HomeFAQsHow much HiFi Taq DNA Ligase is required for a reaction?
FAQ: How much HiFi Taq DNA Ligase is required for a reaction?
A typical reaction uses 1 μL of HiFi Taq DNA Ligase in a 50 μL reaction volume. Please see the accompanying product information for details on reaction conditions including molar ratios or probes, target DNA, and enzyme.
This video provides tips for calculating the ideal insert:vector molar ratio before performing a ligation reaction.
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