Ligase banner

DNA Ligase Products

The largest selection of DNA Ligases for your ligation needs

With over 45 years of experience in the development and production of enzymes for molecular biology, NEB offers the most extensive selection of high-quality and performance-optimized DNA ligases and master mixes to streamline your experiments.

A few of our more popular standalone ligases and ligase kits available are:

For added convenience and accuracy in your ligation reaction setup, try our DNA Ligase Master Mixes. These pre-mixed, ready-to-use formulations include a proprietary ligation reaction enhancement agent for improved performance. For your convenience, master mixes are available for every end type: Instant Sticky-end Ligase Master Mix (NEB #M0370) for sticky ends, and Blunt/TA Ligase Master Mix (NEB #M0367) for blunt or single-base overhang ends. 

Learn more about NEB's quality controls for DNA ligases.

For help with selecting or using a DNA ligase, visit our supporting materials:
  • NEBcloner, an interactive tool for selecting appropriate products and viewing protocols for steps in the cloning workflow
  • DNA Ligase Selection Chart
  • Substrate-based Ligase Selection Chart
  • Properties of DNA and RNA Ligases Chart
  • Ligase Troubleshooting Guide
  • App note: Joining Difficult to Ligate dsDNA Fragments
  • App note: Efficient Adaptor Ligation for the Preparation of dsDNA Libraries using Blunt/TA Ligase Master Mix

  • Ligase Fidelity

    Extensive research has been conducted at NEB regarding the end-joining ligase fidelity (discrimination against ligating mismatched overhangs) and bias (sequence preference) for short, cohesive ends.  This information can be used to optimize design of Golden Gate Assembly and achieve high efficiency and high fidelity assemblies of 35+ fragments. 

    NEB has compiled this information into the following tools:

    Ligase Fidelity Viewer (v2)
    Visualize overhang ligation preferences

    Predict high-fidelity junction sets

    Split DNA sequence for scarless high-fidelity assembly

    For more information on ligase fidelity and Golden Gate assembly, watch this webinar or visit


    SilverXpress is a trademark of Life Technologies, Inc.

    Choose Type:

    Protocols for DNA Ligase Products
    Application Notes for DNA Ligase Products
    Reported Activities and Applications for T4 Ligases
    Relative Efficiency of T4 Ligases on Nicked Substrates
    Legal Information

    This product is covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB).

    While NEB develops and validates its products for various applications, the use of this product may require the buyer to obtain additional third party intellectual property rights for certain applications.

    For more information about commercial rights, please contact NEB's Global Business Development team at

    This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.