GMP-grade Products for RNA Synthesis – Tools to Take you from Template to Transcript
For almost 50 years, NEB® has been a world leader in the discovery and production of reagents for the life science industry. Our enzymology expertise effectively positions us to supply reagents for the synthesis of high-quality RNA – from template generation and transcription, to capping, tailing and cleanup after synthesis. These products are designed and manufactured based on decades of molecular biology experience, so that you can be confident they will work for your application.
From research to therapeutic production, NEB’s in vitro transcription portfolio will meet your needs
NEB’s portfolio of research-grade and GMP-grade* products enables bench-scale to commercial-scale mRNA manufacturing. Our optimized HiScribe® kits enable convenient in vitro transcription (IVT) workflows. When it is time to scale up and optimize reaction components, our standalone reagents are readily available in formats matching our GMP-grade offering, enabling a seamless transition to large-scale therapeutic mRNA manufacturing.
Enabling gram-scale RNA synthesis
NEB manufactures and inventories the following enzyme specificities at GMP-grade, meeting customer needs with short lead times:
| IVT Workflow | Product Name | Product Description | Note |
|---|---|---|---|
| Template Generation | Q5® Hot Start High-Fidelity DNA Polymerase (NEB #M0493) | Composed of a novel polymerase that is fused to the processivity-enhancing Sso7d DNA binding domain, improving speed, fidelity and reliability of performance | The GMP-grade reagent has a different formulation than the commercially-available product. Learn more by contacting our Customized Solutions team. |
| phi29 DNA Polymerase (NEB #M0269) | Replicative polymerase from the Bacillus subtillis phage phi29 and has exceptional strand displacement and processive synthesis properties with inherent 3´→ 5´ proofreading exonuclease activity | The GMP-grade reagent has a different formulation than the commercially-available product. Learn more by contacting our Customized Solutions team. | |
| TelN Protelomerase (NEB #M0651) | Cuts dsDNA at the TelN recognition sequence (56 bp) and leaves covalently closed ends at the site of cleavage | ||
| Deoxynucleotide (dNTP) Solution Mix (NEB #N0447) | An equimolar solution of ultrapure dATP, dGTP, dCTP, and dTTP | ||
| BspQI (NEB #R0712) | Type IIS restriction enzyme and isoschizomer of LguI and SapI used to linearize plasmid DNA for mRNA therapeutics | ||
| BsaI-HF®v2 (NEB #R3733) | Type IIS restriction enzyme used in Golden Gate assembly and linearization of plasmid DNA for mRNA therapeutics |
The GMP-grade reagent has a different formulation than the commercially-available product. Learn more by contacting our Customized Solutions team.
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| XbaI (NEB #R0145) | Restriction enzyme used to linearize plasmid DNA for mRNA therapeutics |
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| In vitro Transcription (IVT) | HiScribe T7 High Yield RNA Synthesis Components (NEB #E2040) | Separate components availble in GMP-grade formats | |
| T7 RNA Polymerase (NEB #M0251) | RNA polymerase used for in vitro mRNA synthesis, and is highly specific for the T7 phage promoter | ||
| IVT Compantion Products | RNase Inhibitor, Murine (NEB #M0314) | Specifically inhibits RNases A, B, and C | |
| Pyrophosphatase, Inorganic (E. coli) (NEB #M0361) | Catalyzes the hydrolysis of inorganic pyrophosphate to form orthophosphate | ||
| DNase I (RNase-free) (NEB #M0303) | A specific endonuclease used for removal of contaminating genomic DNA from RNA samples and degradation of DNA templates in transcription reactions | ||
| NTPs (NEB #N0450) | Individual NTPs available in GMP-grade format | ||
| RNA Capping | Faustovirus Capping Enzyme (NEB #M2081) | Adds the m7G-cap (Cap-0) to the 5´ end of the triphosphorylated and dephosphorylated RNA | |
| Vaccinia Capping Enzyme (NEB #M2080) | Adds the m7G-cap (Cap-0) to the 5' end of the triphosphorylated and dephosphorylated RNA | ||
| mRNA Cap 2´-O-Methyltransferase (NEB #M0366) | Adds a methyl group at the 2´-O position of the first nucleotide adjacent to the cap structure at the 5´ end of the RNA | ||
| S-adenosylmethionine (SAM) (NEB #B9003) | Commonly used cofactor in enzymatic methylation reactions of nucleic acids, proteins, lipids, and secondary metabolites |
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Not sure whether you need research-grade or GMP-grade products? Learn more about the difference between GMP-grade and research-grade products |
= available in GMP-grade
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| Functional expression of active green fluorescent protein in HEK 293 cells after transfection of capped poly-adenylated mRNA demonstrates qualitative equivalence of GMP-grade and research-grade reagents. |
* “GMP Grade” and “GMP-grade” are branding terms NEB uses to describe products manufactured or finished at NEB’s Rowley facility. The Rowley facility was designed to manufacture products under more rigorous infrastructure and process controls to achieve more stringent product specifications and customer requirements. Products manufactured at NEB’s Rowley facility are manufactured in compliance with ISO 9001 and ISO 13485 quality management system standards. However, at this time, NEB does not manufacture or sell products known as Active Pharmaceutical Ingredients (APIs), nor does NEB manufacture its products in compliance with all of the Current Good Manufacturing Practice regulations.
Products and content are covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB). The use of trademark symbols does not necessarily indicate that the name is trademarked in the country where it is being read; it indicates where the content was originally developed. All other trademarks are the property of their respective owners. The use of this product may require the buyer to obtain additional third-party intellectual property rights for certain applications. For more information, please email busdev@neb.com.
