HiFi Taq DNA Ligase
An optimized blend of a thermostable DNA ligase and a proprietary additive, HiFi Taq DNA Ligase efficiently seals nicks in DNA with unmatched fidelity.
- Reduces errors for ligation-based molecular diagnostic techniques (e.g., LCR, LDR)
- Remains active after multiple thermal cycles
- Exhibits increased discrimination between correct and mismatched base pairs at either side of ligation junctions, making the enzyme suitable for the detection of either 3 ́ or 5 ́ mismatches (vs. other thermostable ligases which are efficient for 3 ́ mismatches only).
- Not sure which ligase to choose? Refer to our DNA and RNA Ligase Properties Chart
DNA Ligase Fidelity: when does it matter?
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An optimized blend of a thermostable DNA Ligase and a proprietary additive, HiFi Taq DNA Ligase efficiently seals nicks in DNA with unmatched high fidelity. The formation of a phosphodiester bond between juxtaposed 5´ phosphate and 3´ hydroxyl termini of two adjacent oligonucleotides that are hybridized to a complementary target DNA is enhanced in the improved reaction buffer and mismatch ligation is dramatically reduced (1). The improved formulation allows higher resolution discrimination between ligation donors and acceptors, enabling precise detection of SNPs and other allele variants. HiFi Taq DNA Ligase is active at elevated temperatures (37–75°C) (2,3).
Please note that HiFi Taq DNA Ligase is intended for use in molecular diagnostics applications that depend on high fidelity nick ligation. It is not a substitute for T4 DNA ligase and is not suitable for cloning applications or adapter ligation/NGS library prep.
Figure 1: HiFi Taq DNA Ligase displays increased fidelity
Figure 2: HiFi Taq DNA Ligase exhibits increased discrimination at both sides of the ligation junction
Figure 3: HiFi Taq DNA Ligase exhibits increased thermostability
Figure 4: HiFi Taq DNA Ligase displays 2-fold reduction in mismatch ligation as compared to Ampligase
Product SourcePurified from an E. coli strain that carries the cloned ligase gene from a hyperthermophilic organism.
The following reagents are supplied with this product:
Store at (°C) Concentration HiFi Taq DNA Ligase Reaction Buffer -20 10 X
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