The largest selection of DNA Ligases for your ligation needs
With over 45 years of experience in the development and production of enzymes for molecular biology, NEB offers the most extensive selection of high-quality and performance-optimized DNA ligases and master mixes to streamline your experiments.
A few of our more popular standalone ligases and ligase kits available are:
Extensive research has been conducted at NEB regarding the end-joining ligase fidelity (discrimination against ligating mismatched overhangs) and bias (sequence preference) for short, cohesive ends. This information can be used to optimize design of Golden Gate Assembly and achieve high efficiency and high fidelity assemblies of 35+ fragments.
NEB has compiled this information into the following tools:
Ligase Fidelity Viewer™ (v2)
Visualize overhang ligation preferences
Predict high-fidelity junction sets
Split DNA sequence for scarless high-fidelity assembly
SilverXpress™ is a trademark of Life Technologies, Inc.
- Ligation Protocol with T4 DNA Ligase (M0202)
- NEBNext Quick Ligation Module Protocol (E6056)
- Quick Ligation Protocol (M2200)
- Transformation Protocol
- Transformation Protocol (M0367)
- Transformation Protocol (M0370)
- Ligation Protocol for Cloning with Instant Sticky-end Ligase Master Mix (M0370)
- Ligation Protocol for Cloning with Blunt/TA Ligase Master Mix (M0367)
- Ligation Protocol for Cloning with ElectroLigase® (M0369)
- Transformation Protocol (M0369)
- Protocol for ssDNA/RNA Ligation (M0319)
- Ligation Protocol with T3 DNA Ligase (M0317)
- Ligation Protocol with T7 DNA Ligase (M0318)
- Ligation protocol using SplintR® Ligase (M0375)
- E. coli DNA Ligase Protocol (M0205)
- Protocol for 9°N DNA Ligase (M0238)
- Protocol for Taq DNA Ligase (M0208)
- HiFi Taq DNA Ligase (M0647) Protocol
- Protocol for Salt-T4® DNA Ligase (NEB #M0467)
- Protocol for Hi-T4™ DNA Ligase (NEB #M2622)
- DNA Ligase Selection Chart
- Properties of DNA and RNA Ligases
- Troubleshooting Guide for Cloning
- Troubleshooting Guide for Ligases
- Troubleshooting Tips for Ligation Reactions
- Tips for Maximizing Ligation Efficiencies
- Traditional Cloning Quick Guide
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This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.
Ligase Fidelity and Bias in End-Joining Ligation: Enabling complex, multi-fragment Golden Gate DNA Assembly
In this webinar, you will learn about our recently developed a single-molecule sequencing method to characterize end-joining ligase fidelity (discrimination against ligating mismatched overhangs) and bias (sequence preferences) for short cohesive ends in a high throughput manner. This method allows determination of the relative frequency of all ligation products with or without mismatches, the position-dependent frequency of each mismatch, and sequence-dependent biases in ligation efficiency. We have applied the fidelity and bias data to optimize design of Golden Gate-type assemblies, allowing selection of overhang sets with minimized mismatch potential and high efficiency.
High fidelity polymerases are everywhere—but why would you need a high fidelity ligase? And what do we even mean by “fidelity” when we’re talking about ligation? In this webinar, NEB Scientist and ligase expert Greg Lohman discusses mismatch ligation by DNA ligases and the molecular diagnostics applications that depend on the use of high-fidelity DNA ligases like NEB’s HiFi Taq DNA Ligase to detect single base differences in DNA.
Ligation, the process of joining DNA fragments with a DNA ligase, proceeds in three steps. Learn more about the function of ligation with our quick tutorial animation.
Ligation of blunt ends and single-base overhangs require optimized reaction conditions.
Polyethylene glycol (PEG) is an important reagent in ligation reactions, find out why.
Find out how the downstream application dictates the best reaction conditions for ligation.
The optimal reactant ratio is contingent upon the downstream application.