Substrate-based Ligase Selection Chart
This chart provides our recommendation for a choice of ligase to use in a reaction, based upon the type of substrate present. DNA, RNA and hybrid substrates are represented and require specific enzymes to achieve the highest-efficiency ligation. The DNA Ligase Selection Chart helps you select the optimal DNA ligase for various applications. In addition, the Properties of DNA and RNA Ligases will help you understand the differences between them.
RECOMMENDED LIGASE | COMMENTS | |
---|---|---|
NICKED DNA/RNA | ||
T4 RNA Ligase 2 | ||
T4 RNA Ligase 2 | ||
T4 RNA Ligase 2 | ||
T4 DNA Ligase Immobilized T4 DNA Ligase |
||
N/A | No ligase optimized for this activity | |
T3 DNA Ligase | ||
SplintR Ligase | 100-1,000-fold higher efficiency than T4 DNA Ligase | |
T4 DNA Ligase Immobilized T4 DNA Ligase |
For high temperatures, we recommend Taq DNA Ligase. For highest fidelity, we recommend HiFi Taq DNA Ligase. | |
ssDNA/RNA | ||
N/A | See CircLigase™ | |
N/A | No ligase optimized for this activity | |
T4 RNA Ligase 1 | Supplement with ATP | |
T4 RNA Ligase 1 | Supplement with ATP | |
T4 RNA Ligase 2 Truncated KQ | ||
T4 RNA Ligase 2 Truncated KQ | ||
Thermostable 5´ App DNA/RNA Ligase | We recommend a Proteinase K cleanup | |
Thermostable 5´ App DNA/RNA Ligase | We recommend a Proteinase K cleanup | |
RtcB Ligase | Supplement with GTP and Mn2+ | |
RtcB Ligase | Supplement with GTP and Mn2+ | |
T4 RNA Ligase 1 | ||
T4 RNA Ligase 1 | Reported to work, but ligates inefficiently. Consider pdCp. | |
T4 RNA Ligase 1 | ||
T4 RNA Ligase 1 | ||
dsDNA | ||
Blunt T/A Ligase Master Mix | ||
Blunt T/A Ligase Master Mix | ||
Quick Ligation Kit or Instant Sticky-end Ligase Master Mix | For ligating ends under high salt conditions, we recommend Salt-T4 DNA Ligase. For ligation at temperatures up to 50°C, we recommend Hi-T4 DNA Ligase. For ligation of cohesive ends ONLY, we recommend T7 DNA Ligase. | |
Quick Ligation Kit or Instant Sticky-end Ligase Master Mix | For ligating ends under high salt conditions, we recommend Salt-T4 DNA Ligase. For ligation at temperatures up to 50°C, we recommend Hi-T4 DNA Ligase. For ligation of cohesive ends ONLY, we recommend T7 DNA Ligase. |
CIRCLIGASE™ is a trademark of EpiCentre Technologies Corp.