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DNA Ligase Products

The largest selection of DNA Ligases for your ligation needs

With over 40 years of experience in the development and production of enzymes for molecular biology, NEB offers the most extensive selection of high-quality and performance-optimized DNA ligases and master mixes to streamline your experiments.

A few of our more popular standalone ligases and ligase kits available are:

For added convenience and accuracy in your ligation reaction setup, try our DNA Ligase Master Mixes. These pre-mixed, ready-to-use formulations include a proprietary ligation reaction enhancement agent for improved performance. For your convenience, master mixes are available for every end type: Instant Sticky-end Ligase Master Mix (NEB #M0370) for sticky ends, and Blunt/TA Ligase Master Mix (NEB #M0367) for blunt or single-base overhang ends. 

Learn more about NEB's quality controls for DNA ligases.

For help with selecting or using a DNA ligase, visit our supporting materials:
  • NEBcloner, an interactive tool for selecting appropriate products and viewing protocols for steps in the cloning workflow
  • DNA Ligase Selection Chart
  • Substrate-based Ligase Selection Chart
  • Properties of DNA and RNA Ligases Chart
  • Ligase Troubleshooting Guide
  • App note: Joining Difficult to Ligate dsDNA Fragments
  • App note: Efficient Adaptor Ligation for the Preparation of dsDNA Libraries using Blunt/TA Ligase Master Mix

  • Blunt/TA Ligase Master Mix improves yields for ends that typically react slowly

    Yields of final ligation product for all reaction conditions using high concentration T4 DNA Ligase (NEB #M0202), the Quick Ligation Kit (NEB #M2200) and Blunt/TA Master Mix (NEB #M0367). Nick, cohesive end and 3´ single-base overhang substrates were incubated for 15 minutes; the 5´ single-base overhang was incubated for 1 hour.

    Instant Sticky-end Ligase Master Mix Offers Robust Ligation with No Incubation Step

    Reactions containing 20 ng of pUC19 digested with SacI/SphI, and a 3-foldmolar excess of a 1.6 kb fragment from a different plasmid with compatible ends were set up using the Instant Sticky-end Ligase Master Mix. Without any additional incubation time, 2 µl were immediately withdrawn and used to transform a 50 µl aliquot of NEB 10-beta Competent E. coli (NEB #C3019). A 50 µl aliquot of the 1 ml outgrowth was plated onto a selective plate and incubated overnight at 37°C. Results show that several hundred colonies wereproduced.

    ElectroLigase reactions are complete in 60 minutes or less

    Ligation reactions containing equal amounts (20 ng vector and 3-fold molar excess of insert) of blunt (A) or T/A (B) vector/insert pairs were set up using ElectroLigase and incubated for the times shown. After heat inactivation of the ligase, 2 µl of each reaction were withdrawn and directly used to transform NEB 10-beta Electrocompetent E. coli (NEB #C3020). 50 µl aliquots of the outgrowth (diluted, in some cases) was plated onto selective plates and incubated overnight at 37°C. Colonies were counted, adjusted for plating dilution, and graphed.

    Electroligase® is a registered trademark of New England Biolabs.
    Quick Ligation™ is a trademark of New England Biolabs
    SilverXpress™is a trademark of Life Technologies, Inc.

    Protocols for DNA Ligase Products

    Reported Activities and Applications for T4 Ligases

    Relative Efficiency of T4 Ligases on Nicked Substrates

    Legal Information

    This product is covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB).

    While NEB develops and validates its products for various applications, the use of this product may require the buyer to obtain additional third party intellectual property rights for certain applications.

    For more information about commercial rights, please contact NEB's Global Business Development team at

    This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

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      High fidelity polymerases are everywhere—but why would you need a high fidelity ligase? And what do we even mean by “fidelity” when we’re talking about ligation? In this webinar, NEB Scientist and ligase expert Greg Lohman discusses mismatch ligation by DNA ligases and the molecular diagnostics applications that depend on the use of high-fidelity DNA ligases like NEB’s HiFi Taq DNA Ligase to detect single base differences in DNA.

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      Ligation, the process of joining DNA fragments with a DNA ligase, proceeds in three steps. Learn more about ligation with our quick animation.

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      Polyethylene glycol (PEG) is an important reagent in ligation reactions, find out why.

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      NEB continues to develop and produce the most extensive commercially available selection of high-quality, and performance-optimized DNA ligases and ligase master mixes for your ligation needs.

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      Find out how the downstream application dictates the best reaction conditions for ligation.

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      The optimal reactant ratio is contingent upon the downstream application.