The largest selection of DNA Ligases for your ligation needs
With over 40 years of experience in the development and production of enzymes for molecular biology, NEB offers the most extensive selection of high-quality and performance-optimized DNA ligases and master mixes to streamline your experiments.
A few of our more popular standalone ligases and ligase kits available are:
Electroligase® is a registered trademark of New England Biolabs.
Quick Ligation™ is a trademark of New England Biolabs
SilverXpress™is a trademark of Life Technologies, Inc.
Protocols for DNA Ligase Products
- E. coli DNA Ligase Protocol (M0205)
- Protocol for Taq DNA Ligase (M0208)
- Protocol for 9°N DNA Ligase (M0238)
- Transformation Protocol (M0369)
- Ligation Protocol for Cloning with ElectroLigase® (M0369)
- Ligation Protocol with T4 DNA Ligase (M0202)
- Ligation Protocol for Cloning with Instant Sticky-end Ligase Master Mix (M0370)
- Ligation Protocol for Cloning with Blunt/TA Ligase Master Mix (M0367)
- Transformation Protocol (M0367)
- Transformation Protocol (M0370)
- Ligation Protocol with T3 DNA Ligase (M0317)
- Ligation Protocol with T7 DNA Ligase (M0318)
- HiFi Taq DNA Ligase (M0647) Protocol
- Quick Ligation Protocol (M2200)
- Transformation Protocol
- Ligation protocol using SplintR® Ligase (M0375)
- Protocol for ssDNA/RNA Ligation (M0319)
- NEBNext Quick Ligation Module Protocol (E6056)
- Efficient Adaptor Ligation for the Preparation of dsDNA Libraries using the Blunt/TA Ligase Master Mix
- Joining of Difficult to Ligate dsDNA Fragments with Blunt TA Ligase Master Mix
DNA Ligase Brochure
The DNA Ligase brochure provides information about the extensive selection of DNA ligases and ligases master mixes available from NEB.
Substrate specificity and mismatch discrimination in DNA ligases
DNA ligases vary in their ability to join fragments, add adaptors, repair nicks or breaks, link vectors and inserts, and to circularize dsDNA. Ligases also vary in their type of activity. The specificity and accuracy of the ligation depends upon ligase selection and careful optimization of reaction conditions. With the right ligase, conditions and probes, even single-base variations in sequence can be reliably detected.
- DNA Ligase Selection Chart
- Properties of DNA and RNA Ligases
- Troubleshooting Guide for Cloning
- Troubleshooting Guide for Ligases
- Troubleshooting Tips for Ligation Reactions
- Tips for Maximizing Ligation Efficiencies
- Traditional Cloning Quick Guide
Other Tools & Resources
Reported Activities and Applications for T4 Ligases
Relative Efficiency of T4 Ligases on Nicked Substrates
While NEB develops and validates its products for various applications, the use of this product may require the buyer to obtain additional third party intellectual property rights for certain applications.
For more information about commercial rights, please contact NEB's Global Business Development team at firstname.lastname@example.org.
This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.
High fidelity polymerases are everywhere—but why would you need a high fidelity ligase? And what do we even mean by “fidelity” when we’re talking about ligation? In this webinar, NEB Scientist and ligase expert Greg Lohman discusses mismatch ligation by DNA ligases and the molecular diagnostics applications that depend on the use of high-fidelity DNA ligases like NEB’s HiFi Taq DNA Ligase to detect single base differences in DNA.
Ligation, the process of joining DNA fragments with a DNA ligase, proceeds in three steps. Learn more about ligation with our quick animation.
Ligation of blunt ends and single-base overhangs require optimized reaction conditions.
Polyethylene glycol (PEG) is an important reagent in ligation reactions, find out why.
NEB continues to develop and produce the most extensive commercially available selection of high-quality, and performance-optimized DNA ligases and ligase master mixes for your ligation needs.
Find out how the downstream application dictates the best reaction conditions for ligation.
The optimal reactant ratio is contingent upon the downstream application.