FAQ: Can HiFi Taq DNA Ligase be used in other buffers?  

In order to achieve the highest fidelity possible with HiFi Taq DNA Ligase, we recommend using HiFi Taq DNA Ligase buffer, however, HiFi Taq DNA Ligase can be used in Taq DNA Ligase buffer with reduced activity and fidelity. HiFi Taq DNA Ligase can be used in a variety of polymerase buffers when supplemented with 1 mM NAD with reduced activity and modestly reduced fidelity.

 NEB Buffer  HiFi Taq DNA Ligase Activity (%) Effect on Fidelity
 Hifi Taq DNA Ligase Buffer
 100  -
 Taq DNA Ligase Buffer
 38  ↓↓↓
 phi29 Reaction Buffer1
 54  ↓↓
 Q5® Reaction Buffer1
 73  ↓
 Standard Taq Reaction Buffer1
 90  ↓
 ThermoPol Buffer®1
 215  ↓↓

1Supplemented with 1 mM NAD.

2Additional supplementation of polymerase buffers with 5 mM Mg may increase HiFi Taq DNA Ligase activity, but further reduce fidelity.