FAQ: How can the cloning vector work with both blunt-ended amplicons and single-base overhang-containing amplicons?

The mixes contain end-polishing components that will convert single-base overhangs, such as those present in amplicons made by Taq DNA polymerase or Taq-based mixes, to blunt ends. The blunt-end form then can be ligated into the pMiniT 2.0 vector.