For added convenience and accuracy in your ligation reaction setup, try our new DNA Ligase Master Mixes. These pre-mixed, ready-to-use formulations include a proprietary ligation reaction enhancement agent for improved performance. Learn more about Joining Difficult to Ligase dsDNA Fragments and Efficient Adaptor Ligation for the Preparation of dsDNA Libraries using Blunt/TA Ligase Master Mix.
For your convenience, master mixes are available for every end type:
- For sticky ends, try our Instant Sticky-end Ligase Master Mix (NEB #M0370)
- For blunt or single-base overhang ends, try our Blunt/TA Ligase Master Mix (NEB #M0367)
For the full list of DNA Ligases and Ligase Master Mixes offered by NEB, as well as suggested applications, visit
the DNA Ligase Selection Chart.
For information about properties of DNA and RNA Ligases, visit the Properties of DNA and RNA Ligases Chart.
For additional help, visit our Ligase Troubleshooting Guide
For details on NEB's quality controls for DNA ligases, visit our Ligase Quality
is a guide for selecting appropriate products and viewing protocols for steps in the cloning workflow. To help select the right ligase, choose "Ligation" on the tool to start.
Electroligase® is a registered trademark of New England Biolabs.
Quick Ligation™ is a trademark of New England Biolabs
SilverXpress™is a trademark of Life Technologies, Inc.