New England Biolabs is working diligently to ensure we keep our employees and their families safe, while maintaining our business continuity. Despite a "Stay At Home" advisory being put in place in Massachusetts, USA, we are deemed an essential business, and our manufacturing and distribution teams continue to be fully operational. We are continuing to work with our suppliers and distribution partners to ensure uninterrupted access to our products and technical support.

NEB® PCR Cloning Kit

New! Updated to allow for in vitro transcription. Also available without competent cells.

Now supplied with outgrowth medium optimized for NEB 10-beta & NEB Stable Competent E.coli ; please do not use SOC outgrowth medium.

Easy cloning of all PCR products, including blunt and TA ends

  • Updated to allow for in vitro transcription with both SP6 and T7 promoters
  • BsaI-site removed from the ampicillin-resistant gene (allows for the cloning of Golden Gate Assembly modules)
  • Fast cloning experiments with 5-minute ligation step
  • Simplified screening with low/no colony background and no blue/white selection required
  • Save time by eliminating purification steps
  • Flanking restriction sites available for easy subcloning, including choice of two single digest options
  • Provided analysis primers allow for downstream colony PCR screening or sequencing
  • Ready-to-use kit components include 1 kb control amplicon, linearized cloning vector and single-use competent E. coli
  • Longer shelf life (12 months), as compared to some commercially available products
  • Includes competent cells
  • More restriction sites added, including four 8-base cutters
Learn more about how this product is being used in the Product Citation Tool
Catalog # Concentration Size List Price Your Price Quantity
E1202S Not Applicable 20 reactions $284.00
Please enter a quantity for at least one size

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