While more than one ligase may work for your application, the following selection chart presents our recommendations for optimal performance. To select a ligase based upon the type of substrate present, please visit Substrate-based Ligase Selection Chart. In addition, the Properties of DNA and RNA Ligaseswill help you understand the differences between them.
Ligation-Dependent DNA Sequence & SNP Detection (LCR, LDR & related methods)
★★★
★★
★★
Ligation-Dependent RNA Sequence & SNP Detection
★
★★★
Ligation of adjacent ssDNAs on an RNA splint
★★★
NGS APPLICATIONS
NGS Library Prep dsDNA-dsDNA (ligation)
▲
▲
▲
▲
FEATURES
Salt tolerance
(>2X that for T4 DNA Ligase)
✓
✓
Ligation in 15 min. or less
✓
✓
✓
✓
✓
✓
✓
✓
✓
✓
✓
✓
✓
Master Mix Formulation
✓
✓
✓
✓
Thermostable
✓
✓
✓
Thermotolerant
✓
✓
✓
✓
Recombinant
✓
✓
✓
✓
✓
✓
✓
✓
✓
✓
✓
✓
✓
✓
✓
✓
✓
Reusable/Removable
✓
KEY
★★★
Optimal, recommended
ligase for selected
application
★★
Works well for
selected
application
★
Will perform selected
application, but is not
recommended
▲
Please consult the specific
NGS protocol to determine the
optimal enzyme for your needs
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