T7 RNA Polymerase
T7 RNA Polymerase is used for in vitro mRNA synthesis and is highly specific for the T7 phage promoter. Applications include:
- Radiolabeled RNA probe preparation
- RNA generation for studies of RNA structure, processing and catalysis
- Expression control via anti-sense RNA
Avoiding RNase Contamination
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Bacteriophage T7 RNA Polymerase is a DNA-dependent RNA polymerase that is highly specific for the T7 phage promoters. The 99 KD enzyme catalyzes in vitro RNA synthesis from a cloned DNA sequence under the T7 promoters. RNA produced using the T7 RNA Polymerase is suitable for many applications in research and biotechnology.
1X RNAPol Reaction Buffer, supplemented with 0.5 mM each ATP, UTP, GTP, CTP, and DNA template containing the T7 RNA Polymerase promoter. Incubate at 37°C.
Product SourceIsolated from E.coli carrying a plasmid which contains the T7 RNA Polymerase gene.
The following reagents are supplied with this product:
Store at (°C) Concentration RNAPol Reaction Buffer -20 10 X
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