HomeFAQsCan Cloning Mix 1 and Cloning Mix 2 be mixed together before adding them to the ligation reaction?
FAQ: Can Cloning Mix 1 and Cloning Mix 2 be mixed together before adding them to the ligation reaction?
Cloning Mix 1 and 2 can be mixed before setting up the reaction. For long term storage Cloning Mix 1 and Cloning Mix 2 should be stored in their separate vials. Storing mixed Cloning Mix 1 and 2 together will result in reduced enzyme activity.
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