HomeFAQsDo thermostable DNA ligases (such as Taq DNA Ligase, 9°N DNA Ligase, and HiFi Taq DNA Ligase) ligate sticky ends?
FAQ: Do thermostable DNA ligases (such as Taq DNA Ligase, 9°N DNA Ligase, and HiFi Taq DNA Ligase) ligate sticky ends?
While these thermostable ligases can join sticky ends with extensive overlap, like the 12 bp cohesive ends of the genome of bacteriophage Lambda, typical 4 bp overhangs generated by Type II restriction enzymes are not good substrates and will not be ligated. Thermostable DNA ligases are not recommended for use in traditional cloning workflows.
Choose your country
North America
Europe
Asia-Pacific
Session Expired
You have been idle for more than 20 minutes, for your security you have been logged out. Please sign back in to continue your session.
Institution Changed
Your profile has been mapped to an Institution, please sign back for your profile updates to be completed.
Sign in to your NEB account
To save your cart and view previous orders, sign in to your NEB account. Adding products to your cart without being signed in will result in a loss of your cart when you do sign in or leave the site.
