For added convenience and accuracy in your ligation reaction setup, try our DNA Ligase Master Mixes
. These pre-mixed, ready-to-use formulations include a proprietary ligation reaction enhancement agent for improved performance. For your convenience, master mixes are available for every end type: Instant Sticky-end Ligase Master Mix (NEB #M0370
) for sticky ends, and Blunt/TA Ligase Master Mix (NEB #M0367
) for blunt or single-base overhang ends.
Learn more about NEB's quality controls for DNA ligases.
For help with selecting or using a DNA ligase, visit our supporting materials:
NEBcloner, an interactive tool for selecting appropriate products and viewing protocols for steps in the cloning workflow
DNA Ligase Selection Chart
Substrate-based Ligase Selection Chart
Properties of DNA and RNA Ligases Chart
Ligase Troubleshooting Guide
App note: Joining Difficult to Ligate dsDNA Fragments
App note: Efficient Adaptor Ligation for the Preparation of dsDNA Libraries using Blunt/TA Ligase Master Mix
Electroligase® is a registered trademark of New England Biolabs.
Quick Ligation™ is a trademark of New England Biolabs
SilverXpress™is a trademark of Life Technologies, Inc.