Hi-T7™ RNA Polymerase
Hi-T7 RNA Polymerase is designed for in vitro transcription of RNA at higher temperatures.
- Supplied with 10X Hi-T7 RNA Polymerase Reaction Buffer formulated for optimal performance
- Active from 37-56°C
- For standard in vitro transcription at 37°C use T7 RNA Polymerase (NEB# M0251)
- Increased co-transcriptional capping efficiency with cap analogs
- Decreased immunogenicity from RNA synthesized at higher temperature due to reduced dsRNA by-product formation
- Interested in formulations for high-yield reactions? Contact us at [email protected]
Hi-T7 RNA Polymerase is an engineered DNA-dependent RNA polymerase that is highly specific for T7 phage promoters and is designed to function at higher temperatures than the wild-type bacteriophage T7 RNA Polymerase. Hi-T7 RNA Polymerase functions at an optimal temperature of 50-52°C.
Product SourceAn E. coli strain that carries a plasmid encoding the engineered T7 RNA Polymerase gene.
The following reagents are supplied with this product:
Store at (°C) Concentration Hi-T7™ RNA Polymerase Reaction Buffer -20 10 X
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