For details on how to combine UMI adaptors with our NEBNext library prep kits, click the product information tab below.
NEBNext® Multiplex Oligos for Illumina® are an essential piece of the NEBNext suite of library preparation products, available in several different configurations for use with NEBNext products or other standard Illumina-compatible library preparation protocols. This set was designed and optimized for use in DNA sequencing workflows, including PCR-free. These unique dual index adaptors also include a unique molecular identifier (UMI) sequence to enable the identification and removal of PCR errors or duplicates from amplified libraries. Supplied as 96 unique, pre-annealed index adaptors with UMIs, packaged in a single-use 96-well plate with a pierceable foil seal. Universal primers are included for applications requiring PCR amplification.
NEBNext Unique Dual Index UMI Adaptors for RNA-seq (NEB # E7416) are also available, as well as single, dual, and unique dual index primers, plus an optimized adaptor.
For additional information on available indices and formats for multiplexing, please refer to the NEBNext Multiplex Oligos Selection Chart.
NEBNext® Multiplex Oligos for Illumina® (Unique Dual Index UMI Adaptors DNA Set 1) are built for more streamlined and informative NGS multiplexing, tracking the sequencing reaction at the individual molecule level and enabling bioinformatic removal of misassigned reads for optimal accuracy. These index adaptors contain a pair of unique dual indices (i5 and i7) and one UMI sequence, making the resulting library multiplex-ready, with or without PCR. This set includes 96 unique dual index UMI adaptors, packaged in a single-use 96-well plate with a pierceable foil seal. The universal primer mix is supplied in a separate vial.
Combining UMI Adaptors DNA Set 1 with one of the NEBNext DNA library prep kits is simple and straightforward if you follow the protocols found in the library prep kit manuals. Quickly find the correct manual by selecting which library prep kit/module you plan to use:
The NEBNext line of Multiplex Oligos can be used with NEBNext sample prep products or other standard Illumina-compatible library preparation protocols. In addition to this set, additional index adaptors with UMIs are available for RNA-seq (NEB #E7416). For multiplexing without UMIs, NEBNext offers several options for added flexibility, including unique dual index primers (NEB #6440, NEB #6442, NEB #E6444, and NEB #E6446), dual index primers (NEB #E7600 and NEB #E7780), and single primer sets (in 12- and 96-index formats; NEB #E7335, NEB #E7500, NEB #E7710, NEB #E7730, NEB #E6609), as well as an option compatible with EM-seq™ and bisulfite sequencing (NEB #E7140).
The following reagents are supplied with this product:
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