The largest selection of DNA Ligases for your ligation needs
With over 40 years of experience in the development and production of enzymes for molecular biology, NEB offers the most extensive selection of high-quality and performance-optimized DNA ligases and master mixes to streamline your experiments.
A few of our more popular standalone ligases and ligase kits available are:
Electroligase® is a registered trademark of New England Biolabs.
Quick Ligation™ is a trademark of New England Biolabs
SilverXpress™is a trademark of Life Technologies, Inc.
Protocols for DNA Ligase Products
- Ligation Protocol with T3 DNA Ligase (M0317)
- Ligation Protocol with T7 DNA Ligase (M0318)
- E. coli DNA Ligase Protocol (M0205)
- Protocol for Taq DNA Ligase (M0208)
- Protocol for 9°N DNA Ligase (M0238)
- Ligation Protocol for Cloning with Instant Sticky-end Ligase Master Mix (M0370)
- Ligation Protocol for Cloning with Blunt/TA Ligase Master Mix (M0367)
- Transformation Protocol (M0367)
- Transformation Protocol (M0370)
- Transformation Protocol (M0369)
- Ligation Protocol for Cloning with ElectroLigase® (M0369)
- Ligation Protocol with T4 DNA Ligase (M0202)
- HiFi Taq DNA Ligase (M0647) Protocol
- Protocol for ssDNA/RNA Ligation (M0319)
- Transformation Protocol
- Quick Ligation Protocol (M2200)
- Ligation protocol using SplintR® Ligase (M0375)
- NEBNext Quick Ligation Module Protocol (E6056)
Application Notes for DNA Ligase Products
DNA Ligase Brochure
The DNA Ligase brochure provides information about the extensive selection of DNA ligases and ligases master mixes available from NEB.
- DNA Ligase Selection Chart
- Properties of DNA and RNA Ligases
- Troubleshooting Guide for Cloning
- Troubleshooting Guide for Ligases
- Troubleshooting Tips for Ligation Reactions
- Tips for Maximizing Ligation Efficiencies
- Traditional Cloning Quick Guide
Other Tools & Resources
Reported Activities and Applications for T4 Ligases
Relative Efficiency of T4 Ligases on Nicked Substrates
High fidelity polymerases are everywhere—but why would you need a high fidelity ligase? And what do we even mean by “fidelity” when we’re talking about ligation? In this webinar, NEB Scientist and ligase expert Greg Lohman discusses mismatch ligation by DNA ligases and the molecular diagnostics applications that depend on the use of high-fidelity DNA ligases like NEB’s HiFi Taq DNA Ligase to detect single base differences in DNA.
Ligation, the process of joining DNA fragments with a DNA ligase, proceeds in three steps. Learn more about ligation with our quick animation.
Ligation of blunt ends and single-base overhangs require optimized reaction conditions.
Polyethylene glycol (PEG) is an important reagent in ligation reactions, find out why.
NEB continues to develop and produce the most extensive commercially available selection of high-quality, and performance-optimized DNA ligases and ligase master mixes for your ligation needs.
Find out how the downstream application dictates the best reaction conditions for ligation.
The optimal reactant ratio is contingent upon the downstream application.