Home FAQs What is the difference between the original pMiniT and the pMiniT 2.0 linearized vector backbone now provided in the kit?

FAQ: What is the difference between the original pMiniT and the pMiniT 2.0 linearized vector backbone now provided in the kit?

The 2.0 version features three improvements:

  1. Addition of T7 and SP6 RNA polymerase promoter sequences flanking the cloning site for in vitro transcription of cloned inserts.
  2. Addition of seven new restriction sites including four 8 base recognition restriction enzyme sites flanking the cloning site to allow easier linearization downstream of the cloned insert for transcription studies, and to increase options for subcloning strategies
  3. Elimination of the BsaI site present in the ApR gene through site-directed mutagenesis to allow easier downstream use of cloned inserts/modules for Golden Gate Assembly.

Both versions of the linearized vector backbone have identical functionality in the cloning kit.