. For more information, please email busdev@neb.com.

This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

New England Biolabs (NEB) is committed to practicing ethical science – we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner. Learn more.