HomeFAQsDo these polishing components present in the master mix affect my cloning efficiency if my insert already has blunt ends?
FAQ: Do these polishing components present in the master mix affect my cloning efficiency if my insert already has blunt ends?
No. The concentrations of the end-polishing components are carefully optimized so that any single base overhangs will be removed to form blunt ends; already blunt-ended PCR products are not affected.
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